AB178787

Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free

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(29 Publications)

Mouse Monoclonal beta Actin antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Schizosaccharomyces pombe, Drosophila melanogaster, Xenopus laevis samples. Cited in 29 publications.

View Alternative Names

Beta-actin, ACTB

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (AB178787)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (AB178787)

IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3μg/ml overnight at +4°C. A goat anti-mouse HRP-conjugated secondary antibody (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab8224).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (AB178787)

IHC image of ab8224 staining beta Actin in human colon formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8224, 1μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab8224).

Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (AB178787)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (AB178787)

This data was developed using the same antibody clone in a different buffer formulation containing BSA and sodium azide (ab8224). ab8224 staining beta Actin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab8224 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

HRP

HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 8224

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse, Rat, Human, Xenopus laevis, Drosophila melanogaster, Schizosaccharomyces pombe

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recognises a single band at 42kD representing beta Actin. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

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Block membrane for 1 hr in 5%BSA. Incubate antibody in TBST for one hour or more.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5%BSA. Incubate antibody in TBST for one hour or more.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5%BSA. Incubate antibody in TBST for one hour or more.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5%BSA. Incubate antibody in TBST for one hour or more.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Schizosaccharomyces pombe": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5%BSA. Incubate antibody in TBST for one hour or more.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5%BSA. Incubate antibody in TBST for one hour or more.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab178787 is a PBS Obly version of ab8224.

This clone works well as a loading control for Xenopus, Drosophila, S. cerevisiae and S.pombe. We recommend using ab8224 instead of ab8226 for these species.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information ACTB

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 53:112-122 PubMed39786883

2025

Acanthoside B attenuates NLRP3-mediated pyroptosis and ulcerative colitis through inhibition of tAGE/RAGE pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo He,Chunfang Zhou,Rui Shang,Xiaoyan Wang

iScience 27:110436 PubMed39262802

2024

SOX6 expression and aneurysms of the thoracic and abdominal aorta.

Applications

Unspecified application

Species

Unspecified reactive species

David Carmona-Berrio,Isabel Adarve-Rengifo,Andrea G Marshall,Zer Vue,Duane D Hall,Tyne W Miller-Fleming,Ky'Era V Actkins,Heather K Beasley,Paula M Almonacid,Pierina Barturen-Larrea,Quinn S Wells,Marcos G Lopez,Edgar Garza-Lopez,Dao-Fu Dai,Jianqiang Shao,Kit Neikirk,Frederic T Billings,John A Curci,Nancy J Cox,Vivian Gama,Antentor Hinton,Jose A Gomez

Cell death discovery 10:361 PubMed39138186

2024

METTL14-mediated m6A mRNA modification of G6PD promotes lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weidong Wu,Mengling Li,Yingxiao Wu,Qiongying Wei,Nanding Yu

Balkan medical journal 41:174-185 PubMed38700313

2024

Thymic Stromal Lymphopoietin Activates Mouse Dendritic Cells Through the JAK/SYK Pathway in Promoting Th17 Response in Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Cuihao Song,Jie Sun,Zhenkai Zhao,Xu Zhang,Xiangyu Ding,Xiaoqiang Liang,Jia Bai,Liyuan Xing,Lingling Gong,Chengxin Li,Biwen Lin

International journal of molecular sciences 24: PubMed37047563

2023

Berberine Induces Combined Cell Death in Gastrointestinal Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Shiori Mori,Rina Fujiwara-Tani,Momoko Gyoten,Shota Nukaga,Rika Sasaki,Ayaka Ikemoto,Ruiko Ogata,Shingo Kishi,Kiyomu Fujii,Hiroki Kuniyasu

Journal of translational medicine 11:130 PubMed23706090

2013

A proteomic view of isoproterenol induced cardiac hypertrophy: prohibitin identified as a potential biomarker in rats.

Applications

Species

Rat

Debabrata Chowdhury,Anjana Devi Tangutur,Tarak Nath Khatua,Priyanka Saxena,Sanjay K Banerjee,Manika Pal Bhadra

Molecular and cellular biology 32:2099-109 PubMed22451489

2012

Cyclin-dependent kinase 8 regulates mitotic commitment in fission yeast.

Applications

Species

Schizosaccharomyces pombe

Zsolt Szilagyi,Gabor Banyai,Marcela Davila Lopez,Christopher J McInerny,Claes M Gustafsson

Molecular biology of the cell 22:4447-59 PubMed21965290

2011

Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

R Scott McIsaac,Sanford J Silverman,Megan N McClean,Patrick A Gibney,Joanna Macinskas,Mark J Hickman,Allegra A Petti,David Botstein

PloS one 6:e21113 PubMed21698254

2011

The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes.

Applications

Species

Saccharomyces cerevisiae S288C

Monica E Ferreira,Kacie Flaherty,Philippe Prochasson

Journal of cell science 124:1844-56 PubMed21558413

2011

In vivo functional analysis reveals specific roles for the integrin-binding sites of talin.

Applications

Species

Unspecified reactive species

Stephanie J Ellis,Mary Pines,Michael J Fairchild,Guy Tanentzapf

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (AB178787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (AB178787)

Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (AB178787)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (29)

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