Anti-beta Actin antibody [BLR057F] (ab252556)

Rabbit Recombinant Monoclonal beta Actin antibody. Suitable for IHC-P, ICC, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ACTB aa 1-50.

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Images

Immunocytochemistry - Anti-beta Actin antibody [BLR057F] (AB252556), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human ACTB aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P60709

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC	WB
Human	Tested	Tested	Tested
Mouse	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100.00000 - 1/500.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100.00000 - 1/500.00000	Notes Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Associated Products

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Target data

See full target information ACTB

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

Alternative names

Beta-actin, ACTB

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human ACTB aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
Clone number: BLR057F
Concentration
Purification notes: Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Associated diseases and disorders

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

Product promise

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15 product images

Immunocytochemistry - Anti-beta Actin antibody [BLR057F] (ab252556)
FFPE HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin using ab252556 at 1/200 dilution in ICC/IF, followed by HRP conjugated Goat Anti-Rabbit IgG. Substrate: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [BLR057F] (ab252556)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for beta Actin using ab252556 at 1/400 dilution in immunohistochemical analysis, followed by HRP conjugated. DAB staining.
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-beta Actin antibody [BLR057F] (ab252556) at 1/1000 dilution
Lane 1: U2-OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg
Lane 2: A549 (human lung carcinoma cell line) whole cell lysate at 50 µg
Lane 3: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 50 µg
Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 5: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 7: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 8: NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg
Secondary
All lanes: HRP conjugated Goat anti-Rabbit IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 1s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-CTCF antibody [BLR041F] (Anti-CTCF antibody [BLR041F] ab243897) at 1/1000 dilution
Lane 1: NIH 3T3 whole cell lysate at 50 µg
Lane 2: CT26 whole cell lysate at 50 µg
Lane 3: TCMK-1 whole cell lysate at 50 µg
Lane 4: BW5147.3 whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 130 kDa
Exposure time: 30s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-CD276 antibody [BLR025F] - BSA free (Anti-CD276 antibody [BLR025F] - BSA free ab243882) at 1/1000 dilution
Lane 1: NIH 3T3 whole cell lysate at 50 µg
Lane 2: AlphaTC1 Clone 9 whole cell lysate at 50 µg
Lane 3: NIH 3T3-L1 whole cell lysate at 50 µg
Lane 4: CT26 whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 46 kDa
Exposure time: 3min
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
Beta Actin Western blot staining using rabbit Anti-beta Actin antibody
All lanes: Western blot - Anti-CD44 antibody [BLR038F] (Anti-CD44 antibody [BLR038F] ab243894) at 1/1000 dilution
Lane 1: NIH 3T3 whole cell lysate at 10 µg
Lane 2: CT26 whole cell lysate at 10 µg
Lane 3: CH27 whole cell lysate at 10 µg
Lane 4: TCMK-1 whole cell lysate at 10 µg
Lane 5: BW5147.3 whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 85 kDa
Observed band size: 90 kDa
Exposure time: 30s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (Anti-hnRNP M3-M4 antibody [2A6-2H3] ab128143) at 1/1000 dilution
Lane 1: BW5147.3 whole cell lysate at 10 µg
Lane 2: CT26 whole cell lysate at 10 µg
Lane 3: CH27 whole cell lysate at 10 µg
Lane 4: TCMK-1 whole cell lysate at 10 µg
Lane 5: NIH 3T3 whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (Goat Anti-Mouse IgG H&L (HRP) ab97023)
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 80 kDa
Exposure time: 10s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-MDMX/MDM4 antibody [BL-3-2F2] (Anti-MDMX/MDM4 antibody [BL-3-2F2] ab243859) at 1/1000 dilution
Lane 1: Jurkat whole cell lysate at 50 µg
Lane 2: MCF-7 whole cell lysate at 50 µg
Lane 3: RKO whole cell lysate at 50 µg
Lane 4: U2OS whole cell lysate at 50 µg
Lane 5: HEK293T whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 70 kDa
Exposure time: 75s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-CD276 antibody [BLR025F] - BSA free (Anti-CD276 antibody [BLR025F] - BSA free ab243882) at 1/1000 dilution
Lane 1: MCF-7 whole cell lysate at 25 µg
Lane 2: Jurkat whole cell lysate at 25 µg
Lane 3: A-172 whole cell lysate at 25 µg
Lane 4: Ramos whole cell lysate at 25 µg
Lane 5: HeLa whole cell lysate at 25 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 110 kDa
Exposure time: 30s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (Anti-TRAP220/MED1 antibody [BLR037F] ab243893) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 10 µg
Lane 2: HEK293T whole cell lysate at 10 µg
Lane 3: MCF-7 whole cell lysate at 10 µg
Lane 4: Hep-G2 whole cell lysate at 10 µg
Lane 5: A-549 whole cell lysate at 10 µg
Lane 6: SW620 whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 168 kDa
Observed band size: 220 kDa
Exposure time: 30s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (Anti-BCKDHA (phospho S292) antibody [BLR060F] ab275961) at 1/1000 dilution
Lane 1: HEK293T cells mock treated (-) whole cell lysate at 50 µg
Lane 2: HEK293T cells treated with phosphatases (+) whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 49 kDa
Exposure time: 3min
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (Anti-hnRNP M3-M4 antibody [2A6-2H3] ab128143) at 1/1000 dilution
Lane 1: HEK293T whole cell lysate at 5 µg
Lane 2: LNCaP whole cell lysate at 5 µg
Lane 3: A-549 whole cell lysate at 5 µg
Lane 4: MCF-7 whole cell lysate at 5 µg
Lane 5: K-562 whole cell lysate at 5 µg
Secondary
All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (Goat Anti-Mouse IgG H&L (HRP) ab97023)
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 80 kDa
Exposure time: 3s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-TRAP220/MED1 antibody [BLR037F] (Anti-TRAP220/MED1 antibody [BLR037F] ab243893) at 1/1000 dilution
Lane 1: EL4 whole cell lysate at 10 µg
Lane 2: mIMCD-3 whole cell lysate at 10 µg
Lane 3: RenCa whole cell lysate at 10 µg
Lane 4: NIH 3T3 whole cell lysate at 10 µg
Lane 5: TCMK-1 whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 167 kDa
Observed band size: 220 kDa
Exposure time: 30s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-CTCF antibody [BLR041F] (Anti-CTCF antibody [BLR041F] ab243897) at 1/1000 dilution
Lane 1: HEK293T whole cell lysate at 50 µg
Lane 2: MCF-7 whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: Hep-G2 whole cell lysate at 50 µg
Lane 5: A-549 whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 83 kDa
Observed band size: 127 kDa
Exposure time: 3s
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)
All lanes: Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (Anti-BCKDHA (phospho S292) antibody [BLR060F] ab275961) at 1/1000 dilution
Lane 1: NIH 3T3 whole cell lysate at 50 µg
Lane 2: CT26 whole cell lysate at 50 µg
Lane 3: TCMK-1 whole cell lysate at 50 µg
Lane 4: BW5147.3 whole cell lysate at 50 µg
Lane 5: CH27 whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 49 kDa
Exposure time: 75s