Rabbit Recombinant Monoclonal beta Defensin 1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-beta Defensin 1 antibody [EPR23254-49] - BSA and Azide free (Capture) (AB273555), expandable thumbnail](https://content.abcam.com/products/images/beta-defensin-1-antibody-epr23254-49-bsa-and-azide-free-capture-ab273555--sandwich-elisa-img427684.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-beta Defensin 1 antibody [EPR23254-49] - BSA and Azide free (Capture) (AB273555)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:25122636).
Alternative names
BD1, HBD1, DEFB1, Beta-defensin 1, BD-1, hBD-1
Recommended products
Rabbit Recombinant Monoclonal beta Defensin 1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23254-49
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab273555 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human DEFB1. The recommended pair for sandwich ELISA is:
Capture: ab273555, Human DEFB1 Capture Antibody (unconjugated)
Detector: Anti-beta Defensin 1 antibody [EPR23254-179] - BSA and Azide free (Detector) ab273553, Human DEFB1 Detector Antibody (unconjugated)
The reference range value is 7.81-500 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Defensin 1 also known as DEFb-1 is an antimicrobial peptide with a mass of approximately 4.8 kDa. This defensin emits from epithelial cells across various tissues such as the respiratory tract urogenital tract and skin. It forms part of a family of small cysteine-rich cationic proteins. The defensin microenvironment allows it to disrupt microbial cell membranes providing immediate defense against pathogens.
- Biological function summary
This defensin protein plays an important role in the innate immune system by protecting epithelial surfaces from infection. DEFb-1 does not act as part of a larger protein complex and it independently modulates immune responses. Its expression helps maintain a balanced relationship between the host's immune system and microbial populations reducing inflammatory reactions.
- Pathways
The action of beta Defensin 1 integrates into the Toll-like receptor signaling and NF-kB pathways. It participates in regulating the immune response against microbial invasion. These pathways involve interactions with proteins like MyD88 and TRAF6 linking beta Defensin 1 to the broader network of innate immunity.
- Associated diseases and disorders
DEFb-1 has associations with conditions such as inflammatory bowel disease and cystic fibrosis. In inflammatory bowel disease the reduced expression of beta Defensin 1 can disrupt gut microbiota balance while in cystic fibrosis altered defensin levels affect mucus-associated bacterial load. The interplay between DEFb-1 and the CFTR protein highlights its impact on surface clearance and infection susceptibility.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-beta Defensin 1 antibody [EPR23254-49] - BSA and Azide free (Capture) (ab273555), expandable thumbnail](https://content.abcam.com/products/images/beta-defensin-1-antibody-epr23254-49-bsa-and-azide-free-capture-ab273555--sandwich-elisa-img427684.jpg "Sandwich ELISA - Anti-beta Defensin 1 antibody [EPR23254-49] - BSA and Azide free (Capture) (ab273555)")
Sandwich ELISA - Anti-beta Defensin 1 antibody [EPR23254-49] - BSA and Azide free (Capture) (ab273555)
Sandwich ELISA of Human beta Defensin 1 Antibody Pair - BSA and Azide free ab253535 with the capture antibody ab273555 dilution at 2 µg/mL and detector antibody Anti-beta Defensin 1 antibody [EPR23254-179] - BSA and Azide free (Detector) ab273553 dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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