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Rabbit Polyclonal ACTB1 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Zebrafish samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Zebrafish actba aa 1-100.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - N-terminal (AB209869), expandable thumbnail
  • Western blot - Anti-beta Actin antibody - N-terminal (AB209869), expandable thumbnail
  • Western blot - Anti-beta Actin antibody - N-terminal (AB209869), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Zebrafish actba aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q7ZVI7

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Zebrafish
Tested
Tested

Tested
Tested

Species

Zebrafish

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Zebrafish

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

Alternative names

Recommended products

Rabbit Polyclonal ACTB1 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Zebrafish samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Zebrafish actba aa 1-100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Zebrafish actba aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q7ZVI7
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Associated diseases and disorders

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - N-terminal (ab209869), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - N-terminal (ab209869)

    Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling beta Actin with ab209869 at 1/300 dilution.

  • Western blot - Anti-beta Actin antibody - N-terminal (ab209869), expandable thumbnail

    Western blot - Anti-beta Actin antibody - N-terminal (ab209869)

    10% SDS-PAGE

    All lanes: Western blot - Anti-beta Actin antibody - N-terminal (ab209869) at 1/500 dilution

    Lane 1: Zebrafish heart tissue extract at 30 µg

    Lane 2: Whole Zebrafish tissue extract at 30 µg

    Predicted band size: 41 kDa

  • Western blot - Anti-beta Actin antibody - N-terminal (ab209869), expandable thumbnail

    Western blot - Anti-beta Actin antibody - N-terminal (ab209869)

    10 % SDS-PAGE

    All lanes: Western blot - Anti-beta Actin antibody - N-terminal (ab209869) at 1/1000 dilution

    Lane 1: Zebrafish eye tissue extract at 20 µg

    Lane 2: Zebrafish brain tissue extract at 20 µg

    Lane 3: Zebrafish ovary tissue extract at 20 µg

    Lane 4: Zebrafish liver tissue extract at 20 µg

    Predicted band size: 41 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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