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Anti-beta Actin antibody [AC-15] is a mouse monoclonal antibody that is used to detect beta Actin in ICC/IF, Western blot. Suitable for African green monkey, Chinese hamster, Cow, Dog, Human, Mouse, Rat samples.

- Specificity confirmed with ACTB knockout cell line validation
- Cited in over 2,370 publications


Images

Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276), expandable thumbnail
  • Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] (AB6276), expandable thumbnail
  • Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276), expandable thumbnail
  • Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P60709

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
African green monkey
Tested
Expected
Chinese hamster
Tested
Expected
Cow
Tested
Expected
Dictyostelium discoideum
Not recommended
Not recommended
Dog
Tested
Expected
Drosophila melanogaster
Not recommended
Not recommended

Tested
Tested

Species
Chinese hamster
Dilution info
1/5000.00000 - 1/16000.00000
Notes

-

Species
Mouse
Dilution info
1/5000.00000 - 1/16000.00000
Notes

-

Species
Rat
Dilution info
1/5000.00000 - 1/16000.00000
Notes

-

Species
African green monkey
Dilution info
1/5000.00000 - 1/16000.00000
Notes

-

Species
Human
Dilution info
1/5000.00000 - 1/16000.00000
Notes

-

Species
Dog
Dilution info
1/5000.00000 - 1/16000.00000
Notes

-

Species
Cow
Dilution info
1/5000.00000 - 1/16000.00000
Notes

-

Not recommended
Not recommended

Species
Dictyostelium discoideum, Drosophila melanogaster
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Chinese hamster, Mouse, Rat, Dog, Cow, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Dictyostelium discoideum, Drosophila melanogaster
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

8 products for Alternative Product

Target data

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

Alternative names

Recommended products

Anti-beta Actin antibody [AC-15] is a mouse monoclonal antibody that is used to detect beta Actin in ICC/IF, Western blot. Suitable for African green monkey, Chinese hamster, Cow, Dog, Human, Mouse, Rat samples.

- Specificity confirmed with ACTB knockout cell line validation
- Cited in over 2,370 publications

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
Clone number
AC-15
Purification technique
Affinity purification
Epitope
N-terminal of the beta isoform of actin.
Concentration
Loading...
Purification notes

Purified from hybridoma cell culture.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-beta Actin antibody [AC-15] (ab6276) is a mouse monoclonal antibody and is validated for use in ICC/IF and WB.

Anti-beta Actin antibody [AC-15] (ab6276) was first used in a scientific publication in 1994 and has been cited over 2378 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-beta Actin antibody [AC-15] (ab6276) has high sensitivity and specificity.

The specificity of Anti-beta Actin antibody [AC-15] (ab6276) has been confirmed by WB testing in beta Actin knockout HAP1 cells.

Anti-beta Actin antibody [AC-15] (ab6276) has 94 independent reviews from customers.

beta Actin antibodies are often used as loading controls in Western Blot. Anti-beta Actin antibody [AC-15] has been verified in Western Blot samples and detects a band at 42kDa Molecular weight.

Anti-beta Actin antibody [AC-15] (ab6276) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 50 µL selling sizes.

Antibody clone AC-15 is also available pre-conjugated to a variety of labels for your convenience - HRP, FITC (ab499, FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker ab6277).

Beta-Actin (β-actin) is a highly conserved protein that plays a crucial role in cell motility, structure and integrity. It is one of the six actin isoforms found in humans and is a major component of the cytoskeleton in non-muscle cells. β-actin is involved in various cellular processes, including cell division, signaling and maintaining cell shape. Due to its ubiquitous expression and stability, β-actin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding β-actin's functions and interactions is essential for studying cellular dynamics and various physiological processes.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Associated diseases and disorders

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail

    Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)

    Beta Actin Western blot staining using mouse Anti-beta Actin antibody

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Beta actin knockout HAP1 cell lysate (20 μg)

    Lanes 1 and 2: Merged signal (red and green). Green - beta actin, ab6276 observed at 42 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa.

    ab6276 was shown to specifically react with beta actin in wild-type HAP1 cells. No band was observed when beta actin knockout samples were used. Wild-type and beta actin knockout samples were subjected to SDS-PAGE. ab6276 (beta actin) and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted 1/5000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    Lanes 1 - 2: Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276) at 1/5000 dilution

    Lanes 1 - 2: Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) at 1/10000 dilution

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Beta actin knockout HAP1 cell lysate (20 µg)

    Secondary

    Lanes 1 - 2: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/10000 dilution

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

    Predicted band size: 36 kDa, 41 kDa

  • Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Mark Elliott

    Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)

    Beta Actin Western blot staining using mouse Anti-beta Actin antibody

    Western Blot of ab6276 (used as loading control) with whole tissue lysate of human grey matter from BA20 (temporal cortex). ab6276 was diluted 1/50000 and incubated with the sample for 16 hours at 4°C. 5 μg of lysate was loaded onto the gel, which was blocked with 5% milk for 1 hour at 20°C. An Alexa Fluor® 680 conjugated goat anti-mouse antibody, diluted 1/5000, was used as the secondary.

    Bands below actin in image are synaptophysin (SYN).

    All lanes: Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)

    Predicted band size: 41 kDa

    Observed band size: 42 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] (ab6276), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] (ab6276)

    ab6276 staining beta Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab6276 at a working concentration of 5μg/ml and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 647, shown in red) at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor® 488 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail

    Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)

    Beta Actin Western blot staining using mouse Anti-beta Actin antibody

    All lanes: Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276) at 1 µg/mL

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Lane 3: COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate

    Lane 4: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

    Lane 5: PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

    Lane 6: Rat2 (rat fibroblast cell line) cell lysate

    Lane 7: CHO (chinese hamster ovary cell line) cell lysate

    Lane 8: MDBK (bovine kidney cell line) cell lysate

    Lane 9: MDCK (canine kidney cell line) cell lysate

    Secondary

    All lanes: Goat Anti-Mouse IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 41 kDa

  • Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail

    Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)

    Beta Actin Western blot staining using mouse Anti-beta Actin antibody

    All lanes: Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276) at 1/5000 dilution

    Lane 1: HeLa nuclear at 20 µg

    Lane 2: HeLa whole cell lysate at 20 µg

    Lane 3: A431 cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Lane 5: HEK293 cell lysate at 20 µg

    Secondary

    All lanes: Alexa Fluor anti mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 41 kDa

    Observed band size: 42 kDa

  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276), expandable thumbnail
    This image is courtesy of David Grimm, Yale University, USA.

    Western blot - Anti-beta Actin antibody [AC-15] (ab6276)

    MDCK cells induced with increasing amounts of doxycycline to control expression of the gene of interest. All cells were normalized for loading with an albumin protein standard assay. Anti-beta actin (ab6276) was used at a concentration of 1:5000 in a milk blocking solution. B-actin blotting confirms the albumin assay in showing that an equal amount of lysate was loaded in each lane.

    All lanes: Western blot - Anti-beta Actin antibody [AC-15] (ab6276)

    Predicted band size: 41 kDa

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Product protocols

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