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Rabbit Recombinant Monoclonal Amyloid-beta precursor protein antibody. Suitable for I-ELISA, Dot, WB, IHC-P and reacts with Human samples.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISADotWBIHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Use at 62.5 ng/ml

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis (PubMed:25122912). Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapes in axons (PubMed:17062754, PubMed:23011729). Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Amyloid-beta protein 42 is a more effective reductant than amyloid-beta protein 40. Amyloid-beta peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. APP42-beta may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts.Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain.The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).

Alternative names

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Rabbit Recombinant Monoclonal Amyloid-beta precursor protein antibody. Suitable for I-ELISA, Dot, WB, IHC-P and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23712-2

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

Small soluble oligomers of beta amyloid interact with neuronal cells leading to synaptic dysfunction and cell death. Beta amyloid 1-40 molecules do not normally function in isolation; they often assemble into beta amyloid plaques a complex structure that can contribute to cellular anomalies. Amyloid beta production and its aggregation play parts in a process that disrupts neural communication impacting memory and learning processes.

Activity summary

Beta Amyloid 1-40 also known as amyloid beta 40 is a peptide predominantly composed of 40 amino acids and it is a major product of amyloid precursor protein (APP) cleavage. Its molecular mass is approximately 4.3 kDa. This peptide is expressed in the brain tissue and cerebrospinal fluid. Beta amyloid engages in forming amyloid plaques which are deposits found most typically in the brain of patients with neurodegenerative conditions.

Pathways

The amyloidogenic pathway involves beta amyloid where amyloid precursor protein (APP) is cleaved by beta-secretase and gamma-secretase to generate beta amyloid peptides including amyloid beta 40. This molecule is associated with the oxidation and inflammation pathways where it can interact with other proteins like presenilin. Presenilin is a part of the gamma-secretase complex that influences amyloid production thereby affecting the pathological aggregation process.

Associated diseases and disorders

Beta amyloid 1-40 is closely linked to Alzheimer's disease a condition marked by cognitive decline and neural loss. The presence of amyloid plaques which are high in amyloid beta content is a characteristic marker of this disease. Furthermore beta amyloid is also associated with cerebral amyloid angiopathy a disorder where the peptide accumulates in the walls of the cerebral blood vessels. In this context tau protein another Alzheimer's-associated protein shows pathological changes influenced by the beta amyloid-induced plaque formation and contributes to neurofibrillary tangles formation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345)

    Immunohistochemical analysis of paraffin-embedded Human Alzheimer's cerebrum tissue labelling beta-Amyloid 1-40 with ab254345 at 1/5000 dilution, followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Plaque staining on the human Alzheimer's cerebrum (PMID: 10912918). The section was incubated with ab254345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345), expandable thumbnail

    Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The band over 75 KD is the BSA-crosslinked Aß40 peptide.

    Exposure time: 48 seconds

    All lanes: Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (AB254345) at 1/1000 dilution

    Lane 1: BSA-crosslinked beta-Amyloid (Aβ42) 1-42 peptide 20 ng

    Lane 2: BSA-crosslinked beta-Amyloid (Aβ40) 1-40 peptide 20 ng

    Lane 3: BSA-crosslinked beta-Amyloid (Aβ39) 1-39 peptide 20 ng

    Lane 4: BSA-crosslinked beta-Amyloid (Aβ38) 1-38 peptide 20 ng

    Lane 5: BSA-crosslinked beta-Amyloid (Aβ37) 1-37 peptide 20 ng

    Lane 6: BSA-crosslinked beta-Amyloid (Aβ36) 1-36 peptide 20 ng

    Lane 7: BSA-crosslinked beta-Amyloid (Aβ16) 1-16 peptide 20 ng

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa

    Observed band size: 4 kDa, 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling beta-Amyloid 1-40 with ab254345 at 1/5000 dilutiob, followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Negative control: no staining on human cerebrum. The section was incubated with ab254345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345), expandable thumbnail

    Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345)

    Dot blot analysis of beta-Amyloid 1-40 using ab254345 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

    The samples are:

    Lane 1: Beta-Amyloid (Aβ42) 1-42 peptide

    Lane 2: Beta-Amyloid (Aβ40) 1-40 peptide

    Lane 3: Beta-Amyloid (Aβ39) 1-39 peptide

    Lane 4: Beta-Amyloid (Aβ38) 1-38 peptide

    Lane 5: Beta-Amyloid (Aβ37) 1-37 peptide

    Lane 6: Beta-Amyloid (Aβ36) 1-36 peptide

    Lane 7: Beta-Amyloid (Aβ16) 1-16 peptide

    Exposure time: 3 minutes

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345), expandable thumbnail

    Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345)

    Indirect ELISA using ab254345 at 0 - 1000 ng/mL.

    Antigen concentration is 1000 ng/mL.

    Secondary antibody is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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