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AB265591

Anti-beta Catenin antibody [BLR086G]

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(7 Publications)

Rabbit Recombinant Monoclonal beta Catenin antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CTNNB1 aa 700 to C-terminus.

View Alternative Names

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

9 Images
Flow Cytometry - Anti-beta Catenin antibody [BLR086G] (AB265591)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-beta Catenin antibody [BLR086G] (AB265591)

Flow cytometry overlay histogram of 4% formaldehyde fixed FaDu cells permeabilized with 90% methanol labeling human beta Catenin with ab265591 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] (AB265591)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] (AB265591)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma, labeling beta Catenin with ab265591. HRP-conjugated goat anti-rabbit IgG used as the secondary antibody. DAPI counterstain.

Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] (AB265591)
  • ICC

Supplier Data

Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] (AB265591)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 (Human ovarian carcinoma cell line) cells labeling beta Catenin with ab265591 at 1/100 dilution. Detection : DAB.

Flow Cytometry - Anti-beta Catenin antibody [BLR086G] (AB265591)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-beta Catenin antibody [BLR086G] (AB265591)

Flow cytometry overlay histogram of 4% formaldehyde fixed FaDu cells permeabilized with 90% methanol labeling mouse beta Catenin with ab265591 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] (AB265591)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] (AB265591)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling beta Catenin with ab265591 at 1/100 dilution. Detection : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] (AB265591)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] (AB265591)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human plasmacytoma tissue labeling beta Catenin with ab265591 at 1/100 dilution. Detection : DAB.

Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (AB265591)
  • IP

Supplier Data

Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (AB265591)

beta Catenin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265591 at 20 μl per reaction. Western blot was performed from the immunoprecipitate using ab265591 at 1/1000 dilution.

Lane 1 : ab265591 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 3 secs.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (ab265591)

Predicted band size: 85 kDa

false

Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (AB265591)
  • IP

Supplier Data

Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (AB265591)

beta Catenin was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab265591 at 20 μl per reaction. Western blot was performed from the immunoprecipitate using ab265591 at 1/1000 dilution.

Lane 1 : ab265591 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 3 secs.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (ab265591)

Predicted band size: 85 kDa

false

Western blot - Anti-beta Catenin antibody [BLR086G] (AB265591)
  • WB

Supplier Data

Western blot - Anti-beta Catenin antibody [BLR086G] (AB265591)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-beta Catenin antibody [BLR086G] (ab265591) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 50 µg

Lane 2:

LNCaP whole cell lysate at 50 µg

Lane 3:

K562 whole cell lysate at 50 µg

Lane 4:

GaMG whole cell lysate at 50 µg

Lane 5:

Jurkat whole cell lysate at 50 µg

Lane 6:

A549 whole cell lysate at 50 µg

Lane 7:

MCF7 whole cell lysate at 50 µg

Lane 8:

HeLa whole cell lysate at 50 µg

Lane 9:

TCMK whole cell lysate at 50 µg

Lane 10:

NIH/3T3 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 85 kDa

false

Exposure time: 10s

  • Unconjugated

    Anti-beta Catenin antibody [BLR086G] - BSA free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR086G

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, IP, ICC, Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human CTNNB1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P35222

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p>Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS.
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.
Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key downstream component of the canonical Wnt signaling pathway (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). Also acts as a coactivator for other transcription factors, such as NR5A2 (PubMed : 22187462). Promotes epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via driving transcription of CTNNB1/TCF-target genes (PubMed : 29910125). Involved in the regulation of cell adhesion, as component of an E-cadherin : catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed : 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed : 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed : 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed : 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9 : SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).
See full target information CTNNB1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:325 PubMed41044576

2025

Kaempferol triggers cellular senescence via CDK1 ubiquitination in HCC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Damin Liang,Min Tian,Guomei Hu,Yu Zhang,Lunyou Zhang,Juqi Chen,Xin Shen,Huayong Jian,Peng Tian,Tingchao Li,Xiaoju Cheng

Cell biology and toxicology 40:108 PubMed39630363

2024

The mechanism of sevoflurane affecting ovarian cancer cell proliferation and migration by regulating RNA methylase TRDMT1 to activate the β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Huang,Xuewei Lao,Chengyan He,Jia Wang,Ying Pan

RSC advances 14:1527-1537 PubMed38179095

2024

Enhancing osteogenic differentiation of MC3T3-E1 cells during inflammation using UPPE/β-TCP/TTC composites the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Lin Li,Ya-Xin Wu,Yu-Xiao Zhang,Jing Mao,Zhi-Xing Zhang

Bioactive materials 20:598-609 PubMed35846837

2022

Carboxymethyl chitosan-alginate enhances bone repair effects of magnesium phosphate bone cement by activating the FAK-Wnt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Yu,Tian Gao,Wei Li,Jian Yang,Yinchu Liu,Yanan Zhao,Ping He,Xuefeng Li,Weichun Guo,Zhengfu Fan,Honglian Dai

Evidence-based complementary and alternative medicine : eCAM 2022:9302403 PubMed35502178

2022

Salvianolic Acid B Suppresses Non-Small-Cell Lung Cancer Metastasis through PKM2-Independent Metabolic Reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhang,Jianming Tang,Yu Cao,Tianhu Wang

Cancer medicine 11:1589-1604 PubMed35166053

2022

Long noncoding RNASEH1-AS1 exacerbates the progression of non-small cell lung cancer by acting as a ceRNA to regulate microRNA-516a-5p/FOXK1 and thereby activating the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Zhang,Jian Huang,Ke Lou,Hui Ouyang

Experimental and therapeutic medicine 22:1228 PubMed34539824

2021

LINC01094 promotes the invasion of ovarian cancer cells and regulates the Wnt/β-catenin signaling pathway by targeting miR-532-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Chen,Yanlin Liu,Ping Liu,Qiuxiang Dai,Peiliang Wang
View all publications
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