Rabbit Polyclonal beta Catenin phospho Y142 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 62 publications. Immunogen corresponding to Synthetic Peptide within Human CTNNB1 phospho Y142 conjugated to Keyhole Limpet Haemocyanin.
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- Oxidative medicine and cellular longevity 2023:70983132023NF-B Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhou,Wenxuan Qian,Xiangming Li,Wenzhi WeiPubMed 36699318
- PeerJ 10:e142632022GPX2 predicts recurrence-free survival and triggers the Wnt/β-catenin/EMT pathway in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Yang,Xudong Zhu,Yang Shen,Qi He,Yuan Qin,Yiqun Shao,Lin Yuan,Hesong YePubMed 36312753
- Frontiers in oncology 12:8052902022Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeitao Zhang,Lu Kong,Hongbin Zhu,Decong Sun,Quanli Han,Bin Yan,Zhi Cui,Weiwei Zhang,Shurong Zhang,Xindan Kang,Guanghai Dai,Niansong Qian,Wenji YanPubMed 35299743
- Journal of oncology 2022:23523602022Overexpression of PTPRZ1 Regulates p120/-Catenin Phosphorylation to Promote Carcinogenesis of Oral Submucous Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiwei Ma,Ting Shen,Hui Peng,Jianjun Wu,Wenjin Wang,Xing GaoPubMed 35251170
- Journal of experimental & clinical cancer research : CR 41:702022FOXQ1-mediated SIRT1 upregulation enhances stemness and radio-resistance of colorectal cancer cells and restores intestinal microbiota function by promoting β-catenin nuclear translocation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei Yang,Qian Liu,Maolin Dai,Renqun Peng,Xinghui Li,Wei Zuo,Juhua Gou,Feixue Zhou,Shuangjiang Yu,Hao Liu,Min HuangPubMed 35183223
- Frontiers in endocrinology 12:8078822022Accelerated Development With Increased Bone Mass and Skeletal Response to Loading Suggest Receptor Activity Modifying Protein-3 as a Bone Anabolic Target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuruchi Pacharne,Matthew Livesey,Mahita Kadmiel,Ning Wang,Kathleen M Caron,Gareth O Richards,Tim M SkerryPubMed 35095771
- Molecular therapy. Nucleic acids 27:122-1322022rAAV-delivered PTEN therapeutics for prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianzhong Ai,Jia Li,Qin Su,Hong Ma,Qiang Wei,Hong Li,Guangping GaoPubMed 34976432
- Scientific reports 11:209482021Wnt6 plays a complex role in maintaining human limbal stem/progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClémence Bonnet,Denise Oh,Hua Mei,Sarah Robertson,Derek Chang,Jean-Louis Bourges,Francine Behar-Cohen,Jie J Zheng,Sophie X DengPubMed 34686698
- Cancer medicine 10:4030-40452021IBSP, a potential recurrence biomarker, promotes the progression of colorectal cancer via Fyn/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Chen,Ying Qin,Mengmeng Dai,Liping Liu,Yong Ni,Qinsheng Sun,Lulu Li,Yaoyao Zhou,Cheng Qiu,Yuyang JiangPubMed 33987980
- Frontiers in neurology 12:6190432021Electroacupuncture Ameliorates Cerebral Ischemic Injury by Inhibiting Ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangda Li,Xiaoxiao Li,Jianjian Dong,Yongsheng HanPubMed 33763013
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CTNNB1 phospho Y142 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P35222
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes IP with beta catenin antibody is recommended, followed by probing with ab27798. This improve the distinction between beta Catenin Tyr 142 and gamma Catenin Tyr 133 (Swiss-Prot ID P14923). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4 (By similarity). Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts (By similarity).
Alternative names
CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin
Recommended products
Rabbit Polyclonal beta Catenin phospho Y142 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 62 publications. Immunogen corresponding to Synthetic Peptide within Human CTNNB1 phospho Y142 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CTNNB1 phospho Y142 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P35222
- Purification technique
- Affinity purification Immunogen
- Specificity
The antibody detects beta Catenin on SDS PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells. Similar results were observed in other pervanadate treated cell lines, such as A431 and human endothelial cells.
Immunogen sequence has one amino acid difference from a sequence around tyrosine 133 of human gamma Catenin. These human sequences are highly conserved in rat and mouse beta Catenins. Batches could vary in the level phospho Y133 of gamma catenin recognition.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Tyrosine phosphorylation of beta Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro and overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta Catenins. The phosphorylation of tyrosine 142 may act as a switch from the adhesive to the transcriptional role of beta Catenin. Thus, site specific tyrosine phosphorylation of beta Catenin may regulate specific protein protein interactions, leading to changes in cell adhesion.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.
- Biological function summary
This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.
- Pathways
Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.
- Associated diseases and disorders
Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-beta Catenin (phospho Y142) antibody (ab27798)
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Lanes 1 - 2: Western blot - Anti-beta Catenin (phospho Y142) antibody (ab27798)
Lanes 3 - 4: Western blot - Anti-beta Catenin (phospho Y142) antibody (ab27798) at 1/500 dilution
Lanes 1 and 3: Hct116 src transformed cells serum starved overnight. at 20 µg
Lanes 2 and 4: Hct116 src transformed cells treated with pervanadate (1 mM) for 30 min. at 20 µg
Predicted band size: 85 kDa
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798)
ICC/IF labeling of phosphorylated β-Catenin in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal β-Catenin (left panel) and ab27798 antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Image courtesy of a customer review submitted by Judit Herreros.Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798)
ab27798 staining beta Catenin (phospho Y142) in rat hippocampal neurons by Immunocytochemistry/ Immunofluorescence. The cells were pre-treated with pervanadate for 15 minutes before being fixed in paraformaldehyde and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 8 hours at 4°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150076) used at a 1/500 dilution. Hoestch was used to stain the cell nuclei (blue).
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