Rabbit Recombinant Monoclonal Amyloid-beta precursor protein antibody. Carrier free. Suitable for Dot, WB, I-ELISA, IHC-P and reacts with Human, Synthetic peptide - Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178801.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)")
![Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--western-blot-img172156.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174066.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)")
![Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--dot-blot-img171157.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)")
![Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--indirect-elisa-img167961.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (AB289991)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | I-ELISA | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested |
| Synthetic peptide - Human | Not recommended | Tested | Tested | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Version
Target data
Function
Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis (PubMed:25122912). Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapes in axons (PubMed:17062754, PubMed:23011729). Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Amyloid-beta protein 42 is a more effective reductant than amyloid-beta protein 40. Amyloid-beta peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. APP42-beta may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts.Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain.The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).
Alternative names
Amyloid-beta precursor protein, APP, ABPP, APPI, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, CVAP, PN-II, APP, A4, AD1
Recommended products
Rabbit Recombinant Monoclonal Amyloid-beta precursor protein antibody. Carrier free. Suitable for Dot, WB, I-ELISA, IHC-P and reacts with Human, Synthetic peptide - Human samples.
Alternative names
Amyloid-beta precursor protein, APP, ABPP, APPI, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, CVAP, PN-II, APP, A4, AD1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23712-2
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab289991 is the carrier-free version of Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Beta Amyloid 1-40 also known as amyloid beta 40 is a peptide predominantly composed of 40 amino acids and it is a major product of amyloid precursor protein (APP) cleavage. Its molecular mass is approximately 4.3 kDa. This peptide is expressed in the brain tissue and cerebrospinal fluid. Beta amyloid engages in forming amyloid plaques which are deposits found most typically in the brain of patients with neurodegenerative conditions.
- Biological function summary
Small soluble oligomers of beta amyloid interact with neuronal cells leading to synaptic dysfunction and cell death. Beta amyloid 1-40 molecules do not normally function in isolation; they often assemble into beta amyloid plaques a complex structure that can contribute to cellular anomalies. Amyloid beta production and its aggregation play parts in a process that disrupts neural communication impacting memory and learning processes.
- Pathways
The amyloidogenic pathway involves beta amyloid where amyloid precursor protein (APP) is cleaved by beta-secretase and gamma-secretase to generate beta amyloid peptides including amyloid beta 40. This molecule is associated with the oxidation and inflammation pathways where it can interact with other proteins like presenilin. Presenilin is a part of the gamma-secretase complex that influences amyloid production thereby affecting the pathological aggregation process.
- Associated diseases and disorders
Beta amyloid 1-40 is closely linked to Alzheimer's disease a condition marked by cognitive decline and neural loss. The presence of amyloid plaques which are high in amyloid beta content is a characteristic marker of this disease. Furthermore beta amyloid is also associated with cerebral amyloid angiopathy a disorder where the peptide accumulates in the walls of the cerebral blood vessels. In this context tau protein another Alzheimer's-associated protein shows pathological changes influenced by the beta amyloid-induced plaque formation and contributes to neurofibrillary tangles formation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178801.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
This data was developed using Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human Alzheimer's cerebrum tissue labelling beta-Amyloid 1-40 with Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345 at 1/5000 dilution, followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Plaque staining on the human Alzheimer's cerebrum (PMID: 10912918). The section was incubated with Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--western-blot-img172156.jpg "Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)")
Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
This data was developed using 254345, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The band over 75 KD is the BSA-crosslinked Aß40 peptide.
Exposure time: 48 seconds
All lanes: Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] (Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345) at 1/1000 dilution
Lane 1: BSA-crosslinked beta-Amyloid (Aβ42) 1-42 peptide 20 ng
Lane 2: BSA-crosslinked beta-Amyloid (Aβ40) 1-40 peptide 20 ng
Lane 3: BSA-crosslinked beta-Amyloid (Aβ39) 1-39 peptide 20 ng
Lane 4: BSA-crosslinked beta-Amyloid (Aβ38) 1-38 peptide 20 ng
Lane 5: BSA-crosslinked beta-Amyloid (Aβ37) 1-37 peptide 20 ng
Lane 6: BSA-crosslinked beta-Amyloid (Aβ36) 1-36 peptide 20 ng
Lane 7: BSA-crosslinked beta-Amyloid (Aβ16) 1-16 peptide 20 ng
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 4 kDa, 86 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174066.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
This data was developed using Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345, the same antibody clone in a different buffer formμlation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling beta-Amyloid 1-40 with Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345 at 1/5000 (0.107 μg/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control: no staining on human cerebrum.The section was incubated with Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--dot-blot-img171157.jpg "Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)")
Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
This data was developed using Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345, the same antibody clone in a different buffer formulation.
Dot blot analysis of beta-Amyloid 1-40 using Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.
The samples are:
Lane 1: Beta-Amyloid (Aβ42) 1-42 peptide
Lane 2: Beta-Amyloid (Aβ40) 1-40 peptide
Lane 3: Beta-Amyloid (Aβ39) 1-39 peptide
Lane 4: Beta-Amyloid (Aβ38) 1-38 peptide
Lane 5: Beta-Amyloid (Aβ37) 1-37 peptide
Lane 6: Beta-Amyloid (Aβ36) 1-36 peptide
Lane 7: Beta-Amyloid (Aβ16) 1-16 peptide
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST
![Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991), expandable thumbnail](https://content.abcam.com/products/images/beta-amyloid-1-40-antibody-epr23712-2-bsa-and-azide-free-ab289991--indirect-elisa-img167961.jpg "Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)")
Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
This data was developed using Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345, the same antibody clone in a different buffer formulation.
Indirect ELISA using Anti-beta Amyloid 1-40 antibody [EPR23712-2] ab254345 at 0 - 1000 ng/mL.
Antigen concentration is 1000 ng/mL.
Secondary antibody is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.
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