AB277785

Anti-beta Catenin (phospho S552) antibody [1HCLC]

Recombinant
Lab Essentials
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Multiclonal beta Catenin phospho S552 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CTNNB1 phospho S552 aa 500-600.

View Alternative Names

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho S552) antibody [1HCLC] (AB277785)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho S552) antibody [1HCLC] (AB277785)

For immunofluorescence analysis, SW480 cells were fixed and permeabilized for detection of endogenous Beta-Catenin pS552 using Anti-Beta-Catenin pS552 Recombinant Rabbit Multiclonal Antibody (ab277785, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal^™ Secondary Antibody, Alexa Fluor^® 488 conjugate (1/2000). Nuclei (blue) were stained using SlowFade^® Gold Antifade Mountant with DAPI, and Alexa Fluor^® 555 Rhodamine Phalloidin (1/300) was used for cytoskeletal F-actin (red) staining. Detection and localization of Beta-Catenin pS552 (green) in the cytoplasm can be clearly observed in cells treated with the proteasome inhibitor Mμ-132 (25 uM, 4 h) as compared to untreated cells. Antibody specificity was demonstrated by competition with the Beta-Catenin pS552 peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.

Supplier Data

Western blot - Anti-beta Catenin (phospho S552) antibody [1HCLC] (AB277785)

All lanes:

Western blot - Anti-beta Catenin (phospho S552) antibody [1HCLC] (ab277785) at 2 µg/mL

Lane 1:

SW480 (human colorectal adenocarcinoma cell line) whole cell extract

Lane 2:

SW480 (human colorectal adenocarcinoma cell line) whole cell extract (treated with MG132, 25 uM for 4 hrs)

Lane 3:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract

Lane 4:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract (treated with MG132, 25 uM for 4 hrs)

Lane 5:

MCF7 (human breast adenocarcinoma cell line) whole cell extract

Lane 6:

MCF7 (human breast adenocarcinoma cell line) whole cell extract (treated with MG132, 25 uM for 4 hrs)

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

1HCLC

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CTNNB1 phospho S552 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

P35222

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.

Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Key downstream component of the canonical Wnt signaling pathway (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin : catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed : 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed : 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed : 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed : 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9 : SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).

See full target information CTNNB1 phospho S552

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic translation 38:158-166 PubMed36439631

2022

AMPK activator decelerates osteoarthritis development by inhibition of β-catenin signaling in chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenglin Zhu,Yanran Huang,Jun Li,Dan Yi,Junyi Liao,Jun Xiao,Guozhi Xiao,Liping Tong,Wei Huang,Chen Di

BioMed research international 2022:9235837 PubMed36246971

2022

SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongbo Du,Xiaobin Chen,Pingyu Zhu,Wei Sun,Qi Lv,Shulin Cheng,Xuesong Yang,Xiaodong Yu

Biomolecules 12: PubMed36291598

2022

Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyang Xiao,Lijing Wang,Juling Zhang,Xinyu Zhong,Zhou Guo,Jiahao Yu,Yuanyuan Ma,Haigang Wu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Catenin (phospho S552) antibody [1HCLC]

Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho S552) antibody [1HCLC] (AB277785)

Western blot - Anti-beta Catenin (phospho S552) antibody [1HCLC] (AB277785)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise