Anti-beta Catenin antibody [BLR086G] (ab265591)

Rabbit Recombinant Monoclonal beta Catenin antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CTNNB1 aa 700 to C-terminus.

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Images

Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] (AB265591), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human CTNNB1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35222

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC	IP	WB	Flow Cyt
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100.00000 - 1/500.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100.00000 - 1/500.00000	Notes Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes 20 μl/mg lysate.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1 µL for 10⁶ Cells	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information CTNNB1

Function

Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).

Alternative names

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human CTNNB1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35222
Clone number: BLR086G
Purification technique: Affinity purification
Concentration
Purification notes: Purified from TCS.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.

Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Associated diseases and disorders

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.

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9 product images

Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] (ab265591)
Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 (Human ovarian carcinoma cell line) cells labeling beta Catenin with ab265591 at 1/100 dilution. Detection: DAB.
Western blot - Anti-beta Catenin antibody [BLR086G] (ab265591)
Cells prepared using NETN lysis buffer.
All lanes: Western blot - Anti-beta Catenin antibody [BLR086G] (ab265591) at 1/1000 dilution
Lane 1: HepG2 whole cell lysate at 50 µg
Lane 2: LNCaP whole cell lysate at 50 µg
Lane 3: K562 whole cell lysate at 50 µg
Lane 4: GaMG whole cell lysate at 50 µg
Lane 5: Jurkat whole cell lysate at 50 µg
Lane 6: A549 whole cell lysate at 50 µg
Lane 7: MCF7 whole cell lysate at 50 µg
Lane 8: HeLa whole cell lysate at 50 µg
Lane 9: TCMK whole cell lysate at 50 µg
Lane 10: NIH/3T3 whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 85 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] (ab265591)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling beta Catenin with ab265591 at 1/100 dilution. Detection: DAB.
Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (ab265591)
beta Catenin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265591 at 20 μl per reaction. Western blot was performed from the immunoprecipitate using ab265591 at 1/1000 dilution.

Lane 1: ab265591 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
Cells prepared using NETN lysis buffer.
All lanes: Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (ab265591)
Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] (ab265591)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human plasmacytoma tissue labeling beta Catenin with ab265591 at 1/100 dilution. Detection: DAB.
Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (ab265591)
beta Catenin was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab265591 at 20 μl per reaction. Western blot was performed from the immunoprecipitate using ab265591 at 1/1000 dilution.

Lane 1: ab265591 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
Cells prepared using NETN lysis buffer.
All lanes: Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (ab265591)
Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] (ab265591)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma, labeling beta Catenin with ab265591. HRP-conjugated goat anti-rabbit IgG used as the secondary antibody. DAPI counterstain.
Flow Cytometry - Anti-beta Catenin antibody [BLR086G] (ab265591)
Flow cytometry overlay histogram of 4% formaldehyde fixed FaDu cells permeabilized with 90% methanol labeling mouse beta Catenin with ab265591 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 650) (Goat Anti-Rabbit IgG H&L (DyLight® 650) ab96886).
Flow Cytometry - Anti-beta Catenin antibody [BLR086G] (ab265591)
Flow cytometry overlay histogram of 4% formaldehyde fixed FaDu cells permeabilized with 90% methanol labeling human beta Catenin with ab265591 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 650) (Goat Anti-Rabbit IgG H&L (DyLight® 650) ab96886).