Mouse Recombinant Monoclonal beta Catenin antibody. Carrier free. Suitable for Protein Array, IHC-P, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CTNNB1.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (AB237825), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-rctnnb1-2173-bsa-and-azide-free-ab237825--immunocytochemistry-immunofluorescence-img95214.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (AB237825)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (AB237825), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-rctnnb1-2173-bsa-and-azide-free-ab237825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129302.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (AB237825)")
![Protein Array - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (AB237825), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-rctnnb1-2173-bsa-and-azide-free-ab237825--protein-array-img140158.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (AB237825)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CTNNB1. Database link P35222
Reactivity data
Select an application| Protein Array | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 mins at RT. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).
Alternative names
CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin
Recommended products
Mouse Recombinant Monoclonal beta Catenin antibody. Carrier free. Suitable for Protein Array, IHC-P, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CTNNB1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CTNNB1. Database link P35222
- Carrier free
- Yes
- Clone number
- rCTNNB1/2173
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab237825 is the carrier-free version of Anti-beta Catenin antibody [rCTNNB1/2173] ab237983.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.
- Biological function summary
This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.
- Pathways
Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.
- Associated diseases and disorders
Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (ab237825), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-rctnnb1-2173-bsa-and-azide-free-ab237825--immunocytochemistry-immunofluorescence-img95214.jpg "Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (ab237825)")
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (ab237825)
Immunocytochemical/immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling beta Catenin (Green) using Anti-beta Catenin antibody [rCTNNB1/2173] ab237983 at 2 μg/mL. The nuclei are stained in red.
This data was produced with Anti-beta Catenin antibody [rCTNNB1/2173] ab237983, the same antibody in a different formulation with BSA and Azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (ab237825), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-rctnnb1-2173-bsa-and-azide-free-ab237825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129302.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (ab237825)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (ab237825)
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for beta Catenin antibody using Anti-beta Catenin antibody [rCTNNB1/2173] ab237983 at 2 μg/ml in immunohistochemical analysis.
This data was produced with Anti-beta Catenin antibody [rCTNNB1/2173] ab237983, the same antibody in a different formulation with BSA and Azide.
![Protein Array - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (ab237825), expandable thumbnail](https://content.abcam.com/products/images/beta-catenin-antibody-rctnnb1-2173-bsa-and-azide-free-ab237825--protein-array-img140158.jpg "Protein Array - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (ab237825)")
Protein Array - Anti-beta Catenin antibody [rCTNNB1/2173] - BSA and Azide free (ab237825)
This data was produced with Anti-beta Catenin antibody [rCTNNB1/2173] ab237983, the same antibody in a different formulation with BSA and Azide.
Anti-beta Catenin antibody [rCTNNB1/2173] ab237983 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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