AB14425

Anti-beta Defensin 1 antibody [M11-14b-D10]

Name: Anti-beta Defensin 1 antibody [M11-14b-D10] (ab14425) | Abcam
Brand: Abcam Limited
SKU: AB14425

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(10 Publications)

Mouse Monoclonal beta Defensin 1 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human DEFB1 aa 1-50.

View Alternative Names

BD1, HBD1, DEFB1, Beta-defensin 1, BD-1, hBD-1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Defensin 1 antibody [M11-14b-D10] (AB14425)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Defensin 1 antibody [M11-14b-D10] (AB14425)

ab14425 (2µg/ml) staining beta defensin in human kidney, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining in the epithelial layers of the loops of Henle, distal tubules and collecting ducts.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M11-14b-D10

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, ELISA, WB

applications

Immunogen

Synthetic Peptide within Human DEFB1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P60022

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "" }, "Chimpanzee": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein L

Storage buffer

pH: 7.4 Constituents: 0.79% Tris HCl

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Defensin 1 also known as DEFb-1 is an antimicrobial peptide with a mass of approximately 4.8 kDa. This defensin emits from epithelial cells across various tissues such as the respiratory tract urogenital tract and skin. It forms part of a family of small cysteine-rich cationic proteins. The defensin microenvironment allows it to disrupt microbial cell membranes providing immediate defense against pathogens.

Biological function summary

This defensin protein plays an important role in the innate immune system by protecting epithelial surfaces from infection. DEFb-1 does not act as part of a larger protein complex and it independently modulates immune responses. Its expression helps maintain a balanced relationship between the host's immune system and microbial populations reducing inflammatory reactions.

Pathways

The action of beta Defensin 1 integrates into the Toll-like receptor signaling and NF-kB pathways. It participates in regulating the immune response against microbial invasion. These pathways involve interactions with proteins like MyD88 and TRAF6 linking beta Defensin 1 to the broader network of innate immunity.

DEFb-1 has associations with conditions such as inflammatory bowel disease and cystic fibrosis. In inflammatory bowel disease the reduced expression of beta Defensin 1 can disrupt gut microbiota balance while in cystic fibrosis altered defensin levels affect mucus-associated bacterial load. The interplay between DEFb-1 and the CFTR protein highlights its impact on surface clearance and infection susceptibility.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed : 25122636).

See full target information DEFB1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 15:141-152 PubMed35035226

2022

Tumor-Infiltrating CD4 Central Memory T Cells Correlated with Favorable Prognosis in Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wu,Tianyi Zhang,Haofeng Xiong,Liujun Zeng,Zijia Wang,Ying Peng,Weijun Chen,Xin Hu,Tong Su

PloS one 14:e0210702 PubMed30707726

2019

Pelargonium sidoides radix extract EPs 7630 reduces rhinovirus infection through modulation of viral binding proteins on human bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Roth,Lei Fang,Daiana Stolz,Michael Tamm

Scientific reports 8:18043 PubMed30575780

2018

Expression of the human antimicrobial peptide β-defensin-1 is repressed by the EGFR-ERK-MYC axis in colonic epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Clément Bonamy,Emmanuel Sechet,Aurélien Amiot,Antoine Alam,Michael Mourez,Laurent Fraisse,Philippe J Sansonetti,Brice Sperandio

The Journal of allergy and clinical immunology 143:173-181.e10 PubMed30248356

2018

Loss-of-function mutations in caspase recruitment domain-containing protein 14 (CARD14) are associated with a severe variant of atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Alon Peled,Ofer Sarig,Guangping Sun,Liat Samuelov,Chi A Ma,Yuan Zhang,Tom Dimaggio,Celeste G Nelson,Kelly D Stone,Alexandra F Freeman,Liron Malki,Lucia Seminario Vidal,Latha M Chamarthy,Valeria Briskin,Janan Mohamad,Mor Pavlovsky,Jolan E Walter,Joshua D Milner,Eli Sprecher

International journal of pediatric otorhinolaryngo 110:100-104 PubMed29859567

2018

Tonsillar antimicrobial peptide (AMP) expression profiles of periodic fever, aphthous stomatitis, pharyngitis, cervical adenitis (PFAPA) patients.

Applications

IHC-P

Species

Human

Umut Gazi,Martha Emmanuel Agada,Hanife Ozkayalar,Ceyhun Dalkan,Burcin Sanlidag,Mustafa Asım Safak,Gamze Mocan,Nerin Onder Bahceciler

PloS one 12:e0188010 PubMed29182620

2017

Broncho Vaxom (OM-85) modulates rhinovirus docking proteins on human airway epithelial cells via Erk1/2 mitogen activated protein kinase and cAMP.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Roth,Christian Pasquali,Daiana Stolz,Michael Tamm

Cellular physiology and biochemistry : internation 36:457-73 PubMed25968832

2015

Cigarette Smoke Modulates NOD1 Signal Pathway and Human β Defensins Expression in Human Oral Mucosa.

Applications

Unspecified application

Species

Human

Ya-Jie Qian,Xiang Wang,Ya-Fan Gao,Ning Duan,Xiao-Feng Huang,Fang-Fang Sun,Xiao-Dong Han,Wen-Mei Wang

PloS one 10:e0117038 PubMed25643281

2015

Antimicrobial protein and Peptide concentrations and activity in human breast milk consumed by preterm infants at risk of late-onset neonatal sepsis.

Applications

ELISA

Species

Unspecified reactive species

Stephanie Trend,Tobias Strunk,Julie Hibbert,Chooi Heen Kok,Guicheng Zhang,Dorota A Doherty,Peter Richmond,David Burgner,Karen Simmer,Donald J Davidson,Andrew J Currie

PloS one 9:e91867 PubMed24658581

2014

Human beta-defensin-1 suppresses tumor migration and invasion and is an independent predictor for survival of oral squamous cell carcinoma patients.

Applications

IHC

Species

Human

Qi Han,Ruinan Wang,Chongkui Sun,Xin Jin,Dongjuan Liu,Xin Zhao,Lili Wang,Ning Ji,Jing Li,Yu Zhou,Ling Ye,Xinhua Liang,Lu Jiang,Ga Liao,Hongxia Dan,Xin Zeng,Qianming Chen

Journal of proteomics 75:1472-85 PubMed22138257

2011

Proteinase 3 carries small unusual carbohydrates and associates with αlpha-defensins.

Applications

Unspecified application

Species

Unspecified reactive species

Morten Zoega,Tina Ravnsborg,Peter Højrup,Gunnar Houen,Christian Schou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta Defensin 1 antibody [M11-14b-D10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Defensin 1 antibody [M11-14b-D10] (AB14425)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise