AB151713

Anti-beta Crystallin A3 antibody [EPR9585]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal beta Crystallin A3 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CRYB1, CRYBA1, Beta-crystallin A3

1 Images

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Western blot - Anti-beta Crystallin A3 antibody [EPR9585] (AB151713)

All lanes:

Western blot - Anti-beta Crystallin A3 antibody [EPR9585] (ab151713) at 1/1000 dilution

Lane 1:

Mouse eyeball lysate at 10 µg

Lane 2:

Rat eyeball lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9585

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Crystallin A3 also known as CRYBA3 is a protein involved in the structural formation of the vertebrate eye lens. This protein belongs to the beta/gamma crystallin family characterized by double Greek key motifs. Beta Crystallin A3 has a molecular mass of approximately 24 kDa. It is synthesized in the lens fibers where it plays a role in maintaining transparency and refractive properties essential for vision. Other tissues may express it at much lower levels indicating its specific function in the lens.

Biological function summary

The protein plays a critical role in the formation of the lens's refractive index. It is not part of a complex but exists as a monomer unlike some other crystallins that form oligomers. Its function is to contribute to the lens's structural stability and its ability to focus light. The consistent production and maintenance of this protein are necessary to ensure the lens’s optical clarity over time which is vital for proper vision.

Pathways

Beta crystallin A3 interplays with stabilization pathways related to protein folding within the lens. It is connected to the cytoskeletal pathway which helps maintain the lens's shape and structure. Proteins such as alpha crystallins acting as chaperones interact with beta crystallins to prevent aggregation and maintain solubility in the dense environment of the lens.

Mutations in beta crystallin A3 associate with cataract formation in humans which leads to lens opacities and vision impairment. Cataracts are often linked with disruptions in protein folding and solubility. In this context the protein’s relationship with alpha crystallin is significant because alpha crystallin's chaperone activity can mitigate aggregation issues that lead to cataracts. Understanding these protein interactions helps develop therapeutic strategies to prevent or treat lens opacity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Crystallins are the dominant structural components of the vertebrate eye lens.

See full target information CRYBA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 16:915969 PubMed35669106

2022

Ablation of GSDMD Attenuates Neurological Deficits and Neuropathological Alterations After Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Du,Chang-Hong Li,Ruo-Bing Gao,Xiao-Qing Cen,Ping Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Crystallin A3 antibody [EPR9585]

Western blot - Anti-beta Crystallin A3 antibody [EPR9585] (AB151713)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise