Rabbit Polyclonal beta Catenin antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 61 publications.
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- Materials today. Bio 23:1008442023An model system based on calcium- and phosphate ion-induced hMSC spheroid mineralization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSteven Vermeulen,Kèvin Knoops,Hans Duimel,Maryam Parvizifard,Denis van Beurden,Carmen López-Iglesias,Stefan Giselbrecht,Roman Truckenmüller,Pamela Habibović,Zeinab Tahmasebi BirganiPubMed 38033367
- Cell biology international 47:63-742022Knockdown of long noncoding RNA MIAT attenuates hypoxia-induced cardiomyocyte injury by regulating the miR-488-3p/Wnt/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Zhang,Zhiling Zhang,Jie Hu,Shulan Zhou,Wenwei AiPubMed 36273414
- PLoS biology 20:e30018072022Cell shape anisotropy contributes to self-organized feather pattern fidelity in birds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCamille Curantz,Richard Bailleul,María Castro-Scherianz,Magdalena Hidalgo,Melina Durande,François Graner,Marie ManceauPubMed 36215298
- Pharmaceutics 14:2022Mimicking the Intestinal Host-Pathogen Interactions in a 3D In Vitro Model: The Role of the Mucus Layer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría García-Díaz,Maria Del Mar Cendra,Raquel Alonso-Roman,María Urdániz,Eduard Torrents,Elena MartínezPubMed 35893808
- Scientific reports 12:119182022Efficacy of auranofin as an inhibitor of desmoid progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKan Ito,Yoshihiro Nishida,Shunsuke Hamada,Koki Shimizu,Tomohisa Sakai,Bisei Ohkawara,Benjamin A Alman,Atsushi Enomoto,Kunihiro Ikuta,Hiroshi Koike,Jiarui Zhang,Kinji Ohno,Shiro ImagamaPubMed 35831372
- Kidney360 3:1228-12412022Stromal Transcription Factor 21 Regulates Development of the Renal Stroma Interaction with Wnt/-Catenin Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGal Finer,Yoshiro Maezawa,Shintaro Ide,Tuncer Onay,Tomokazu Souma,Rizaldy Scott,Xiaoyan Liang,Xiangmin Zhao,Gaurav Gadhvi,Deborah R Winter,Susan E Quaggin,Tomoko HayashidaPubMed 35919523
- Cancer cell international 21:6512021CircTUBGCP3 facilitates the tumorigenesis of lung adenocarcinoma by sponging miR-885-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Yang,Xin Fan,Yunfei Nie,Donglei Liu,Dengyan Zhu,Kai Wu,Yuan Zhang,Wenhua Li,Xiangyu Tian,Huaqi Wang,Yuxia FanPubMed 34872582
- EMBO reports 22:e532012021miR-424/503 modulates Wnt/β-catenin signaling in the mammary epithelium by targeting LRP6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErin A Nekritz,Ruth Rodriguez-Barrueco,Koon-Kiu Yan,Meredith L Davis,Rachel L Werner,Laura Devis-Jauregui,Partha Mukhopadhyay,Jiyang Yu,David Llobet-Navas,Jose SilvaPubMed 34633138
- Nature 596:291-2952021Regulated control of gene therapies by drug-induced splicing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlex Mas Monteys,Amiel A Hundley,Paul T Ranum,Luis Tecedor,Amy Muehlmatt,Euyn Lim,Dmitriy Lukashev,Rajeev Sivasankaran,Beverly L DavidsonPubMed 34321659
- Aging 13:13859-138752021Curcumin inhibits the proliferation and migration of vascular smooth muscle cells by targeting the chemerin / CMKLR1 / LCN2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaqiong He,Rongning Wang,Peng Zhang,Jianlong Yan,Nan Gong,Yuhang Li,Shaohong DongPubMed 34029211
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes Incubate with primary antibody for 10 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Xenopus laevis | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Xenopus laevis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Xenopus laevis | Dilution info - | Notes - |
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Target data
Function
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).
Alternative names
CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin
Recommended products
Rabbit Polyclonal beta Catenin antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 61 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.
- Biological function summary
This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.
- Pathways
Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.
- Associated diseases and disorders
Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-beta Catenin antibody (ab2365)
Lane 1: Western blot - Anti-beta Catenin antibody (ab2365) at 1/25 dilution
Lane 1: Western blot - Anti-beta Catenin antibody, prediluted (Anti-beta Catenin antibody, prediluted ab15180) at 1/25 dilution
All lanes: A431 cell lysate
Predicted band size: 85 kDa
Observed band size: 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab2365)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for beta Catenin using ab2365 at 1/250 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-beta Catenin antibody (ab2365)
Beta Catenin was immunoprecipitated using 0.5mg A431 whole cell extract, 5μg of Rabbit polyclonal to beta Catenin and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2365.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 85kDa; beta Catenin.All lanes: Immunoprecipitation - Anti-beta Catenin antibody (ab2365)
Predicted band size: 85 kDa
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