AB227387

Anti-beta Actin antibody - Loading Control

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(171 Publications)

Rabbit Polyclonal beta Actin antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Cited in 171 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTB.

View Alternative Names

Beta-actin, ACTB

16 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody - Loading Control (AB227387)

0.5% Triton X-100 followed by ice-cold methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab227387 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Supplier Data

Immunoprecipitation - Anti-beta Actin antibody - Loading Control (AB227387)

beta Actin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg of ab227387. Western blot was performed from the immunoprecipitate using ab227387. Anti-rabbit IgG was used as secondary antibody.

Lane 1 : HEK-293T whole cell extract (Input).

Lane 2 : Control IgG instead of ab227387 in HEK-293T whole cell extract.

Lane 3 : ab227387 IP in HEK-293T whole cell extract.

All lanes:

Immunoprecipitation - Anti-beta Actin antibody - Loading Control (ab227387)

Predicted band size: 41 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Loading Control (AB227387)

Paraffin-embedded mouse cervix tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Loading Control (AB227387)

Paraffin-embedded mouse pancreas tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Loading Control (AB227387)

Paraffin-embedded rat liver tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/10000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 5 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 2 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 5:

Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 6:

C8D30 whole cell extract at 30 µg

Lane 7:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg

Lane 8:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Lane 9:

C2C12 (mouse myoblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/10000 dilution

Lane 1:

293T whole cell extracts at 5 µg

Lane 2:

293T whole cell extracts at 2 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/1000 dilution

Lane 1:

Mouse testis tissue extracts at 50 µg

Lane 2:

Mouse liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution

All lanes:

Rat liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/10000 dilution

Lane 1:

Neuro2a whole cell lysates at 30 µg

Lane 2:

Rat2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/20000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/10000 dilution

All lanes:

Drosophila melanogaster lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (ab227387) at 1/15000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 10 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with beta Actin shRNA, whole cell extract at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Drosophila melanogaster

Applications

IP, ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACTB. The exact immunogen used to generate this antibody is proprietary information.

P60709

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/1000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information ACTB

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Publications (171)

Recent publications for all applications. Explore the full list and refine your search

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Unspecified application

Species

Unspecified reactive species

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Investigation of the Protective Effect of Red-Light-Intervened Dendrobium officinale on Rats With Gastric Injury Based on Serum Metabolomics.

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Mohamed El-Sherbiny,Hany A Elkattawy,Shimaa Hadhoud,Ahmed N Nasr,Ateya M Ibrahim,Omar Z Ameer,Noorhan Alsaleebi,Joud Asfari,Madaniah O Zakari,Moaz A Mojaddidi,Ehab K Ali,Hailah M Almohaimeed,Ahmed Abdeen,Sahar K Ali,Mamdouh Eldesoqui

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Unspecified application

Species

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Species

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta Actin antibody - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody - Loading Control (AB227387)

Immunoprecipitation - Anti-beta Actin antibody - Loading Control (AB227387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Loading Control (AB227387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Loading Control (AB227387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Western blot - Anti-beta Actin antibody - Loading Control (AB227387)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (171)

Product promise