Anti-beta Catenin antibody

12 product images

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  Western blot - Anti-beta Catenin antibody (ab16051)

  Lane 1: Wild type HAP1 whole cell lysate (20 μg)
  Lane 2: CTNNB1 (β-catenin) knockout HAP1 whole cell lysate (20 μg)
  Lane 3: A431 whole cell lysate (20 μg)
  
  Lanes 1 - 3: Merged signal (red and green). Green - ab16051 observed at 95 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
  

  ab16051 was shown to specifically react with CTNNB1 (β-catenin) along with additional cross-reactive bands in wild-type HAP1 cells. No band was observed in knockout samples. Wild-type and CTNNB1 (β-catenin) knockout samples were subjected to SDS-PAGE. ab16051 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 0.25 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  All lanes: Western blot - Anti-beta Catenin antibody (ab16051)

  Predicted band size: 85 kDa

  Observed band size: 95 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)

  ab16051 staining CTNNB1 (β-catenin) in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab16051 at 1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (staining Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)

  IHC image of beta catenin staining in a formalin fixed, paraffin embedded normal human liver tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16051, 0.5 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)

  IHC image of beta catenin staining in a formalin fixed, paraffin embedded human colon adenocarcinamo tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16051, 0.5 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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  This image is courtesy of a customer review by Carl Hobbs.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)

  ab16051 staining beta Catenin in rat brain tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)

  ab16051 stained in MCF7cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16051 at 5 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (pseudo-colored red) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1hour at room temperature

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  Western blot - Anti-beta Catenin antibody (ab16051)

  A second band of 75 kDa was also detected in Hela whole cell lysates and A431 lysates. This smaller band was of equal intensity to the 94 kDa band in the A431 lysates (data not shown).

  All lanes: Western blot - Anti-beta Catenin antibody (ab16051) at 0.25 µg/mL

  Lane 1: HeLa whole cell lysate at 20 µg

  Lane 2: HeLa whole cell lysate at 20 µg with Human beta Catenin peptide (ab16377)

  Secondary

  All lanes: Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 85 kDa

  Observed band size: 75 kDa, 94 kDa

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  Western blot - Anti-beta Catenin antibody (ab16051)

  All lanes: Western blot - Anti-beta Catenin antibody (ab16051) at 0.25 µg/mL

  All lanes: Western blot - Recombinant Human beta Catenin protein (Tagged) (Recombinant Human beta Catenin protein (Tagged) ab63175) at 0.01 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 85 kDa

  Exposure time: 10s

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  Sandwich ELISA - Anti-beta Catenin antibody (ab16051)

  Standard Curve for Beta-Catenin (Analyte: beta Catenin protein (Tagged) (Recombinant Human beta Catenin protein (Tagged) ab63175)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [BDI080] to beta Catenin (ab19448) at 1ug/ml and Detector Antibody Rabbit polyclonal to beta Catenin (ab16051) at 0.1ug/ml.

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  This image is courtesy of a customer review by Carl Hobbs.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)

  ab16051 staining beta Catenin in developing gut tissue sections from mouse by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 2 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

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  This image is courtesy of a customer review submitted by Miss Helen Gillingham

  Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)

  ab16051 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to incubation with the primary antibody (at 10μg/ml) for 1 hour at 27°C. A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.

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  Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)

  ab16051 staining β-catenin in SW480 cells treated with XAV939 (XAV939, Tankyrase inhibitor ab120897), by ICC/IF. Increase of ß-catenin cytoplasmic expression and decrease in nuclear expression correlates with increased concentration of XAV939, as described in literature.

  The cells were incubated at 37°C for 6 hours in media containing different concentrations of XAV939, Tankyrase inhibitor ab120897 (XAV939) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16051 (1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.