AB209869

Anti-beta Actin antibody - N-terminal - Loading Control

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(4 Publications)

Rabbit Polyclonal ACTB1 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Zebrafish samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Zebrafish actba aa 1-100.

View Alternative Names

bact, bactin1, bactzf, actba, Beta-actin-1

3 Images

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - N-terminal - Loading Control (AB209869)

Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling beta Actin with ab209869 at 1/300 dilution.

Supplier Data

Western blot - Anti-beta Actin antibody - N-terminal - Loading Control (AB209869)

10 % SDS-PAGE

All lanes:

Western blot - Anti-beta Actin antibody - N-terminal - Loading Control (ab209869) at 1/1000 dilution

Lane 1:

Zebrafish eye tissue extract at 20 µg

Lane 2:

Zebrafish brain tissue extract at 20 µg

Lane 3:

Zebrafish ovary tissue extract at 20 µg

Lane 4:

Zebrafish liver tissue extract at 20 µg

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-beta Actin antibody - N-terminal - Loading Control (AB209869)

10% SDS-PAGE

All lanes:

Western blot - Anti-beta Actin antibody - N-terminal - Loading Control (ab209869) at 1/500 dilution

Lane 1:

Zebrafish heart tissue extract at 30 µg

Lane 2:

Whole Zebrafish tissue extract at 30 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Zebrafish actba aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q7ZVI7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

See full target information actba

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 11: PubMed36009286

2022

Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Suzan Attia Mawed,Carlotta Marini,Mahmoud Alagawany,Mayada R Farag,Rasha M Reda,Mohamed T El-Saadony,Walaa M Elhady,Gian E Magi,Alessandro Di Cerbo,Wafaa G El-Nagar

Frontiers in molecular neuroscience 14:723912 PubMed34630034

2021

Chip Protein U-Box Domain Truncation Affects Purkinje Neuron Morphology and Leads to Behavioral Changes in Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Yasaman Pakdaman,Elsa Denker,Eirik Austad,William H J Norton,Hans O Rolfsnes,Laurence A Bindoff,Charalampos Tzoulis,Ingvild Aukrust,Per M Knappskog,Stefan Johansson,Ståle Ellingsen

The International journal of developmental biology 65:513-522 PubMed34549797

2021

Maternal and zygotic activin signaling promotes adequate pattern and differentiation of mesoderm through regulation of pluripotency genes during zebrafish development.

Applications

Unspecified application

Species

Unspecified reactive species

Shaghayegh Hasanpour,Soheil Eagderi,Hadi Poorbagher,Mohammad Hasanpour

Proceedings of the National Academy of Sciences of 117:15799-15808 PubMed32571908

2020

Pervasive changes of mRNA splicing in -deficient zebrafish identify as a regulator of T cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Divine-Fondzenyuy Lawir,Katarzyna Sikora,Connor P O'Meara,Michael Schorpp,Thomas Boehm

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Actin antibody - N-terminal - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - N-terminal - Loading Control (AB209869)

Western blot - Anti-beta Actin antibody - N-terminal - Loading Control (AB209869)

Western blot - Anti-beta Actin antibody - N-terminal - Loading Control (AB209869)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise