Anti-beta Amyloid antibody [MOAB-2] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [MOAB-2] - BSA and Azide free (AB230297)

IHC image of ab126649 staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126649, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab126649).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid antibody [MOAB-2] - BSA and Azide free (AB230297)

IHC image of ab126649 staining in transgenic mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126649, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab126649).

• WB

Collaborator

Western blot - Anti-beta Amyloid antibody [MOAB-2] - BSA and Azide free (AB230297)

This data was developed using ab126649, the same antibody clone in a different buffer formulation.

Lanes 1 - 2:

Western blot - Anti-beta Amyloid antibody [MOAB-2] (<a href='/en-us/products/primary-antibodies/beta-amyloid-antibody-moab-2-ab126649'>ab126649</a>) at 0.5 µg/mL

Lanes 3 - 4:

Anti-beta Amyloid antibody [6E10] at 0.5 µg/mL

Lanes 5 - 6:

Anti-beta Amyloid antibody [22C11] at 2 µg/mL

Lanes 1, 3 and 5:

Synthetic beta Amyloid 42 at 100 pmoles

Lanes 2, 4 and 6:

5xFAD transgenic mouse (12 months) lysate at 25 µg

Secondary

All lanes:

Anti-mouse IgG

Predicted band size: 87 kDa

true

This image is courtesy of M.J. LaDu, Univ of IL, Chicago