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AB249627

Anti-beta Defensin 1 antibody [EPR6964(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal beta Defensin 1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

BD1, HBD1, DEFB1, Beta-defensin 1, BD-1, hBD-1

1 Images
Western blot - Anti-beta Defensin 1 antibody [EPR6964(2)] - BSA and Azide free (AB249627)
  • WB

Supplier Data

Western blot - Anti-beta Defensin 1 antibody [EPR6964(2)] - BSA and Azide free (AB249627)

This data was developed using ab170962, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-beta Defensin 1 antibody [EPR6964(2)] (<a href='/en-us/products/primary-antibodies/beta-defensin-1-antibody-epr69642-ab170962'>ab170962</a>) at 1/1000 dilution

All lanes:

Human recombinant beta Defensin 1 (10ng)

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 7 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6964(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249627 is the carrier-free version of ab170962.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Defensin 1 also known as DEFb-1 is an antimicrobial peptide with a mass of approximately 4.8 kDa. This defensin emits from epithelial cells across various tissues such as the respiratory tract urogenital tract and skin. It forms part of a family of small cysteine-rich cationic proteins. The defensin microenvironment allows it to disrupt microbial cell membranes providing immediate defense against pathogens.
Biological function summary

This defensin protein plays an important role in the innate immune system by protecting epithelial surfaces from infection. DEFb-1 does not act as part of a larger protein complex and it independently modulates immune responses. Its expression helps maintain a balanced relationship between the host's immune system and microbial populations reducing inflammatory reactions.

Pathways

The action of beta Defensin 1 integrates into the Toll-like receptor signaling and NF-kB pathways. It participates in regulating the immune response against microbial invasion. These pathways involve interactions with proteins like MyD88 and TRAF6 linking beta Defensin 1 to the broader network of innate immunity.

DEFb-1 has associations with conditions such as inflammatory bowel disease and cystic fibrosis. In inflammatory bowel disease the reduced expression of beta Defensin 1 can disrupt gut microbiota balance while in cystic fibrosis altered defensin levels affect mucus-associated bacterial load. The interplay between DEFb-1 and the CFTR protein highlights its impact on surface clearance and infection susceptibility.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed : 25122636).
See full target information DEFB1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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