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AB254345

Anti-beta Amyloid 1-40 antibody [EPR23712-2]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(2 Reviews)

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(1 Publication)

Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345) is a rabbit monoclonal antibody detecting beta Amyloid 1-40 in Western Blot, IHC-P, ELISA, Dot Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

A4, AD1, APP, Amyloid-beta precursor protein, ABPP, APPI, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta (A4) precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, CVAP, PN-II

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)

Immunohistochemical analysis of paraffin-embedded Human Alzheimer's cerebrum tissue labelling beta-Amyloid 1-40 with ab254345 at 1/5000 dilution, followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Plaque staining on the human Alzheimer's cerebrum (PMID : 10912918). The section was incubated with ab254345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling beta-Amyloid 1-40 with ab254345 at 1/5000 dilutiob, followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Negative control : no staining on human cerebrum. The section was incubated with ab254345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)

Indirect ELISA using ab254345 at 0 - 1000 ng/mL.

Antigen concentration is 1000 ng/mL.

Secondary antibody is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)
  • WB

Lab

Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The band over 75 KD is the BSA-crosslinked Aß40 peptide.

Exposure time : 48 seconds

All lanes:

Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345) at 1/1000 dilution

Lane 1:

BSA-crosslinked beta-Amyloid (Aβ42) 1-42 peptide 20 ng

Lane 2:

BSA-crosslinked beta-Amyloid (Aβ40) 1-40 peptide 20 ng

Lane 3:

BSA-crosslinked beta-Amyloid (Aβ39) 1-39 peptide 20 ng

Lane 4:

BSA-crosslinked beta-Amyloid (Aβ38) 1-38 peptide 20 ng

Lane 5:

BSA-crosslinked beta-Amyloid (Aβ37) 1-37 peptide 20 ng

Lane 6:

BSA-crosslinked beta-Amyloid (Aβ36) 1-36 peptide 20 ng

Lane 7:

BSA-crosslinked beta-Amyloid (Aβ16) 1-16 peptide 20 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 86 kDa

Observed band size: 4 kDa,86 kDa

false

Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)
  • Dot

Lab

Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345)

Dot blot analysis of beta-Amyloid 1-40 using ab254345 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

The samples are :

Lane 1 : Beta-Amyloid (Aβ42) 1-42 peptide

Lane 2 : Beta-Amyloid (Aβ40) 1-40 peptide

Lane 3 : Beta-Amyloid (Aβ39) 1-39 peptide

Lane 4 : Beta-Amyloid (Aβ38) 1-38 peptide

Lane 5 : Beta-Amyloid (Aβ37) 1-37 peptide

Lane 6 : Beta-Amyloid (Aβ36) 1-36 peptide

Lane 7 : Beta-Amyloid (Aβ16) 1-16 peptide

Exposure time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST

  • Carrier free

    Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23712-2

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, IHC-P, WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Biochemicals

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Use at 62.5 ng/ml</p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), ELISA, Dot Blot in Human samples.

What is the molecular weight of beta Amyloid 1-40?
Anti-beta Amyloid 1-40 [EPR23712-2] (ab254345) specifically detects a band for beta Amyloid 1-40 (UniProt: P05067) at a molecular weight of 86kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR23712-2] also available for your convenience: ab254345, Carrier free - ab289991

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Amyloid 1-40 also known as amyloid beta 40 is a peptide predominantly composed of 40 amino acids and it is a major product of amyloid precursor protein (APP) cleavage. Its molecular mass is approximately 4.3 kDa. This peptide is expressed in the brain tissue and cerebrospinal fluid. Beta amyloid engages in forming amyloid plaques which are deposits found most typically in the brain of patients with neurodegenerative conditions.
Biological function summary

Small soluble oligomers of beta amyloid interact with neuronal cells leading to synaptic dysfunction and cell death. Beta amyloid 1-40 molecules do not normally function in isolation; they often assemble into beta amyloid plaques a complex structure that can contribute to cellular anomalies. Amyloid beta production and its aggregation play parts in a process that disrupts neural communication impacting memory and learning processes.

Pathways

The amyloidogenic pathway involves beta amyloid where amyloid precursor protein (APP) is cleaved by beta-secretase and gamma-secretase to generate beta amyloid peptides including amyloid beta 40. This molecule is associated with the oxidation and inflammation pathways where it can interact with other proteins like presenilin. Presenilin is a part of the gamma-secretase complex that influences amyloid production thereby affecting the pathological aggregation process.

Beta amyloid 1-40 is closely linked to Alzheimer's disease a condition marked by cognitive decline and neural loss. The presence of amyloid plaques which are high in amyloid beta content is a characteristic marker of this disease. Furthermore beta amyloid is also associated with cerebral amyloid angiopathy a disorder where the peptide accumulates in the walls of the cerebral blood vessels. In this context tau protein another Alzheimer's-associated protein shows pathological changes influenced by the beta amyloid-induced plaque formation and contributes to neurofibrillary tangles formation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis (PubMed : 25122912). Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(o) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapses in axons (PubMed : 17062754, PubMed : 23011729). Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.. Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Amyloid-beta peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts.. Amyloid-beta protein 42. More effective reductant than amyloid-beta protein 40. May activate mononuclear phagocytes in the brain and elicit inflammatory responses.. Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain.. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.
See full target information APP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401945 PubMed38935046

2024

PICALM Regulating the Generation of Amyloid β-Peptide to Promote Anthracycline-Induced Cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Mengni Bao,Xiumeng Hua,Xiao Chen,Tao An,Han Mo,Zhe Sun,Menghao Tao,Guangxin Yue,Jiangping Song
View all publications
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Product promise

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