Rabbit Polyclonal beta Actin antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Cited in 129 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTB.
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- BMC cancer 23:10532023Anti-proliferative activity of RIHMS-Qi-23 against MCF-7 breast cancer cell line is through inhibition of cell proliferation and senescence but not inhibition of targeted kinases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRanda El-Gamal,Sara Elfarrash,Mohammad El-Nablaway,Asmaa Ahmed Salem,Seyed-Omar Zaraei,Hanan S Anbar,Ashraf Shoma,Mohammed I El-GamalPubMed 37919708
- Cells 12:2023The Mechanoreception in Oocyte under Modeling Micro- and Hypergravity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrina V OgnevaPubMed 37508484
- International archives of allergy and immunology 184:1056-10702023CircARF3 Mitigates Allergic Rhinitis through Targeting microRNA-205-5p/Sirtuin 5 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zheng,Xi Chen,Jia-Bin Zhan,Chang-Wu Li,Xin Wei,Hong-Yan JiangPubMed 37343537
- Phytotherapy research : PTR 37:4149-4165202311-Ethoxyviburtinal improves chronic restraint stress-induced anxiety-like behaviors in gender-specific mice via PI3K/Akt and E /ERβ signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Lyu,Xiaojia Wei,Xue Yang,Jiayuan Li,Guohui Wan,Yuqing Wang,Zhuangzhuang Hao,Yifan Lu,Jianyou Guo,Jinli ShiPubMed 37300355
- Molecular biology reports 50:5755-57652023The size of human subcutaneous adipocytes, but not adiposity, is associated with inflammation, endoplasmic reticulum stress, and insulin resistance markers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Pourdashti,Nassim Faridi,Forouzandeh Monem-Homaie,S Hamid Yaghooti,Ahmadreza Soroush,S Zahra BathaiePubMed 37219669
- Journal of biochemical and molecular toxicology 37:e233832023Knockdown of circMFN2 inhibits cell progression and glycolysis by miR-198/CUL4B pathway in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Song,Ting Chai,Junqi Liu,Alan Chu,Chen Sun,Zongwen LiuPubMed 37158446
- Open medicine (Warsaw, Poland) 18:202306652023HDAC5-mediated PRAME regulates the proliferation, migration, invasion, and EMT of laryngeal squamous cell carcinoma via the PI3K/AKT/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Yu,Huan Cao,Jian-Wang Yang,Wen-Xia Meng,Chuan Yang,Jian-Tao Wang,Miao-Miao Yu,Bao-Shan WangPubMed 36910848
- Cells 12:2023Inhibition of Abl Kinase by Imatinib Can Rescue the Compromised Barrier Function of 22q11.2DS Patient-iPSC-Derived Blood-Brain Barriers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunfei Li,Zhixiong Sun,Huixiang Zhu,Yan Sun,David B Shteyman,Sander Markx,Kam W Leong,Bin Xu,Bingmei M FuPubMed 36766762
- Cells 11:2022Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrina V Ogneva,Maria A Golubkova,Nikolay S Biryukov,Oleg V KotovPubMed 36497128
- Nature communications 13:70742022Centromere defects, chromosome instability, and cGAS-STING activation in systemic sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSouren Paul,Mark H Kaplan,Dinesh Khanna,Preston M McCourt,Anjan K Saha,Pei-Suen Tsou,Mahek Anand,Alexander Radecki,Mohamad Mourad,Amr H Sawalha,David M Markovitz,Rafael Contreras-GalindoPubMed 36400785
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ACTB. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
Consider this alternative
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Expected | Tested | Tested | Expected |
| Rat | Expected | Tested | Tested | Expected |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted |
| Drosophila melanogaster | Expected | Tested | Expected | Expected |
| Guinea pig | Predicted | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 - 1/20000 | Notes - |
Species Rat | Dilution info 1/5000 - 1/20000 | Notes - |
Species Drosophila melanogaster | Dilution info 1/5000 - 1/20000 | Notes - |
Species Human | Dilution info 1/5000 - 1/20000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
Beta-actin, ACTB
Recommended products
Rabbit Polyclonal beta Actin antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Cited in 129 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTB.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ACTB. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
- Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
- Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
- Associated diseases and disorders
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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13 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
Paraffin-embedded mouse pancreas tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab227387)
0.5% Triton X-100 followed by ice-cold methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab227387 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Western blot - Anti-beta Actin antibody - Loading Control (ab227387)
Beta Actin Western blot staining using rabbit Anti-beta Actin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Lane 5: Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg
Lane 6: C8D30 whole cell extract at 30 µg
Lane 7: NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg
Lane 8: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
Lane 9: C2C12 (mouse myoblast cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
Paraffin-embedded rat liver tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-beta Actin antibody - Loading Control (ab227387)
Beta Actin Western blot staining using rabbit Anti-beta Actin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa
Western blot - Anti-beta Actin antibody - Loading Control (ab227387)
Beta Actin Western blot staining using rabbit Anti-beta Actin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/10000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 15 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 5 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 2 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa
Immunoprecipitation - Anti-beta Actin antibody - Loading Control (ab227387)
beta Actin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg of ab227387. Western blot was performed from the immunoprecipitate using ab227387. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab227387 in HEK-293T whole cell extract.
Lane 3: ab227387 IP in HEK-293T whole cell extract.
All lanes: Immunoprecipitation - Anti-beta Actin antibody - Loading Control (ab227387)
Predicted band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
Paraffin-embedded mouse cervix tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-beta Actin antibody - Loading Control (ab227387)
Beta Actin Western blot staining of Drosophila melanogaster lysate using rabbit Anti-beta Actin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/10000 dilution
All lanes: Drosophila melanogaster lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa
Western blot - Anti-beta Actin antibody - Loading Control (ab227387)
Beta Actin Western blot staining using rabbit Anti-beta Actin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/15000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 10 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with beta Actin shRNA, whole cell extract at 10 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa
Western blot - Anti-beta Actin antibody - Loading Control (ab227387)
Beta Actin Western blot staining using rabbit Anti-beta Actin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa
Western blot - Anti-beta Actin antibody - Loading Control (ab227387)
Beta Actin Western blot staining of Mouse liver tissue extract using rabbit Anti-beta Actin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution
All lanes: Mouse liver tissue extract at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa
Western blot - Anti-beta Actin antibody - Loading Control (ab227387)
Beta Actin Western blot staining of Rat liver tissue extract using rabbit Anti-beta Actin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution
All lanes: Rat liver tissue extract at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 41 kDa
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