Anti-beta Actin antibody [AC-15] (ab6276) is a mouse monoclonal antibody detecting beta Actin in Western Blot, ICC/IF. Suitable for African green monkey, Chinese hamster, Cow, Dog, Human, Mouse, Rat.
- KO validated for confirmed specificity
- Over 2370 publications
- Trusted since 2002
Images
![Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--western-blot-img152293.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276)")
![Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--western-blot-img27516.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276)")
![Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--immunocytochemistry-immunofluorescence-img69893.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276)")
![Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--western-blot-img26221.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276)")
![Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--western-blot-img100694.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (AB6276)")
Publications
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- Scientific reports 13:226852023Evaluation of glomerular sirtuin-1 and claudin-1 in the pathophysiology of nondiabetic focal segmental glomerulosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuilherme Lopes-Gonçalves,Juliana Martins Costa-Pessoa,Ruan Pimenta,Ana Flavia Tostes,Eloisa Martins da Silva,Felipe Lourenço Ledesma,Denise Maria Avancini Costa Malheiros,Roberto Zatz,Karina Thieme,Niels Olsen Saraiva Câmara,Maria Oliveira-SouzaPubMed 38114708
- Scientific reports 13:211432023Oxidative stress and docosahexaenoic acid injury lead to increased necroptosis and ferroptosis in retinal pigment epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlmar Neiteler,Anwar A Palakkan,Kevin M Gallagher,James A RossPubMed 38036571
- Cell transplantation 32:96368972312133092023Neuroprotective Effects of Human Adipose-Derived Mesenchymal Stem Cells in Oxygen-Induced Retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJifu Xin,Lvlv Zhou,Ling Zhang,Kai Guo,Dayong YangPubMed 38018498
- Frontiers in molecular biosciences 10:12925552023Harnessing IGF-1 and IL-2 as biomarkers for calcineurin activity to tailor optimal FK506 dosage in α-synucleinopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSofia Zaichick,Gabriela CaraveoPubMed 38094080
- Aging 15:14607-146162023The protective effects of Phoenixin-20 in tumor necrosis factor α (TNF-α)-induced cell senescence of rheumatoid arthritis fibroblast-like synoviocytes (FLS).Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Yan,Ling Yao,Ying Tan,Yue WangPubMed 38112587
- BMC genomics 24:7152023Comprehensive exploration of the involvement of cuproptosis in tumorigenesis and progression of neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Zhou,Dongmei Huang,Wen Fu,Fangpeng ShuPubMed 38012558
- Cells 12:2023C5aR2 Regulates STING-Mediated Interferon Beta Production in Human Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliver Wright,Anna Harris,Van Dien Nguyen,You Zhou,Maxim Durand,Abbie Jayyaratnam,Darren Gormley,Luke A J O'Neill,Kathy Triantafilou,Eva Maria Nichols,Lee M BootyPubMed 38067135
- Journal of experimental & clinical cancer research : CR 42:3132023circ-hnRNPU inhibits NONO-mediated c-Myc transactivation and mRNA stabilization essential for glycosylation and cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongjun Li,Wanju Jiao,Jiyu Song,Jianqun Wang,Guo Chen,Dan Li,Xiaojing Wang,Banghe Bao,Xinyi Du,Yang Cheng,Chunhui Yang,Qiangsong Tong,Liduan ZhengPubMed 37993881
- Journal of neuroinflammation 20:2682023Cyanidin-3-O-glucoside protects the brain and improves cognitive function in APPswe/PS1ΔE9 transgenic mice model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHana Baek, Sanjay,Miey Park,Hae-Jeung LeePubMed 37978414
- Communications biology 6:11272023Synaptic proteasome is inhibited in Alzheimer's disease models and associates with memory impairment in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFelipe C Ribeiro,Danielle Cozachenco,Luana Heimfarth,Juliana T S Fortuna,Guilherme B de Freitas,Jorge M de Sousa,Soniza V Alves-Leon,Renata E P Leite,Claudia K Suemoto,Lea T Grinberg,Fernanda G De Felice,Mychael V Lourenco,Sergio T FerreiraPubMed 37935829
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| African green monkey | Tested | Expected |
| Chinese hamster | Tested | Expected |
| Cow | Tested | Expected |
| Dictyostelium discoideum | Not recommended | Not recommended |
| Dog | Tested | Expected |
| Drosophila melanogaster | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Mouse | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Rat | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species African green monkey | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Human | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Dog | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Species Cow | Dilution info 1/5000.00000 - 1/16000.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Dictyostelium discoideum, Drosophila melanogaster | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Mouse, Rat, Dog, Cow, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Dictyostelium discoideum, Drosophila melanogaster | Dilution info - | Notes - |
Associated Products
Select an associated product type
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8 products for Alternative Product
Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
Beta-actin, ACTB
Recommended products
Anti-beta Actin antibody [AC-15] (ab6276) is a mouse monoclonal antibody detecting beta Actin in Western Blot, ICC/IF. Suitable for African green monkey, Chinese hamster, Cow, Dog, Human, Mouse, Rat.
- KO validated for confirmed specificity
- Over 2370 publications
- Trusted since 2002
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
- Clone number
- AC-15
- Purification technique
- Affinity purification
- Epitope
- N-terminal of the beta isoform of actin.
- Concentration
- Purification notes
Purified from hybridoma cell culture.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-beta Actin antibody [AC-15] (ab6276) is a mouse monoclonal antibody and is validated for use in ICC/IF and WB.
Anti-beta Actin antibody [AC-15] (ab6276) was first used in a scientific publication in 1994 and has been cited over 2378 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-beta Actin antibody [AC-15] (ab6276) has high sensitivity and specificity.
The specificity of Anti-beta Actin antibody [AC-15] (ab6276) has been confirmed by WB testing in beta Actin knockout HAP1 cells.
Anti-beta Actin antibody [AC-15] (ab6276) has 94 independent reviews from customers.
beta Actin antibodies are often used as loading controls in Western Blot. Anti-beta Actin antibody [AC-15] has been verified in Western Blot samples and detects a band at 42kDa Molecular weight.
Anti-beta Actin antibody [AC-15] (ab6276) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 50 µL selling sizes.
Antibody clone AC-15 is also available pre-conjugated to a variety of labels for your convenience - HRP, FITC (ab499, FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker ab6277).
Beta-Actin (β-actin) is a highly conserved protein that plays a crucial role in cell motility, structure and integrity. It is one of the six actin isoforms found in humans and is a major component of the cytoskeleton in non-muscle cells. β-actin is involved in various cellular processes, including cell division, signaling and maintaining cell shape. Due to its ubiquitous expression and stability, β-actin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding β-actin's functions and interactions is essential for studying cellular dynamics and various physiological processes.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
- Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
- Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
- Associated diseases and disorders
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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6 product images
![Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--western-blot-img152293.jpg "Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)")
Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)
Beta Actin Western blot staining using mouse Anti-beta Actin antibody
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Beta actin knockout HAP1 cell lysate (20 μg)Lanes 1 and 2: Merged signal (red and green). Green - beta actin, ab6276 observed at 42 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa.
ab6276 was shown to specifically react with beta actin in wild-type HAP1 cells. No band was observed when beta actin knockout samples were used. Wild-type and beta actin knockout samples were subjected to SDS-PAGE. ab6276 (beta actin) and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted 1/5000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Lanes 1 - 2: Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276) at 1/5000 dilution
Lanes 1 - 2: Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) at 1/10000 dilution
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Beta actin knockout HAP1 cell lysate (20 µg)
SecondaryLanes 1 - 2: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/10000 dilution
Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution
Predicted band size: 36 kDa, 41 kDa
![Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--western-blot-img27516.jpg "Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)")
This image is courtesy of a customer review submitted by Dr Mark ElliottWestern blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)
Beta Actin Western blot staining using mouse Anti-beta Actin antibody
Western Blot of ab6276 (used as loading control) with whole tissue lysate of human grey matter from BA20 (temporal cortex). ab6276 was diluted 1/50000 and incubated with the sample for 16 hours at 4°C. 5 μg of lysate was loaded onto the gel, which was blocked with 5% milk for 1 hour at 20°C. An Alexa Fluor® 680 conjugated goat anti-mouse antibody, diluted 1/5000, was used as the secondary.
Bands below actin in image are synaptophysin (SYN).
All lanes: Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)
Predicted band size: 41 kDa
Observed band size: 42 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--immunocytochemistry-immunofluorescence-img69893.jpg "Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)")
Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)
Beta Actin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-beta Actin antibody
ab6276 staining beta Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab6276 at a working concentration of 5μg/ml and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 647, shown in red) at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor® 488 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).![Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--western-blot-img26221.jpg "Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)")
Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)
Beta Actin Western blot staining using mouse Anti-beta Actin antibody
All lanes: Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276) at 1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3: COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate
Lane 4: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 5: PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 6: Rat2 (rat fibroblast cell line) cell lysate
Lane 7: CHO (chinese hamster ovary cell line) cell lysate
Lane 8: MDBK (bovine kidney cell line) cell lysate
Lane 9: MDCK (canine kidney cell line) cell lysate
SecondaryAll lanes: Goat Anti-Mouse IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 41 kDa
![Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--western-blot-img100694.jpg "Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)")
Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)
Beta Actin Western blot staining using mouse Anti-beta Actin antibody
All lanes: Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276) at 1/5000 dilution
Lane 1: HeLa nuclear at 20 µg
Lane 2: HeLa whole cell lysate at 20 µg
Lane 3: A431 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Lane 5: HEK293 cell lysate at 20 µg
SecondaryAll lanes: Alexa Fluor anti mouse at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 42 kDa
![Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276), expandable thumbnail](https://content.abcam.com/products/images/beta-actin-antibody-ac-15-loading-control-ab6276--western-blot-img11309.gif "Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)")
This image is courtesy of David Grimm, Yale University, USA.Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)
Beta Actin Western blot staining using mouse Anti-beta Actin antibody
MDCK cells induced with increasing amounts of doxycycline to control expression of the gene of interest. All cells were normalized for loading with an albumin protein standard assay. Anti-beta actin (ab6276) was used at a concentration of 1:5000 in a milk blocking solution. B-actin blotting confirms the albumin assay in showing that an equal amount of lysate was loaded in each lane.
All lanes: Western blot - Anti-beta Actin antibody [AC-15] - Loading Control (ab6276)
Predicted band size: 41 kDa
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