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AB47972

Anti-beta Casein antibody [F20.14]

5

(1 Review)

|

(4 Publications)

Mouse Monoclonal beta Casein antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Human CSN2.

View Alternative Names

CASB, CSN2, Beta-casein

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

F20.14

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-Fr, WB, IHC-P

applications

Immunogen

Full Length Protein corresponding to Human CSN2. The exact immunogen used to generate this antibody is proprietary information.

P05814

Specificity

This antibody reacts with beta-casein. The reactivity with alpha-casein has not been tested.

This antibody does not react with alpha lactalbumin. This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.

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Alternative Products

Primary Antibodies

AB166596

Anti-Casein antibody

Western blot - Anti-Casein antibody (AB166596)

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  • 2
Primary Antibodies

AB188331

Anti-Casein antibody [EPR15762] - N-terminal

RabMAb|Recombinant

Immunoprecipitation - Anti-Casein antibody [EPR15762] - N-terminal (AB188331)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB188331

Anti-Casein antibody [EPR15762] - N-terminal

RabMAb|Recombinant

Immunoprecipitation - Anti-Casein antibody [EPR15762] - N-terminal (AB188331)

  • 0
  • 0
Primary Antibodies

AB178431

Anti-alpha Lactalbumin antibody [EPR12460]

RabMAb|Recombinant

Western blot - Anti-alpha Lactalbumin antibody [EPR12460] (AB178431)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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  • 88
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Fix with acetone.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-casein is a major component of milk proteins known also simply as casein or beta-casein protein. This protein plays an important structural role in forming micelles which ensures efficient transport and digestion of calcium and phosphate in milk. Beta-casein has a molecular weight of about 24 kDa. It is primarily expressed in the mammary gland produced during lactation. Analyzing beta-casein often involves techniques like casein SDS PAGE which helps in confirming its molecular mass and purity.
Biological function summary

Beta-casein is involved in both nutritional and protective functions. In the mammary gland it is part of the casein micelle complex which includes other caseins like alpha-casein and kappa-casein. These micelles improve the stability and solubility of milk proteins. Beta-casein’s phosphorylation allows binding with calcium ions supporting skeletal development. This function is significant in neonatal growth as casein protein delivers amino acids critical for development.

Pathways

Beta-casein functions within pathways related to calcium transport and absorption. It interacts with proteins involved in the signaling pathways such as osteopontin enhancing mineral absorption. In milk production processes beta-casein supports micelle assembly and stabilization forming complexes that incorporate other milk proteins. These interactions are essential in ensuring the proper delivery of nutrients and minerals to developing offspring.

Beta-casein may play a role in dairy-related sensitivities and certain metabolic disorders. Some studies suggest its A1 variant could be linked to digestive discomfort in lactose-intolerant individuals. Beta-casein’s protein fragments such as beta-casomorphin are also under investigation for links to conditions like type 1 diabetes. In these contexts beta-casein's interaction with proteins involved in immune response might influence disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important role in determination of the surface properties of the casein micelles.
See full target information CSN2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of mammary gland biology and neoplasia 24:245-256 PubMed31529195

2019

The Milk Protein Alpha-Casein Suppresses Triple Negative Breast Cancer Stem Cell Activity Via STAT and HIF-1alpha Signalling Pathways in Breast Cancer Cells and Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten E L Garner,Nathan J Hull,Andrew H Sims,Rebecca Lamb,Robert B Clarke

Breast cancer research and treatment 140:285-97 PubMed23881524

2013

Dynamic changes in high and low mammographic density human breast tissues maintained in murine tissue engineering chambers during various murine peripartum states and over time.

Applications

Unspecified application

Species

Unspecified reactive species

G L Chew,D Huang,C W Huo,T Blick,P Hill,J Cawson,H Frazer,M D Southey,J L Hopper,M A Henderson,I Haviv,E W Thompson

Cell cycle (Georgetown, Tex.) 11:3972-82 PubMed23047602

2012

The milk protein α-casein functions as a tumor suppressor via activation of STAT1 signaling, effectively preventing breast cancer tumor growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Gloria Bonuccelli,Remedios Castello-Cros,Franco Capozza,Ubaldo E Martinez-Outschoorn,Zhao Lin,Aristotelis Tsirigos,Jiao Xuanmao,Diana Whitaker-Menezes,Anthony Howell,Michael P Lisanti,Federica Sotgia

Hybridoma 4:341-50 PubMed3905581

1985

Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin.

Applications

Unspecified application

Species

Unspecified reactive species

J Burchell,J Bartek,J Taylor-Papadimitriou
View all publications
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Product promise

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