AB289991

Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Amyloid-beta precursor protein antibody. Carrier free. Suitable for Dot, WB, I-ELISA, IHC-P and reacts with Human, Synthetic peptide - Human samples. Cited in 1 publication.

View Alternative Names

A4, AD1, APP, Amyloid-beta precursor protein, ABPP, APPI, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta (A4) precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, CVAP, PN-II

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

This data was developed using ab254345, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human Alzheimer's cerebrum tissue labelling beta-Amyloid 1-40 with ab254345 at 1/5000 dilution, followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Plaque staining on the human Alzheimer's cerebrum (PMID : 10912918). The section was incubated with ab254345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

This data was developed using ab254345, the same antibody clone in a different buffer formμlation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling beta-Amyloid 1-40 with ab254345 at 1/5000 (0.107 μg/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control : no staining on human cerebrum.The section was incubated with ab254345 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

I-ELISA

Supplier Data

Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

This data was developed using ab254345, the same antibody clone in a different buffer formulation.

Indirect ELISA using ab254345 at 0 - 1000 ng/mL.

Antigen concentration is 1000 ng/mL.

Secondary antibody is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Lab

Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

This data was developed using 254345, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The band over 75 KD is the BSA-crosslinked Aß40 peptide.

Exposure time : 48 seconds

All lanes:

Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (<a href='/en-us/products/primary-antibodies/beta-amyloid-1-40-antibody-epr23712-2-ab254345'>ab254345</a>) at 1/1000 dilution

Lane 1:

BSA-crosslinked beta-Amyloid (Aβ42) 1-42 peptide 20 ng

Lane 2:

BSA-crosslinked beta-Amyloid (Aβ40) 1-40 peptide 20 ng

Lane 3:

BSA-crosslinked beta-Amyloid (Aβ39) 1-39 peptide 20 ng

Lane 4:

BSA-crosslinked beta-Amyloid (Aβ38) 1-38 peptide 20 ng

Lane 5:

BSA-crosslinked beta-Amyloid (Aβ37) 1-37 peptide 20 ng

Lane 6:

BSA-crosslinked beta-Amyloid (Aβ36) 1-36 peptide 20 ng

Lane 7:

BSA-crosslinked beta-Amyloid (Aβ16) 1-16 peptide 20 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 86 kDa

Observed band size: 4 kDa,86 kDa

false

Lab

Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

This data was developed using ab254345, the same antibody clone in a different buffer formulation.

Dot blot analysis of beta-Amyloid 1-40 using ab254345 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

The samples are :

Lane 1 : Beta-Amyloid (Aβ42) 1-42 peptide

Lane 2 : Beta-Amyloid (Aβ40) 1-40 peptide

Lane 3 : Beta-Amyloid (Aβ39) 1-39 peptide

Lane 4 : Beta-Amyloid (Aβ38) 1-38 peptide

Lane 5 : Beta-Amyloid (Aβ37) 1-37 peptide

Lane 6 : Beta-Amyloid (Aβ36) 1-36 peptide

Lane 7 : Beta-Amyloid (Aβ16) 1-16 peptide

Exposure time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Unconjugated

Anti-beta Amyloid 1-40 antibody [EPR23712-2]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23712-2

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Dot, I-ELISA, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab289991 is the carrier-free version of ab254345

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Amyloid 1-40 also known as amyloid beta 40 is a peptide predominantly composed of 40 amino acids and it is a major product of amyloid precursor protein (APP) cleavage. Its molecular mass is approximately 4.3 kDa. This peptide is expressed in the brain tissue and cerebrospinal fluid. Beta amyloid engages in forming amyloid plaques which are deposits found most typically in the brain of patients with neurodegenerative conditions.

Biological function summary

Small soluble oligomers of beta amyloid interact with neuronal cells leading to synaptic dysfunction and cell death. Beta amyloid 1-40 molecules do not normally function in isolation; they often assemble into beta amyloid plaques a complex structure that can contribute to cellular anomalies. Amyloid beta production and its aggregation play parts in a process that disrupts neural communication impacting memory and learning processes.

Pathways

The amyloidogenic pathway involves beta amyloid where amyloid precursor protein (APP) is cleaved by beta-secretase and gamma-secretase to generate beta amyloid peptides including amyloid beta 40. This molecule is associated with the oxidation and inflammation pathways where it can interact with other proteins like presenilin. Presenilin is a part of the gamma-secretase complex that influences amyloid production thereby affecting the pathological aggregation process.

Beta amyloid 1-40 is closely linked to Alzheimer's disease a condition marked by cognitive decline and neural loss. The presence of amyloid plaques which are high in amyloid beta content is a characteristic marker of this disease. Furthermore beta amyloid is also associated with cerebral amyloid angiopathy a disorder where the peptide accumulates in the walls of the cerebral blood vessels. In this context tau protein another Alzheimer's-associated protein shows pathological changes influenced by the beta amyloid-induced plaque formation and contributes to neurofibrillary tangles formation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis (PubMed : 25122912). Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(o) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapses in axons (PubMed : 17062754, PubMed : 23011729). Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.. Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Amyloid-beta peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts.. Amyloid-beta protein 42. More effective reductant than amyloid-beta protein 40. May activate mononuclear phagocytes in the brain and elicit inflammatory responses.. Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain.. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.

See full target information APP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2410229 PubMed39804980

2025

Molecular Determinants of Protein Pathogenicity at the Single-Aggregate Level.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Urbanek,Emma F Garland,Emily E Prescott,Marianne C King,Anna Olerinyova,Hollie E Wareing,Nia Georgieva,Ellie L Bradshaw,Svetomir B Tzokov,Alexander Knight,Alexander I Tartakovskii,Tarja Malm,J Robin Highley,Suman De

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise