AB253283

Anti-beta Actin antibody [M008] - Loading Control

Lab Essentials
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(3 Publications)

Mouse Monoclonal beta Actin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTB.

View Alternative Names

Beta-actin, ACTB

2 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [M008] - Loading Control (AB253283)

Paraformaldehyde-fixed MeWo (human epidermoid carcinoma cell line) cells stained for beta Actin (red) using ab253283 at 1/50 dilution in ICC/IF. The antibody was detected using goat anti-mouse DyLight^® 594.

Supplier Data

Western blot - Anti-beta Actin antibody [M008] - Loading Control (AB253283)

Primary for 1 hour at room temperature in PBS/5% non-fat milk/0.045 Tween 20.

All lanes:

Western blot - Anti-beta Actin antibody [M008] - Loading Control (ab253283) at 1/1000 dilution

Lane 1:

HUVEC-CS cell lysate

Lane 2:

Rabbit spleen fibroblast cell lysate

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 4:

LNCaP (human prostate cancer cell line) cell lysate

Lane 5:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 6:

F9 (mouse embryonic testicular cancer cell line) cell lysate

Predicted band size: 41 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M008

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACTB. The exact immunogen used to generate this antibody is proprietary information.

P60709

Specificity

ab253283 preferentially detects human beta Actin with only weak reactivity toward actins in rat, mouse, or rabbit.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information ACTB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 14:34 PubMed40885723

2025

Targeting pregnane X receptor with a potent agonist-based PROTAC to delay colon cancer relapse.

Applications

Unspecified application

Species

Unspecified reactive species

Lucile Bansard,Guillaume Laconde,Vanessa Delfosse,Tiphaine Huet,Margaux Ayeul,Emilie Rigal,Quentin Donati,Sabine Gerbal-Chaloin,Martine Daujat-Chavanieu,Luc Brunel,Baptiste Legrand,Alain Chavanieu,Anthony R Martin,Julie Pannequin,William Bourguet,Muriel Amblard,Jean Marc Pascussi

Thoracic cancer 14:1969-1979 PubMed37220935

2023

Circular non-coding RNA circ_0072088 serves as a ceRNA, targeting the miR-1225-5p/WT1 axis to regulate non-small cell lung cancer cell malignant behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Zhu,Jing Wan,Xu You,Wanli Yang,Lei Zhao

Frontiers in molecular neuroscience 15:959224 PubMed36245919

2022

Repeated inhibition of sigma-1 receptor suppresses GABA receptor expression and long-term depression in the nucleus accumbens leading to depressive-like behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoyao Qin,Weixing Xu,Kunpeng Li,Qi Luo,Xi Chen,Yue Wang,Lei Chen,Sha Sha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta Actin antibody [M008] - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [M008] - Loading Control (AB253283)

Western blot - Anti-beta Actin antibody [M008] - Loading Control (AB253283)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise