AB81305

Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y]

Name: Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y] (ab81305) | Abcam
Brand: Abcam Limited
SKU: AB81305
Price: 3600 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
Lab Essentials
20ul selling size
What is this?

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal beta Catenin phospho S45 + T41 antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications.

View Alternative Names

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

2 Images

Unknown

Western blot - Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y] (AB81305)

All lanes:

Western blot - Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y] (ab81305) at 1/20000 dilution

Lane 1:

293T cell lysates un-treated at 10 µg

Lane 2:

293T cell lysates treated with calyculin A at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 85 kDa

Observed band size: 70 kDa

false

CiteAb

Western blot - Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y] (AB81305)

beta Catenin (phospho T41 + S45) western blot using anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y] ab81305. Publication image and figure legend from Tong, Y. Q., Liu, B., et al., 2015, Oncotarget, PubMed 26317904.

ab81305 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab81305 please see the product overview.

Expression comparison of TGF-β1, CTNNBIP1, α-SMA, and Fibronectin in human glioma tumor tissue and adjacent normal tissueA–B. Expression of the indicated proteins in adjacent normal tissue and tumor tissues are showed in A by western blot and in B as quantitated data. C. CTNNBIP1 mRNA and miR-215 expression levels in the tumor tissue and adjacent normal tissue are examined using qPCR and their correlation was analyzed by the Pearson correlation method.

false

Carrier free

Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1905Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.

Biological function summary

This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.

Pathways

Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.

Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Key downstream component of the canonical Wnt signaling pathway (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed : 17524503, PubMed : 18077326, PubMed : 18086858, PubMed : 18957423, PubMed : 21262353, PubMed : 22155184, PubMed : 22647378, PubMed : 22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin : catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed : 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed : 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed : 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed : 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9 : SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).

See full target information CTNNB1 phospho S45 + T41

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

BioMed research international 2022:8638085 PubMed35978634

2022

Chalcone 9X Contributed to Repressing Glioma Cell Growth and Migration and Inducing Cell Apoptosis by Reducing FOXM1 Expression In Vitro and Repressing Tumor Growth In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Chenguang Li,Rui Wang,Wenshi Guo,Xu Feng,Ning Guan

Bioengineered 12:5688-5704 PubMed34546849

2021

α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Siwen Zhang,Junfu Wang,Ting Chen,Jiancheng Wang,Ye Wang,Zhu Yu,Kun Zhao,Kaitian Zheng,Yeyang Chen,Zhen Wang,Bopei Li,Congjun Wang,Weijia Huang,Zhao Fu,Junqiang Chen

Frontiers in medicine 8:667543 PubMed34434939

2021

mA RNA Methylation Regulator YTHDF1 Correlated With Immune Microenvironment Predicts Clinical Outcomes and Therapeutic Efficacy in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Hu,Qinwen Pan,Minghao Wang,Xiang Ai,Yuzhao Yan,Yuan Tian,Yuting Jing,Peng Tang,Jun Jiang

Oncology reports 46: PubMed34278502

2021

RORβ suppresses the stemness of gastric cancer cells by downregulating the activity of the Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Wen,Ming Chen,Wenhao Guo,Ke Guo,Ping Du,Yanfei Fang,Min Gao,Qiang Wang

Oncology reports : PubMed33469678

2021

JMJD2C triggers the growth of multiple myeloma cells via activation of β‑catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Lv,Qicai Liu

Journal of experimental & clinical cancer research 39:202 PubMed32993749

2020

FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2.

Applications

Unspecified application

Species

Unspecified reactive species

Pin Guo,Zibin Tian,Xinjuan Kong,Lin Yang,Xinzhi Shan,Bingzi Dong,Xueli Ding,Xue Jing,Chen Jiang,Na Jiang,Yanan Yu

Molecules (Basel, Switzerland) 24: PubMed31212829

2019

Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Loris Bertazza,Susi Barollo,Maria Elena Mari,Irene Faccio,Maira Zorzan,Marco Redaelli,Beatrice Rubin,Decio Armanini,Caterina Mian,Raffaele Pezzani

Chinese medicine 11:45 PubMed27733866

2016

Inhibitory effect of -ferulic acid on proliferation and migration of human lung cancer cells accompanied with increased endogenous reactive oxygen species and β-catenin instability.

Applications

Species

Human

Yao Fong,Chia-Chun Tang,Huei-Ting Hu,Hsin-Yu Fang,Bing-Hung Chen,Chang-Yi Wu,Shyng-Shiou Yuan,Hui-Min David Wang,Yen-Chun Chen,Yen-Ni Teng,Chien-Chih Chiu

Oncotarget 6:25024-33 PubMed26317904

2015

MiR-215, an activator of the CTNNBIP1/β-catenin pathway, is a marker of poor prognosis in human glioma.

Applications

Species

Unspecified reactive species

Yong-Qing Tong,Bei Liu,Hong-Yun Zheng,Jian Gu,Hang Liu,Feng Li,Bi-Hua Tan,Melanie Hartman,Chunhua Song,Yan Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y]

Western blot - Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y] (AB81305)

Western blot - Anti-beta Catenin (phospho T41 + S45) antibody [EP1905Y] (AB81305)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise