AB205301

Anti-beta Casein antibody [EPR12179]

Name: Anti-beta Casein antibody [EPR12179] (ab205301) | Abcam
Brand: Abcam Limited
SKU: AB205301
Price: 315 USD
Availability: InStock
Rating: 4 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(5 Publications)

Rabbit Recombinant Monoclonal beta Casein antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CASB, CSN2, Beta-casein

2 Images

Supplier Data

Immunoprecipitation - Anti-beta Casein antibody [EPR12179] (AB205301)

beta Casein was immunoprecipitated from 1mg of Human milk lysate with ab205301 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab205301 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

Lane 1 : Human milk lysate10 μg (Input). Lane 2 : ab205301 IP in Human milk lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205301 in Human milk lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-beta Casein antibody [EPR12179] (ab205301)

Predicted band size: 25 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-beta Casein antibody [EPR12179] (AB205301)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta Casein antibody [EPR12179] (ab205301) at 1/1000 dilution

Lane 1:

Human milk lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Exposure time: 5s

Carrier free

Anti-beta Casein antibody [EPR12179] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12179

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-casein is a major component of milk proteins known also simply as casein or beta-casein protein. This protein plays an important structural role in forming micelles which ensures efficient transport and digestion of calcium and phosphate in milk. Beta-casein has a molecular weight of about 24 kDa. It is primarily expressed in the mammary gland produced during lactation. Analyzing beta-casein often involves techniques like casein SDS PAGE which helps in confirming its molecular mass and purity.

Biological function summary

Beta-casein is involved in both nutritional and protective functions. In the mammary gland it is part of the casein micelle complex which includes other caseins like alpha-casein and kappa-casein. These micelles improve the stability and solubility of milk proteins. Beta-casein’s phosphorylation allows binding with calcium ions supporting skeletal development. This function is significant in neonatal growth as casein protein delivers amino acids critical for development.

Pathways

Beta-casein functions within pathways related to calcium transport and absorption. It interacts with proteins involved in the signaling pathways such as osteopontin enhancing mineral absorption. In milk production processes beta-casein supports micelle assembly and stabilization forming complexes that incorporate other milk proteins. These interactions are essential in ensuring the proper delivery of nutrients and minerals to developing offspring.

Beta-casein may play a role in dairy-related sensitivities and certain metabolic disorders. Some studies suggest its A1 variant could be linked to digestive discomfort in lactose-intolerant individuals. Beta-casein’s protein fragments such as beta-casomorphin are also under investigation for links to conditions like type 1 diabetes. In these contexts beta-casein's interaction with proteins involved in immune response might influence disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Important role in determination of the surface properties of the casein micelles.

See full target information CSN2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:412 PubMed41039499

2025

A comparative proteomic, transcriptomic and glycomic analysis of extracellular vesicle isolation techniques highlights ExoGAG efficiency for a more complete identification of breast milk molecular signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

María Pereira-Hernández,Rosaura Picáns-Leis,María E Vázquez-Mosquera,Nerea Lago-Baameiro,Pedro Fortes-González,Laura López-Valverde,Ana Barcia de la Iglesia,Laura Núñez-González,Susana B Bravo,María Pardo,María L Couce,Miguel A Garcia-Gonzalez

Nature communications 16:4865 PubMed40419484

2025

FOXA2 promotes metastatic competence in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Kawasaki,Sohrab Salehi,Yingqian A Zhan,Kevin Chen,Jun Ho Lee,Eralda Salataj,Hong Zhong,Parvathy Manoj,Dennis Kinyua,Barbara P Mello,Harsha Sridhar,Sam E Tischfield,Irina Linkov,Nicholas Ceglia,Matthew Zatzman,Eliyahu Havasov,Neil J Shah,Fanli Meng,Brian Loomis,Umesh K Bhanot,Esther Redin,Elisa de Stanchina,Pierre-Jacques Hamard,Richard P Koche,Andrew McPherson,Álvaro Quintanal-Villalonga,Sohrab P Shah,Joan Massagué,Charles M Rudin

Cellular and molecular gastroenterology and hepatology 19:101420 PubMed39414025

2024

The Human Milk-derived Peptide Drives Rapid Regulation of Macrophage Inflammation Responses in the Neonatal Intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Fuqiang Yuan,Xu Han,Masha Huang,Yinglin Su,Yiting Zhang,Mengyuan Hu,Xiang Yu,Weilai Jin,Yun Li,Le Zhang

Journal of extracellular vesicles 13:e12422 PubMed38602306

2024

Neonatal enteroids absorb extracellular vesicles from human milk-fed infant digestive fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Yung,Yang Zhang,Madeline Kuhn,Randall J Armstrong,Amy Olyaei,Molly Aloia,Brian Scottoline,Sarah F Andres

International journal of molecular sciences 21: PubMed32290104

2020

Identification and Characterization of the Lactating Mouse Mammary Gland Citrullinome.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyuan Li,Coleman H Young,Bryce Snow,Amanda O Christensen,M Kristen Demoruelle,Venkatesh V Nemmara,Paul R Thompson,Heather M Rothfuss,Brian D Cherrington

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Casein antibody [EPR12179]

Immunoprecipitation - Anti-beta Casein antibody [EPR12179] (AB205301)

Western blot - Anti-beta Casein antibody [EPR12179] (AB205301)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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Product promise