Anti-beta Casein antibody [EPR12179] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-beta Casein antibody [EPR12179] - BSA and Azide free (AB232662)

beta Casein was immunoprecipitated from 1mg of Human milk lysate with ab205301 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab205301 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

Lane 1 : Human milk lysate10 μg (Input). Lane 2 : ab205301 IP in Human milk lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205301 in Human milk lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205301).

All lanes:

Immunoprecipitation - Anti-beta Casein antibody [EPR12179] (<a href='/en-us/products/primary-antibodies/beta-casein-antibody-epr12179-ab205301'>ab205301</a>)

Predicted band size: 25 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-beta Casein antibody [EPR12179] - BSA and Azide free (AB232662)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205301).

All lanes:

Western blot - Anti-beta Casein antibody [EPR12179] - BSA and Azide free (ab232662) at 1/1000 dilution

Lane 1:

Human milk lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 25 kDa

false

Exposure time: 5s