AB75777

Anti-beta Catenin (phospho S37) antibody [EP742(2)Y]

RabMAb
Recombinant
Lab Essentials
20ul selling size
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(28 Publications)

Rabbit Recombinant Monoclonal beta Catenin phospho S37 antibody. Suitable for WB, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 28 publications.

View Alternative Names

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

6 Images

Lab

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa treated with 100nM Calyculin A for 30 min whole cell lysate at 15 µg

Lane 3:

HeLa treated with 100nM Calyculin A for 30 min whole cell lysate, then membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 85 kDa

Observed band size: 100 kDa

false

Exposure time: 80s

Lab

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/1000 dilution

Lane 1:

Untreated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

HEK-293 treated with 50nM Calyculin A for 3 hours whole cell lysate at 15 µg

Lane 3:

HEK-293 treated with 50nM Calyculin A for 3 hours whole cell lysate, then membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 85 kDa

Observed band size: 100 kDa

false

Exposure time: 10s

Lab

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/1000 dilution

Lane 1:

Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Lane 2:

C6 treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg

Lane 3:

C6 treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 85 kDa

Observed band size: 100 kDa

false

Exposure time: 40s

Lab

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 2:

NIH/3T3 treated with 100nM Calyculin A for 30 min whole cell lysate at 15 µg

Lane 3:

NIH/3T3 treated with 100nM Calyculin A for 30 min whole cell lysate, then membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 85 kDa

Observed band size: 100 kDa

false

Exposure time: 120s

Unknown

Dot Blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Dot blot analysis of Beta catenin phospho peptide with ab75777 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) (1/100000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.

Lane 1 : Beta catenin (pS33+pS37) phospho peptide
Lane 2 : Beta catenin (pS33) phospho peptide
Lane 3 : Beta catenin (pS37) phospho peptide
Lane 4 : Beta catenin non-phospho peptide

CiteAb

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

beta Catenin (phospho S37) western blot using anti-beta Catenin (phospho S37) antibody [EP742(2)Y] ab75777. Publication image and figure legend from Lin, J. X., Lian, N. Z., et al., 2022, Cell Death Dis, PubMed 35568711.

ab75777 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab75777 please see the product overview.

LHPP suppresses aerobic glycolysis.A Relative mRNA expression levels of glycolytic genes and glutamine transporters in LHPP-overexpressing or LHPP-knockdown MGC-803 cells and control cells by qPCR. B Protein expression levels of glycolytic genes and glutamine transporters in LHPP-overexpressing or LHPP-knockdown MGC-803 cells and control cells on western blot. C IHC staining of LHPP and HIF1A in TMAs and their correlation. Scale bars = 100 μm. D Oxygen consumption rate and extracellular acidification rate of LHPP-overexpressing or LHPP-knockdown MGC-803 cells and control cells were measured using the Seahorse Bioscience XF96 analyser. E Human phosphokinase microarray assay analysis of the conditioned medium from stably transfected HGC-27 cells. A summary of the relative signal intensities of the indicated proteins is shown. G, H IHC staining of LHPP and p-GSK3b in TMAs and their correlation. Scale bars = 200 μm. F Combined LHPP and GSK3b by co-immunoprecipitation. I Protein expression levels of the Wnt pathway in LHPP-overexpressing or LHPP-knockdown MGC-803 cells and control cells on western blot. Data were analyzed using the Wilcoxon test. *p < 0.05, **p < 0.01, ***p < 0.001, KEGG Kyoto encyclopaedia of genes and genomes, GO gene ontology, qPCR quantitative polymerase chain reaction, IHC immunohistochemical, TMA tissue microarray.

false

Carrier free

Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP742(2)Y

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.

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Reactivity data

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.

Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key downstream component of the canonical Wnt signaling pathway (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). Also acts as a coactivator for other transcription factors, such as NR5A2 (PubMed : 22187462). Promotes epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via driving transcription of CTNNB1/TCF-target genes (PubMed : 29910125). Involved in the regulation of cell adhesion, as component of an E-cadherin : catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed : 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed : 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed : 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed : 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9 : SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).

See full target information CTNNB1 phospho S37

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 13:2437-2450 PubMed38881929

2024

Up-regulation of NCAPG mediated by E2F1 facilitates the progression of osteosarcoma through the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gan Luo,Haiyang Cheng,Jigeng Fan,Tianwei Sun

RSC advances 14:1527-1537 PubMed38179095

2024

Enhancing osteogenic differentiation of MC3T3-E1 cells during inflammation using UPPE/β-TCP/TTC composites the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Lin Li,Ya-Xin Wu,Yu-Xiao Zhang,Jing Mao,Zhi-Xing Zhang

Journal of radiation research 64:880-892 PubMed37697698

2023

Therapeutic mechanism of Liangxue-Guyuan-Yishen decoction on intestinal stem cells and tight junction proteins in gastrointestinal acute radiation syndrome rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqiao Yan,Bofeng Yin,Yuguo Wang,Zhexin Ni,Jian Feng,Qianyu Yang,Xiao Li,Heng Zhu,Yongqi Dou

Biological research 56:48 PubMed37608294

2023

Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang-Shuang Cui,Ping Zhang,Lu Sun,Yu-Lin-Lan Yuan,Jingyun Wang,Feng-Xiang Zhang,Ruiman Li

International urology and nephrology 55:3065-3075 PubMed37594700

2023

Serpina3n/serpina3 alleviates cyclophosphamide-induced interstitial cystitis by activating the Wnt/β-catenin signal.

Applications

Unspecified application

Species

Unspecified reactive species

Weilin Fang,Qixiang Song,Tingting Lv,Jianwei Lv,Zhikang Cai,Zhong Wang,Xin Song,Xiang Ji,Jin Huang

Psychiatry investigation 19:771-787 PubMed36327957

2022

m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Ming,Zhihui Deng,Xianhua Tian,Yuerong Jia,Meng Ning,Shuhua Cheng

Molecular metabolism 64:101553 PubMed35863636

2022

Empagliflozin activates Wnt/β-catenin to stimulate FUNDC1-dependent mitochondrial quality surveillance against type-3 cardiorenal syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Cai,Feng Wu,Bingjie Zhuang,Qing Ou,Xiaojie Peng,Nengxian Shi,Lan Peng,Ziying Li,Jin Wang,Shumin Cai,Ying Tan

Cell death & disease 13:463 PubMed35568711

2022

m6A methylation mediates LHPP acetylation as a tumour aerobic glycolysis suppressor to improve the prognosis of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Xian Lin,Ning-Zi Lian,You-Xin Gao,Qiao-Ling Zheng,Ying-Hong Yang,Yu-Bin Ma,Zhi-Song Xiu,Qing-Zhu Qiu,Hua-Gen Wang,Chao-Hui Zheng,Ping Li,Jian-Wei Xie,Jun Lu,Qi-Yue Chen,Long-Long Cao,Mi Lin,Jia-Bin Wang,Chang-Ming Huang

Microbiology spectrum 10:e0273021 PubMed35377227

2022

Proteomic Response Revealed Signaling Pathways Involving in the Mechanism of Polymyxin B-Induced Melanogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chuhan Zhang,Xiaofen Liu,Hailan Wu,Yu Wang,Yaxin Fan,Beining Guo,Xingchen Bian,Xin Li,Jing Zhang

Molecular cancer 21:92 PubMed35366893

2022

Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Anqi Sun,Aihui Yan,Jian Yao,Haibo Huang,Ziming Gao,Tao Han,Jia Gu,Ni Li,Huizhe Wu,Kai Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Catenin (phospho S37) antibody [EP742(2)Y]

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Dot Blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (28)

Product promise