Rabbit Recombinant Monoclonal beta Catenin antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CTNNB1 aa 700 to C-terminus.
Images
![Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunocytochemistry-img69653.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069)")
![Western blot - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--western-blot-img186028.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19475.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069)")
![Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunoprecipitation-img69459.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83808.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (AB272069)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human CTNNB1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35222
Reactivity data
Select an application| IHC-P | ICC | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).
Alternative names
CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin
Recommended products
Rabbit Recombinant Monoclonal beta Catenin antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CTNNB1 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human CTNNB1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35222
- Clone number
- BLR086G
- Purification technique
- Affinity purification
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab272069 is the BSA-free version of Anti-beta Catenin antibody [BLR086G] ab265591.
This product is sold under License from Bethyl Laboratories, Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.
- Biological function summary
This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.
- Pathways
Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.
- Associated diseases and disorders
Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunocytochemistry-img69653.jpg "Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)")
Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)
Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 (Human ovarian carcinoma cell line) cells labeling beta Catenin with Anti-beta Catenin antibody [BLR086G] ab265591 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-beta Catenin antibody [BLR086G] ab265591).
![Western blot - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--western-blot-img186028.jpg "Western blot - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)")
Western blot - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)
Detection: Chemiluminescence.Lysates prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-beta Catenin antibody [BLR086G] ab265591).
All lanes: Western blot - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069) at 1/1000 dilution
Lane 1: Hep-G2 whole cell lysate at 50 µg
Lane 2: LNCaP whole cell lysate at 50 µg
Lane 3: K-562 whole cell lysate at 50 µg
Lane 4: GaMG whole cell lysate at 50 µg
Lane 5: Jurkat whole cell lysate at 50 µg
Lane 6: A-549 whole cell lysate at 50 µg
Lane 7: MCF-7 whole cell lysate at 50 µg
Lane 8: HeLa whole cell lysate at 50 µg
Lane 9: TCMK-1 whole cell lysate at 50 µg
Lane 10: NIH 3T3 whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 85 kDa
Exposure time: 10s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19475.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling beta Catenin with Anti-beta Catenin antibody [BLR086G] ab265591 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-beta Catenin antibody [BLR086G] ab265591).
![Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunoprecipitation-img69459.jpg "Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)")
Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)
beta Catenin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-beta Catenin antibody [BLR086G] ab265591 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using Anti-beta Catenin antibody [BLR086G] ab265591 at 1/1000 dilution.
Lane 1: Anti-beta Catenin antibody [BLR086G] ab265591 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
Cells prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-beta Catenin antibody [BLR086G] ab265591).
All lanes: Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (Anti-beta Catenin antibody [BLR086G] ab265591)
Predicted band size: 85 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83808.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human plasmacytoma tissue labeling beta Catenin with Anti-beta Catenin antibody [BLR086G] ab265591 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-beta Catenin antibody [BLR086G] ab265591).
![Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunoprecipitation-img69621.jpg "Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)")
Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)
beta Catenin was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with Anti-beta Catenin antibody [BLR086G] ab265591 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using Anti-beta Catenin antibody [BLR086G] ab265591 at 1/1000 dilution.
Lane 1: Anti-beta Catenin antibody [BLR086G] ab265591 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
Cells prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-beta Catenin antibody [BLR086G] ab265591).
All lanes: Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] (Anti-beta Catenin antibody [BLR086G] ab265591)
Predicted band size: 85 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img182669.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma, labeling beta Catenin with Anti-beta Catenin antibody [BLR086G] ab265591. HRP-conjugated goat anti-rabbit IgG used as the secondary antibody. DAPI counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-beta Catenin antibody [BLR086G] ab265591).
![Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunoprecipitation-img69621.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-blr086g-bsa-free-ab272069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img182669.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)")
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