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Rabbit Recombinant Monoclonal beta Actin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 15 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21241] (AB213262), expandable thumbnail
  • Western blot - Anti-beta Actin antibody [EPR21241] (AB213262), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21241] (AB213262), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Rabbit
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Tested
Cow
Predicted
Predicted
Dog
Predicted
Predicted
Pig
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Human
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Cow, Dog, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.05000-0.50000 µg/mL
Notes

-

Species
Human
Dilution info
0.05000-0.50000 µg/mL
Notes

-

Predicted
Predicted

Species
Cow, Dog, Pig
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

Alternative names

Beta-actin, ACTB

Recommended products

Rabbit Recombinant Monoclonal beta Actin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 15 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21241
Purification technique
Affinity purification Protein A
Specificity

In-house testing showed ab213262 to react with gamma actin in western blot.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

If this antibody is used as a loading control in WB and if the signal of the gamma actin is close to the protein of interest to be detected, we recommend cutting the membrane between the expected signals of beta actin and gamma actin and to stain the two parts containing the loading control and the protein of interest separately.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Associated diseases and disorders

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21241] (ab213262), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21241] (ab213262)

    Anti-Calnexin antibody [EPR21205] ab213243 staining beta-Actin in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213262 at 0.5ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-beta Actin antibody [EPR21241] (ab213262), expandable thumbnail

    Western blot - Anti-beta Actin antibody [EPR21241] (ab213262)

    ab213262 was shown to react with ACTNB (beta actin) when tested against HAP1 wild-type and HAP1 ACTNB (beta actin) knockout cells. ACTNB wild-type and knockout samples were subjected to SDS-PAGE and incubated with ab213262. Incomplete reduction of signal was seen in the ACTNB (beta actin) knockout samples at beta actin's expected molecular weight. SDS-PAGE of gamma actin recombinant protein and incubation with ab213262 showed cross-reactivity to gamma actin.

    ab213262 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-beta Actin antibody [EPR21241] (ab213262)

    Lane 1: HAP1 whole cell lysate at 20 µg

    Lane 2: HAP1 ACTNB knockout whole cell lysate at 20 µg

    Lane 3: MEF1 whole cell lysate at 20 µg

    Lane 4: NIH3T3 whole cell lysate at 20 µg

    Lane 5: Western blot - Recombinant Human gamma Actin protein (Recombinant Human gamma Actin protein ab157841) at 0.1 µg

    Performed under reducing conditions.

    Predicted band size: 41 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21241] (ab213262), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21241] (ab213262)

    Anti-Calnexin antibody [EPR21205] ab213243 staining beta-Actin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213262 at 0.01ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

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