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Rabbit Recombinant Monoclonal beta Catenin phospho S37 antibody. Suitable for WB, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 19 publications.

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Images

Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777), expandable thumbnail
  • Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777), expandable thumbnail
  • Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777), expandable thumbnail
  • Dot Blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777), expandable thumbnail
  • Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (AB75777), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIPFlow CytDotIHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Expected
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Expected
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Expected
Not recommended
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).

Alternative names

Catenin beta-1, Beta-catenin, PRO2286, OK/SW-cl.35, CTNNB, CTNNB1

Recommended products

Rabbit Recombinant Monoclonal beta Catenin phospho S37 antibody. Suitable for WB, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 19 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP742(2)Y

Purification technique

Affinity purification Protein A

Specificity

Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.

Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Associated diseases and disorders

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777), expandable thumbnail

    Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/1000 dilution

    Lane 1: Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

    Lane 2: C6 treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 15 µg

    Lane 3: C6 treated with 100ng/ml Calyculin A for 30 min whole cell lysate, then membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa

    Observed band size: 100 kDa

    Exposure time: 40s

  • Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777), expandable thumbnail

    Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

    Lane 2: NIH/3T3 treated with 100nM Calyculin A for 30 min whole cell lysate at 15 µg

    Lane 3: NIH/3T3 treated with 100nM Calyculin A for 30 min whole cell lysate, then membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa

    Observed band size: 100 kDa

    Exposure time: 120s

  • Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777), expandable thumbnail

    Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/1000 dilution

    Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: HeLa treated with 100nM Calyculin A for 30 min whole cell lysate at 15 µg

    Lane 3: HeLa treated with 100nM Calyculin A for 30 min whole cell lysate, then membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa

    Observed band size: 100 kDa

    Exposure time: 80s

  • Dot Blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777), expandable thumbnail

    Dot Blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777)

    Dot blot analysis of Beta catenin phospho peptide with ab75777 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.

    Lane 1: Beta catenin (pS33+pS37) phospho peptide
    Lane 2: Beta catenin (pS33) phospho peptide
    Lane 3: Beta catenin (pS37) phospho peptide
    Lane 4: Beta catenin non-phospho peptide

  • Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777), expandable thumbnail

    Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/1000 dilution

    Lane 1: Untreated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

    Lane 2: HEK-293 treated with 50nM Calyculin A for 3 hours whole cell lysate at 15 µg

    Lane 3: HEK-293 treated with 50nM Calyculin A for 3 hours whole cell lysate, then membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa

    Observed band size: 100 kDa

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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