Anti-beta Actin (mono methyl H73) antibody [EPR29292-70]

12 product images

• 

  Western blot - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Lanes 1-6 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lanes 7-8 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

  Exposure time: Lanes 1-7: 81 seconds; Lane 8: 180 seconds

  All lanes: Western blot - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203) at 1/1000 dilution

  Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

  Lane 3: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 4: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

  Lane 5: Mouse brain tissue lysate at 20 µg

  Lane 6: Mouse spleen tissue lysate at 20 µg

  Lane 7: Human spleen tissue lysate at 20 µg

  Lane 8: Human liver tissue lysate at 20 µg

  Secondary

  Lanes 1 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Lanes 7 - 8: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 41 kDa

• 

  Dr Hai-Ning Du's lab, Wuhan University

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Immunohistochemical analysis of paraffin-embedded (A) Wild-type mouse skeletal muscle. (B) SETD3 knockout mouse skeletal muscle. tissue labeling beta Actin (mono methyl H73) with ab322203 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in (A) Wild-type mouse skeletal muscle, no staining in (B) SETD3 knockout mouse skeletal muscle.
  
  The Wild-type and SETD3 knockout mouse skeletal muscle sections were kindly provided by Dr Hai-Ning Du's lab, Wuhan University.
  
  The section was incubated with ab322203 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Western blot - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  All lanes: Western blot - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203) at 1/1000 dilution

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 41 kDa

  Exposure time: 81s

• 

  Western blot - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  SETD3 is an actin specific histidine methyltransferase that catalyzes the Nτ -methylation reaction of histidine 73 (H73) residue in human actin.

  The Wild-type PC-9 and SETD3 knock out PC-9 lysates were kindly provided by Dr Hai-Ning Du's lab, Wuhan University.

  All lanes: Western blot - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203) at 1/1000 dilution

  Lane 1: Wild-type PC-9 (human lung adenocarcinoma cell) whole cell lysate at 20 µg

  Lane 2: SETD3 knock out PC-9 whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 41 kDa

  Exposure time: 81s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling beta Actin (mono methyl H73) with ab322203 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in mouse spleen.
  
  The section was incubated with ab322203 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Immunohistochemical analysis of paraffin-embedded Mouse lung tumor tissue labeling beta Actin (mono methyl H73) with ab322203 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in mouse lung tumor.
  
  The section was incubated with ab322203 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling beta Actin (mono methyl H73) with ab322203 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in mouse kidney.
  
  The section was incubated with ab322203 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling beta Actin (mono methyl H73) with ab322203 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in human mammary gland.
  
  The section was incubated with ab322203 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling beta Actin (mono methyl H73) with ab322203 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in human lung carcinoma.
  
  The section was incubated with ab322203 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling beta Actin (mono methyl H73) with ab322203 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in immune cell of human liver.
  
  The section was incubated with ab322203 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling beta Actin (mono methyl H73) with ab322203 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in human kidney.
  
  The section was incubated with ab322203 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin (mono methyl H73) antibody [EPR29292-70] (ab322203)

  Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling beta Actin (mono methyl H73) with ab322203 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in stromal cell of human endometrial carcinoma.
  
  The section was incubated with ab322203 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins