AB210083

Anti-beta Actin antibody - Loading Control

Lab Essentials
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(4 Publications)

Rabbit Polyclonal ACTB1 antibody. Suitable for IHC-P, WB and reacts with Zebrafish samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Zebrafish actba aa 300 to C-terminus.

View Alternative Names

bact, bactin1, bactzf, actba, Beta-actin-1

2 Images

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB210083)

10% SDS-PAGE

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab210083) at 1/1000 dilution

Lane 1:

zebrafish heart tissue extract at 30 µg

Lane 2:

zebrafish muscle tissue extract at 30 µg

Lane 3:

whole zebrafish tissue extract at 30 µg

Predicted band size: 41 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Loading Control (AB210083)

Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling beta Actin with ab210083 at 1/300 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Zebrafish actba aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7ZVI7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

See full target information actba

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6261 PubMed40623999

2025

YY1 enhances HIF-1α stability in tumor-associated macrophages to suppress anti-tumor immunity of prostate cancer in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wenchao Li,SaiSai Chen,Jian Lu,Weipu Mao,Shiya Zheng,Minhao Zhang,Tiange Wu,Yurui Chen,Kai Lu,Chunyan Chu,Chuanjun Shu,Yue Hou,Xue Yang,Naipeng Shi,Zhijun Chen,Lihua Zhang,Lei Zhang,Rong Na,Ming Chen,Shenghong Ju,Dingxiao Zhang,Yi Ma,Bin Xu

Scientific reports 15:1787 PubMed39805936

2025

GNG2 inhibits brain metastases from colorectal cancer via PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chenhua Luo,ZhiMing Xiao,WenLong Yang

Fluids and barriers of the CNS 21:8 PubMed38243347

2024

Oxygen glucose deprivation-pretreated astrocyte-derived exosomes attenuates intracerebral hemorrhage (ICH)-induced BBB disruption through miR-27a-3p /ARHGAP25/Wnt/β-catenin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Hou,Ye Xie,Xiaoxuan Liu,Yushan Chen,Fangfang Zhou,Binbin Yang

Neurochemical research 46:1239-1251 PubMed33646533

2021

Benefits of Iron Chelators in the Treatment of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Zeng,Hedi An,Fei Yu,Kai Wang,Lanlan Zheng,Wei Zhou,Yiwen Bao,Jie Yang,Nan Shen,Dongya Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Actin antibody - Loading Control

Western blot - Anti-beta Actin antibody - Loading Control (AB210083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Loading Control (AB210083)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise