Rabbit Polyclonal beta Actin antibody. Cytoskeleton marker. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 89 publications.
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- Cell communication and signaling : CCS 21:1202023Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKlara Hanelova,Martina Raudenska,Monika Kratochvilova,Jiri Navratil,Tomas Vicar,Maria Bugajova,Jaromir Gumulec,Michal Masarik,Jan BalvanPubMed 37226246
- Iranian journal of basic medical sciences 25:1009-10152022Effects of different-intensity exercise and creatine supplementation on mitochondrial biogenesis and redox status in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeyhan Taskin,Taskin Celik,Seniz Demiryurek,Sibel Turedi,Abdullah TaskinPubMed 36159328
- Shock (Augusta, Ga.) 58:400-4072022MILD TRAUMATIC BRAIN INJURY ATTENUATES PNEUMONIA-INDUCED LUNG INJURY BY MODULATIONS OF ALVEOLAR MACROPHAGE BACTERICIDAL ACTIVITY AND M1 POLARIZATION.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Ruan,Jing Chen,Jianxin Yang,Guirong WangPubMed 36166827
- Turkish journal of medical sciences 51:3126-31352021Neuropeptide-S affects cognitive impairment and depression-like behavior on MPTP induced experimental mouse model of Parkinson’s diseaseApplications:Unspecified applicationReactive species:Unspecified reactive speciesAyşe Özkan,Mehmet Bülbül,Narin Derin,Osman Sinen,Güven Akçay,Hande Parlak,Mutay Aydın Aslan,Aysel AğarPubMed 34289654
- Experimental neurobiology 30:341-3552021Overexpression of SIRT3 Suppresses Oxidative Stress-induced Neurotoxicity and Mitochondrial Dysfunction in Dopaminergic Neuronal Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShinrye Lee,Yu-Mi Jeon,Myungjin Jo,Hyung-Jun KimPubMed 34737239
- Cancer reports (Hoboken, N.J.) 5:e14852021A novel polyethylene glycol (PEG)-drug conjugate of Venetoclax, a Bcl-2 inhibitor, for treatment of acute myeloid leukemia (AML).Applications:Unspecified applicationReactive species:Unspecified reactive speciesHidenori Ando,Yuta Murakami,Kiyoshi Eshima,Tatsuhiro IshidaPubMed 34173723
- Biological & pharmaceutical bulletin 44:844-8522021Increasing Tumor Extracellular pH by an Oral Alkalinizing Agent Improves Antitumor Responses of Anti-PD-1 Antibody: Implication of Relationships between Serum Bicarbonate Concentrations, Urinary pH, and Therapeutic Outcomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHidenori Ando,Sherif E Emam,Yoshino Kawaguchi,Taro Shimizu,Yu Ishima,Kiyoshi Eshima,Tatsuhiro IshidaPubMed 34078817
- Experimental and therapeutic medicine 21:5962021Microcirculatory disturbance in acute liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkifumi Kuwano,Miho Kurokawa,Motoyuki Kohjima,Koji Imoto,Shigeki Tashiro,Hideo Suzuki,Masatake Tanaka,Seiji Okada,Masaki Kato,Yoshihiro OgawaPubMed 33884034
- International journal of medical sciences 18:1778-17852021effects of velvet antler water extracts from Formosan Sambar deer and red deer on barrier integrity in Caco-2 cell.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Kai Hung,Shang-Tse Ho,Ching-Yun Kuo,Ming-Ju ChenPubMed 33746595
- International journal of medical sciences 18:207-2152021Reduction of pl-CSA through ChSy-2 knockout inhibits tumorigenesis and metastasis of choriocarcinoma in JEG3 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuzuo Zhang et. al.PubMed 33390789
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
Beta-actin, ACTB
Recommended products
Rabbit Polyclonal beta Actin antibody. Cytoskeleton marker. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 89 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
- Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
- Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
- Associated diseases and disorders
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
All lanes: Western blot - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039) at 1 µg/mL
Lane 1: SK-N-SH (human neuroblastoma) whole cell lysate at 10 µg
Lane 2: Human spleen tissue lysate - total protein (ab29699) at 10 µg
Lane 3: U-2 OS (human osteosarcoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 41 kDa
Observed band size: 42 kDa
Immunoprecipitation - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
beta Actin was immunoprecipitated using 0.5mg SK-N-SH (human neuroblastoma cell line) whole cell extract, 5 μg of Rabbit polyclonal to beta Actin and 50 μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10 minutes, SK-N-SH whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.
Proteins were eluted by addition of 40 μl SDS loading buffer and incubated for 10 minutes at 70°C; 10 μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16039.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 42kDa; beta Actin
All lanes: Immunoprecipitation - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Exposure time: 20min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
Formalin-fixed, paraffin-embedded human leiomyoma tissue stained for beta Actin with ab16039 (1/100 dilution) in immunohistochemical analysis.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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