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Mouse Monoclonal beta Actin antibody. Suitable for WB, ICC/IF and reacts with Chinese hamster, Mouse, Rat, African green monkey, Human, Dog, Cow samples. Cited in 2378 publications. Immunogen corresponding to Synthetic Peptide within Human Beta-actin aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Alternative names=Beta-actin, ACTB
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Synthetic Peptide within Human Beta-actin aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link: P60709
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| African green monkey | Tested | Expected |
| Chinese hamster | Tested | Expected |
| Cow | Tested | Expected |
| Dictyostelium discoideum | Not recommended | Not recommended |
| Dog | Tested | Expected |
| Drosophila melanogaster | Not recommended | Not recommended |
Images
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Mouse | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Rat | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species African green monkey | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Human | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Dog | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Cow | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Dictyostelium discoideum, Drosophila melanogaster | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Mouse, Rat, Dog, Cow, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Dictyostelium discoideum, Drosophila melanogaster | Dilution info - | Notes - |
Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).
Recommended products
Mouse Monoclonal beta Actin antibody. Suitable for WB, ICC/IF and reacts with Chinese hamster, Mouse, Rat, African green monkey, Human, Dog, Cow samples. Cited in 2378 publications. Immunogen corresponding to Synthetic Peptide within Human Beta-actin aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Alternative names=Beta-actin, ACTB
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Synthetic Peptide within Human Beta-actin aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link: P60709
- Clone number
AC-15
- Purification technique
Affinity purification
- Epitope
N-terminal of the beta isoform of actin.
- Concentration
- Purification notes
Purified from hybridoma cell culture.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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