Skip to main content

Mouse Monoclonal beta Actin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 3364 publications.

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Tested
Tested
Tested
African green monkey
Predicted
Predicted
Predicted
Armenian hamster
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Chinese hamster
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Guinea pig
Predicted
Predicted
Predicted
Horse
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted

Images

Tested
Tested

Species
Rat
Dilution info
0.10000-0.50000 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species
Human
Dilution info
0.10000-0.50000 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Monkey, Zebrafish, African green monkey, Chinese hamster, Armenian hamster
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1 µg/mL
Notes

We recommend blocking using 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please refer to the images section for the blocking comparison data.

Species
Rat
Dilution info
1 µg/mL
Notes

We recommend blocking using 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please refer to the images section for the blocking comparison data.

Species
Human
Dilution info
1 µg/mL
Notes

We recommend blocking using 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please refer to the images section for the blocking comparison data.

Predicted
Predicted

Species
Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Monkey, Zebrafish, African green monkey, Chinese hamster, Armenian hamster
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
5 µg/mL
Notes

-

Species
Human
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Monkey, Zebrafish, African green monkey, Chinese hamster, Armenian hamster
Dilution info
-
Notes

-

Target data

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Mouse Monoclonal beta Actin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 3364 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

mAbcam 8226

Purification technique

Affinity purification Protein G

Specificity

Does not cross-react with adult cardiac, smooth, or skeletal muscle actin. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally.

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Western blot protocol advice:

We recommend blocking with 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin. Please see the comparison data in the images section. If milk block is required, we recommend using ab8224 mouse monoclonal [mAbcam 8224] to beta actin. Contact our Scientific Support team for more information or advice.

This antibody clone [mAbcam 8226] is manufactured by Abcam.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com