Mouse Monoclonal beta Defensin 1 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human DEFB1 aa 1-50.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Defensin 1 antibody [M11-14b-D10] (AB14425), expandable thumbnail](https://content.abcam.com/products/images/beta-defensin-1-antibody-m11-14b-d10-ab14425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17471.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Defensin 1 antibody [M11-14b-D10] (AB14425)")
Publications
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- Journal of inflammation research 15:141-1522022Tumor-Infiltrating CD4 Central Memory T Cells Correlated with Favorable Prognosis in Oral Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Wu,Tianyi Zhang,Haofeng Xiong,Liujun Zeng,Zijia Wang,Ying Peng,Weijun Chen,Xin Hu,Tong SuPubMed 35035226
- PloS one 14:e02107022019Pelargonium sidoides radix extract EPs 7630 reduces rhinovirus infection through modulation of viral binding proteins on human bronchial epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Roth et. al.PubMed 30707726
- Scientific reports 8:180432018Expression of the human antimicrobial peptide β-defensin-1 is repressed by the EGFR-ERK-MYC axis in colonic epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClément Bonamy,Emmanuel Sechet,Aurélien Amiot,Antoine Alam,Michael Mourez,Laurent Fraisse,Philippe J Sansonetti,Brice SperandioPubMed 30575780
- The Journal of allergy and clinical immunology 143:173-181.e102018Loss-of-function mutations in caspase recruitment domain-containing protein 14 (CARD14) are associated with a severe variant of atopic dermatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlon Peled,Ofer Sarig,Guangping Sun,Liat Samuelov,Chi A Ma,Yuan Zhang,Tom Dimaggio,Celeste G Nelson,Kelly D Stone,Alexandra F Freeman,Liron Malki,Lucia Seminario Vidal,Latha M Chamarthy,Valeria Briskin,Janan Mohamad,Mor Pavlovsky,Jolan E Walter,Joshua D Milner,Eli SprecherPubMed 30248356
- International journal of pediatric otorhinolaryngo 110:100-1042018Tonsillar antimicrobial peptide (AMP) expression profiles of periodic fever, aphthous stomatitis, pharyngitis, cervical adenitis (PFAPA) patients.
- PloS one 12:e01880102017Broncho Vaxom (OM-85) modulates rhinovirus docking proteins on human airway epithelial cells via Erk1/2 mitogen activated protein kinase and cAMP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Roth et. al.PubMed 29182620
- Cellular physiology and biochemistry : internation 36:457-732015Cigarette Smoke Modulates NOD1 Signal Pathway and Human β Defensins Expression in Human Oral Mucosa.
- PloS one 10:e01170382015Antimicrobial protein and Peptide concentrations and activity in human breast milk consumed by preterm infants at risk of late-onset neonatal sepsis.Applications:ELISAReactive species:Unspecified reactive speciesStephanie Trend et. al.PubMed 25643281
- PloS one 9:e918672014Human beta-defensin-1 suppresses tumor migration and invasion and is an independent predictor for survival of oral squamous cell carcinoma patients.
- Journal of proteomics 75:1472-852011Proteinase 3 carries small unusual carbohydrates and associates with αlpha-defensins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMorten Zoega et. al.PubMed 22138257
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 0.79% Tris HCl- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human DEFB1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P60022
Reactivity data
Select an application| ELISA | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:25122636).
Alternative names
BD1, HBD1, DEFB1, Beta-defensin 1, BD-1, hBD-1
Recommended products
Mouse Monoclonal beta Defensin 1 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human DEFB1 aa 1-50.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 0.79% Tris HCl- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human DEFB1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P60022
- Clone number
- M11-14b-D10
- Purification technique
- Affinity purification Protein L
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Defensin 1 also known as DEFb-1 is an antimicrobial peptide with a mass of approximately 4.8 kDa. This defensin emits from epithelial cells across various tissues such as the respiratory tract urogenital tract and skin. It forms part of a family of small cysteine-rich cationic proteins. The defensin microenvironment allows it to disrupt microbial cell membranes providing immediate defense against pathogens.
- Biological function summary
This defensin protein plays an important role in the innate immune system by protecting epithelial surfaces from infection. DEFb-1 does not act as part of a larger protein complex and it independently modulates immune responses. Its expression helps maintain a balanced relationship between the host's immune system and microbial populations reducing inflammatory reactions.
- Pathways
The action of beta Defensin 1 integrates into the Toll-like receptor signaling and NF-kB pathways. It participates in regulating the immune response against microbial invasion. These pathways involve interactions with proteins like MyD88 and TRAF6 linking beta Defensin 1 to the broader network of innate immunity.
- Associated diseases and disorders
DEFb-1 has associations with conditions such as inflammatory bowel disease and cystic fibrosis. In inflammatory bowel disease the reduced expression of beta Defensin 1 can disrupt gut microbiota balance while in cystic fibrosis altered defensin levels affect mucus-associated bacterial load. The interplay between DEFb-1 and the CFTR protein highlights its impact on surface clearance and infection susceptibility.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Defensin 1 antibody [M11-14b-D10] (ab14425), expandable thumbnail](https://content.abcam.com/products/images/beta-defensin-1-antibody-m11-14b-d10-ab14425--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17471.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Defensin 1 antibody [M11-14b-D10] (ab14425)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Defensin 1 antibody [M11-14b-D10] (ab14425)
ab14425 (2µg/ml) staining beta defensin in human kidney, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining in the epithelial layers of the loops of Henle, distal tubules and collecting ducts.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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