AB314503

Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal beta Catenin phospho S552 antibody. Carrier free. Suitable for WB, Dot, IP and reacts with Human, Mouse, Synthetic peptide - Human samples.

View Alternative Names

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

6 Images

Supplier Data

Immunoprecipitation - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

This data was developed using ab314502, the same antibody clone in a different buffer formulation.

beta Catenin (phospho S552) was immunoprecipitated from 0.35 mg SK-N-MC (human brain epithelial cell) treated with 100 μM forskolin for 30 minutes whole cell lysate with ab314502 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314502 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SK-N-MC (human brain epithelial cell) treated with 100 μM forskolin for 30 minutes whole cell lysate

Lane 2 : ab314502 IP in SK-N-MC (human brain epithelial cell) treated with 100 μM forskolin for 30 minutes whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314502 in SK-N-MC treated with 100 μM forskolin for 30 minutes whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] (<a href='/en-us/products/primary-antibodies/beta-catenin-phospho-s552-antibody-epr28411-7-ab314502'>ab314502</a>) at 1/30 dilution

All lanes:

SK-N-MC (human brain epithelial cell) treated with 100 ?M forskolin for 30 minutes whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 145s

Supplier Data

Western blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

This data was developed using ab314502, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30806153).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Catenin beta-1 antibody - Total protein control (ab32572) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] (<a href='/en-us/products/primary-antibodies/beta-catenin-phospho-s552-antibody-epr28411-7-ab314502'>ab314502</a>) at 1/1000 dilution

Lane 1:

Untreated SK-N-MC (human brain epithelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

SK-N-MC treated with 100 uM forskolin for 30 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

SK-N-MC treated with 100 uM forskolin for 30 minutes whole cell lysate (Lambda phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 85 kDa,90 kDa,36 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

This data was developed using ab314502, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30806153).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Catenin beta-1 antibody - Total protein control (ab32572) staining at 1/1000 dilution.

All lanes:

Lane 1:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

SW480 whole cell lysate (Lambda phosphatase membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 85 kDa,90 kDa,36 kDa

true

Exposure time: 136s

Supplier Data

Western blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

This data was developed using ab314502, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30806153).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 85 kDa,90 kDa,36 kDa

false

Exposure time: 59s

Supplier Data

Western blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

This data was developed using ab314502, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30806153).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Catenin beta-1 antibody - Total protein control (ab32572) staining at 1/1000 dilution.

All lanes:

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) starved for 24 hours whole cell lysate (untreated membrane) at 20 µg

Lane 2:

NIH/3T3 starved for 24 hours, then treated with 100 uM forskolin for 20 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

NIH/3T3 starved for 24 hours, then treated with 100 uM forskolin for 20 minutes whole cell lysate (Lambda phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 85 kDa,90 kDa,36 kDa

false

Exposure time: 103s

Supplier Data

Dot Blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

This data was developed using ab314502, the same antibody clone in a different buffer formulation.

Dot blot analysis of beta Catenin (phospho S552) using ab314502 at 1 : 1000 (0.496 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with non-phospho gamma Catenin peptide covering aa 534-548.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28411-7

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, IP, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for mouse IP.

In western blot, we have observed a lower Mw band at ~55KDa in some normal colon tissue samples. We welcome any feedback from customers who have used this antibody in tissue.

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Reactivity data

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Product details

ab314503 is the carrier-free version of ab314502.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.

Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key downstream component of the canonical Wnt signaling pathway (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin : catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed : 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed : 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed : 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed : 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9 : SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).

See full target information CTNNB1 phospho S552

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free

Immunoprecipitation - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

Western blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

Western blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

Western blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

Western blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

Dot Blot - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] - BSA and Azide free (AB314503)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise