Rabbit Polyclonal beta Catenin antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Cow, Rat samples. Cited in 273 publications. Immunogen corresponding to Synthetic Peptide within Human CTNNB1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Images
Publications
- Stem cell research & therapy 14:3732023Mesenchymal stem cells lose the senescent phenotype under 3D cultivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesO Krasnova,A Kovaleva,A Saveleva,K Kulakova,O Bystrova,M Martynova,A Domnina,J Sopova,I NeganovaPubMed 38111010
- BMC molecular and cell biology 24:122023The DNA demethylation-regulated SFRP2 dictates the progression of endometriosis via activation of the Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei Yang,Lin Li,Xiaojie Huang,Hui Xing,Li Hong,Chunfan JiangPubMed 36991319
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.097% Sodium azide
Constituents: Whole serum- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human CTNNB1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P35222
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Rat | Expected | Expected | Tested |
| Cow | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/4000 | Notes - |
Species Cow | Dilution info 1/4000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
Species Cow | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).
Alternative names
Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286, CTNNB1
Recommended products
Rabbit Polyclonal beta Catenin antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Cow, Rat samples. Cited in 273 publications. Immunogen corresponding to Synthetic Peptide within Human CTNNB1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Alternative names
Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286, CTNNB1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.097% Sodium azide
Constituents: Whole serum- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human CTNNB1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P35222
- Purity
Whole antiserum
- Specificity
Reacts in dot blot with beta-catenin peptide 768-781 conjugated to BSA.
In immunoblots, reacts with a 94kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cells. Specific staining is inhibited following pre-incubation of the antiserum with the beta-catenin peptide.
Shows no reactivity with BSA conjugated alpha catenin peptide (amino acids 890-901).
The antibody does not cross react with α-catenin or γ-catenin (plakoglobin).- Concentration
- Purification notes
Delipidized antiserum.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Western blot - Anti-beta Catenin antibody (ab6302)
This image is courtesy of an Abreview submitted by Mike Campa on 4 April 2006.
All lanes: Western blot - Anti-beta Catenin antibody (ab6302) at 1/4000 dilution
Lane 1: 5ug human lung tumour lysate.
Lane 2: 10ug human lung tumour lysate.
Lane 3: 20ug human lung tumour lysate.
SecondaryAll lanes: Goat anti-Rabbit IgG (H&L)HRP
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 95 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling beta Catenin with ab6302 at 1/20,000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab6302)
Immunocytochemistry analysis of bovine kidney cells labeling beta Catenin with ab6302 at 1/20,000 dilution. Cells were fixed and permeabilized with Methanol followed by Acetone. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Cells were counterstained with DAPI (blue) to stain nuclei.
Western blot - Anti-beta Catenin antibody (ab6302)
All lanes: Western blot - Anti-beta Catenin antibody (ab6302) at 1/8000 dilution
All lanes: Bovine kidney tissue lysate
Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling beta Catenin with ab6302 at 1/20,000 dilution.
This image is courtesy of a customer review submitted by Jennifer SchnabelImmunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab6302)
ab6302 at 1/2000 dilution staining beta Catenin in human HeLa cells by immunocytochemistry/ immunofluorescence. Sections were paraformaldehyde fixed, permeabilized in 0.3% Triton X prior to blocking in 5% BSA for 2 hours at 27°C and then incubated with ab6302 for 8 hour at 4°C. Alexa fluor® 488 goat polyclonal, diluted 1/1000, was used as the secondary antibody. Counterstaining with DAPI.
This image is courtesy of a customer review submitted by Roderick BensonImmunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab6302)
ab6302 at 1/500 dilution staining beta Catenin in human breast cells by immunocytochemistry/ immunofluorescence. Sections were formaldehyde fixed, permeabilized in 0.5% digitonin prior to incubating with ab6302 for 1 hour. Alexa fluor® 488 goat polyclonal, diluted 1/500, was used as the secondary antibody. Treated samples recieved 10um GSK-3 Inhibitor X.
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab6302)
This picture was kindly supplied as part of the review submitted by Mohaiza Dashwood. Immunofluorescence of H29 cells stained with DAPI (blue) and rabbit polyclonal anti-beta-catenin (ab6302), 1/2000) with Alexa Fluor 488 (green) from Molecular Probes.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com