AB19381

Anti-beta Catenin antibody [9F2]

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(10 Publications)

Mouse Monoclonal beta Catenin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CTNNB1.

View Alternative Names

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [9F2] (AB19381)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [9F2] (AB19381)

Immunofluorescent analysis of Beta-Catenin using Beta-Catenin Monoclonal antibody (9F2) ab19381 shows staining in HeLa cells. Beta-Catenin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Beta-Catenin ab19381 at a dilution of 1 : 20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [9F2] (AB19381)

Immunofluorescent analysis of Beta-Catenin using Beta-Catenin Monoclonal antibody (9F2) ab19381 shows staining in U251 glioma cells. Beta-Catenin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Beta-Catenin ab19381 at a dilution of 1 : 20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

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Western blot - Anti-beta Catenin antibody [9F2] (AB19381)

All lanes:

Western blot - Anti-beta Catenin antibody [9F2] (ab19381) at 3 µg/mL

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

U-97 MG whole cell lysate at 30 µg

Lane 3:

COLO 205 whole cell lysate at 30 µg

Lane 4:

HCT 116 whole cell lysate at 30 µg

Lane 5:

HT-29 whole cell lysate at 30 µg

Lane 6:

MCF7 whole cell lysate at 30 µg

Lane 7:

MDA-MB-231 whole cell lysate at 30 µg

Lane 8:

A549 whole cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 85 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9F2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CTNNB1. The exact immunogen used to generate this antibody is proprietary information.

P35222

Specificity

This antibody detects beta catenin from Human samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Immunogen purified on amylose column.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.

Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Key downstream component of the canonical Wnt signaling pathway (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin : catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed : 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed : 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed : 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed : 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9 : SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).

See full target information CTNNB1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 32:213-226 PubMed38188679

2024

Silencing of peroxiredoxin 2 suppresses proliferation and Wnt/β-catenin pathway, and induces senescence in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xuegang Yang,Xianhong Xiang,Guohui Xu,Shi Zhou,Tianzhi An,Zhi Huang

Scientific reports 13:16920 PubMed37805544

2023

Muscarinic receptor agonist-induced βPix binding to β-catenin promotes colon neoplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Kunrong Cheng,Ahmed Chahdi,Shannon M Larabee,Mazen Tolaymat,Margaret H Sundel,Cinthia B Drachenberg,Min Zhan,Shien Hu,Anan H Said,Aaron C Shang,Guofeng Xie,Madeline Alizadeh,Natalia Sampaio Moura,Andrea C Bafford,Richelle T Williams,Nader N Hanna,Jean-Pierre Raufman

Life science alliance 6: PubMed37748809

2023

KCNQ potassium channels modulate Wnt activity in gastro-oesophageal adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

David Shorthouse,Lizhe Zhuang,Eric P Rahrmann,Cassandra Kosmidou,Katherine Wickham Rahrmann,Michael Hall,Benedict Greenwood,Ginny Devonshire,Richard J Gilbertson,Rebecca C Fitzgerald,Benjamin A Hall

Scientific reports 12:17318 PubMed36243826

2022

Lateral confined growth of cells activates Lef1 dependent pathways to regulate cell-state transitions.

Applications

Unspecified application

Species

Unspecified reactive species

Luezhen Yuan,Bibhas Roy,Prasuna Ratna,Caroline Uhler,G V Shivashankar

British journal of biomedical science 79:10067 PubMed35996510

2022

Micro RNA-640 Targeting SLIT1 Enhances Glioma Radiosensitivity by Restraining the Activation of Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yamei Zheng,Mingyue Xiao,Jingqiong Zhang,Fei Chang

Journal of medicinal chemistry 64:10934-10950 PubMed34309393

2021

Macrocyclic Immunoproteasome Inhibitors as a Potential Therapy for Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Min Jae Lee,Deepak Bhattarai,Hyeryung Jang,Ahreum Baek,In Jun Yeo,Seongsoo Lee,Zachary Miller,Sukyeong Lee,Jin Tae Hong,Dong-Eun Kim,Wooin Lee,Kyung Bo Kim

Cellular and molecular life sciences : CMLS 78:5647-5663 PubMed34128077

2021

Inhibitory control in neuronal networks relies on the extracellular matrix integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Egor Dzyubenko,Michael Fleischer,Daniel Manrique-Castano,Mina Borbor,Christoph Kleinschnitz,Andreas Faissner,Dirk M Hermann

Frontiers in molecular neuroscience 13:596039 PubMed33551744

2021

Ambroxol Upregulates Glucocerebrosidase Expression to Promote Neural Stem Cells Differentiation Into Neurons Through Wnt/β-Catenin Pathway After Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Hongfei Ge,Chao Zhang,Yang Yang,Weixiang Chen,Jun Zhong,Xuanyu Fang,Xuheng Jiang,Liang Tan,Yongjie Zou,Rong Hu,Yujie Chen,Hua Feng

Journal of neurochemistry 154:84-98 PubMed32141089

2020

Brain region-specific amyloid plaque-associated myelin lipid loss, APOE deposition and disruption of the myelin sheath in familial Alzheimer's disease mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ibrahim Kaya,Eva Jennische,Stefan Lange,Ahmet Tarik Baykal,Per Malmberg,John S Fletcher

Scientific reports 9:18393 PubMed31804556

2019

A dual inhibitor of the proteasome catalytic subunits LMP2 and Y attenuates disease progression in mouse models of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

In Jun Yeo,Min Jae Lee,Ahruem Baek,Zachary Miller,Deepak Bhattarai,Yu Mi Baek,Hyun Jung Jeong,Yun Kyung Kim,Dong-Eun Kim,Jin Tae Hong,Kyung Bo Kim

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