AB151722

Anti-beta Crystallin A3 antibody [EPR9586]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(9 Publications)

Rabbit Recombinant Monoclonal beta Crystallin A3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

View Alternative Names

CRYB1, CRYBA1, Beta-crystallin A3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta Crystallin A3 antibody [EPR9586] (AB151722)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta Crystallin A3 antibody [EPR9586] (AB151722)

Immunofluorescent analysis of Y79 cells labeling beta Crystallin A3 with ab151722 at 1/50 dilution.

Unknown

Western blot - Anti-beta Crystallin A3 antibody [EPR9586] (AB151722)

All lanes:

Western blot - Anti-beta Crystallin A3 antibody [EPR9586] (ab151722) at 1/10000 dilution

Lane 1:

Mouse eyeball lysate at 10 µg

Lane 2:

Rat eyeball lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 25 kDa

false

Carrier free

Anti-beta Crystallin A3 antibody [EPR9586] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9586

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Crystallin A3 also known as CRYBA3 is a protein involved in the structural formation of the vertebrate eye lens. This protein belongs to the beta/gamma crystallin family characterized by double Greek key motifs. Beta Crystallin A3 has a molecular mass of approximately 24 kDa. It is synthesized in the lens fibers where it plays a role in maintaining transparency and refractive properties essential for vision. Other tissues may express it at much lower levels indicating its specific function in the lens.

Biological function summary

The protein plays a critical role in the formation of the lens's refractive index. It is not part of a complex but exists as a monomer unlike some other crystallins that form oligomers. Its function is to contribute to the lens's structural stability and its ability to focus light. The consistent production and maintenance of this protein are necessary to ensure the lens’s optical clarity over time which is vital for proper vision.

Pathways

Beta crystallin A3 interplays with stabilization pathways related to protein folding within the lens. It is connected to the cytoskeletal pathway which helps maintain the lens's shape and structure. Proteins such as alpha crystallins acting as chaperones interact with beta crystallins to prevent aggregation and maintain solubility in the dense environment of the lens.

Mutations in beta crystallin A3 associate with cataract formation in humans which leads to lens opacities and vision impairment. Cataracts are often linked with disruptions in protein folding and solubility. In this context the protein’s relationship with alpha crystallin is significant because alpha crystallin's chaperone activity can mitigate aggregation issues that lead to cataracts. Understanding these protein interactions helps develop therapeutic strategies to prevent or treat lens opacity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Crystallins are the dominant structural components of the vertebrate eye lens.

See full target information CRYBA1

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 24:e70018 PubMed39957408

2025

Activated mTOR Signaling in the RPE Drives EMT, Autophagy, and Metabolic Disruption, Resulting in AMD-Like Pathology in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia Chowdhury,Sridhar Bammidi,Pooja Gautam,Vishnu Suresh Babu,Haitao Liu,Peng Shang,Ying Xin,Emma Mahally,Mihir Nemani,Victoria Koontz,Kira Lathrop,Katarzyna M Kedziora,Jonathan Franks,Ming Sun,Joshua W Smith,Lauren R DeVine,Robert N Cole,Nadezda Stepicheva,Anastasia Strizhakova,Sreya Chattopadhyay,Stacey Hose,Jacob Samuel Zigler,José-Alain Sahel,Jiang Qian,Prasun Guha,James T Handa,Sayan Ghosh,Debasish Sinha

Investigative ophthalmology & visual science 65:4 PubMed38558092

2024

Dysregulation of Autophagy Occurs During Congenital Cataract Development in βA3ΔG91 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Akosua K Boateng,Roy Joseph,Om P Srivastava

The Journal of biological chemistry 299:102965 PubMed36736424

2023

The second extracellular domain of connexin 50 is important for in cell adhesion, lens differentiation, and adhesion molecule expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Li,Yumeng Quan,Guangyan Wang,Bo Ma,Sumin Gu,Jean X Jiang

Communications biology 4:850 PubMed34239035

2021

βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Shang,Nadezda Stepicheva,Kenneth Teel,Austin McCauley,Christopher Scott Fitting,Stacey Hose,Rhonda Grebe,Meysam Yazdankhah,Sayan Ghosh,Haitao Liu,Anastasia Strizhakova,Joseph Weiss,Imran A Bhutto,Gerard A Lutty,Ashwath Jayagopal,Jiang Qian,José-Alain Sahel,J Samuel Zigler,James T Handa,Yuri Sergeev,Raju V S Rajala,Simon Watkins,Debasish Sinha

Communications biology 4:248 PubMed33627831

2021

βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity.

Applications

Unspecified application

Species

Unspecified reactive species

Sayan Ghosh,Haitao Liu,Meysam Yazdankhah,Nadezda Stepicheva,Peng Shang,Tanuja Vaidya,Stacey Hose,Urvi Gupta,Michael Joseph Calderon,Ming-Wen Hu,Archana Padmanabhan Nair,Joseph Weiss,Christopher S Fitting,Imran A Bhutto,Santosh Gopi Krishna Gadde,Naveen Kumar Naik,Chaitra Jaydev,Gerard A Lutty,James T Handa,Ashwath Jayagopal,Jiang Qian,José-Alain Sahel,Dhivyaa Rajasundaram,Yuri Sergeev,J Samuel Zigler,Swaminathan Sethu,Simon Watkins,Arkasubhra Ghosh,Debasish Sinha

Experimental eye research 196:108061 PubMed32387618

2020

CXCR5/NRF2 double knockout mice develop retinal degeneration phenotype at early adult age.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Huang,Anton Lennikov

Frontiers in immunology 10:1903 PubMed31474986

2019

Autoimmune-Mediated Retinopathy in CXCR5-Deficient Mice as the Result of Age-Related Macular Degeneration Associated Proteins Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Anton Lennikov,Madhu Sudhana Saddala,Anthony Mukwaya,Shibo Tang,Hu Huang

Investigative ophthalmology & visual science 59:AMD104-AMD113 PubMed30098172

2018

A Role for βA3/A1-Crystallin in Type 2 EMT of RPE Cells Occurring in Dry Age-Related Macular Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Sayan Ghosh,Peng Shang,Hiroto Terasaki,Nadezda Stepicheva,Stacey Hose,Meysam Yazdankhah,Joseph Weiss,Taiji Sakamoto,Imran A Bhutto,Shuli Xia,J Samuel Zigler,Ram Kannan,Jiang Qian,James T Handa,Debasish Sinha

Stem cells translational medicine 6:490-501 PubMed28191760

2017

Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Parul Choudhary,Heather Booth,Alex Gutteridge,Beata Surmacz,Irene Louca,Juliette Steer,Julie Kerby,Paul John Whiting

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Crystallin A3 antibody [EPR9586]

Immunocytochemistry/ Immunofluorescence - Anti-beta Crystallin A3 antibody [EPR9586] (AB151722)

Western blot - Anti-beta Crystallin A3 antibody [EPR9586] (AB151722)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise