Rabbit Polyclonal DAG1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.
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- Scientific reports 12:31862022Sex-specific differences in KCC2 localisation and inhibitory synaptic transmission in the rat hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniele C Wolf,Nathalie T Sanon,Alexandra O S Cunha,Jia-Shu Chen,Tarek Shaker,Abdul-Rahman Elhassan,Antônia Sâmia Fernandes do Nascimento,Graziella Di Cristo,Alexander G WeilPubMed 35210456
- The Journal of physiology 597:4831-48502019Muscle specific kinase protects dystrophic mdx mouse muscles from eccentric contraction-induced loss of force-producing capacity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Trajanovska,J Ban,J Huang,P Gregorevic,M Morsch,D G Allen,W D PhillipsPubMed 31340406
- HRB open research 1:172018Chemical crosslinking analysis of β-dystroglycan in dystrophin-deficient skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra Murphy,Margit Zweyer,Rustam R Mundegar,Dieter Swandulla,Kay OhlendieckPubMed 35528858
- Cerebral cortex (New York, N.Y. : 1991) 28:4049-40622018KCC2 Regulates Dendritic Spine Formation in a Brain-Region Specific and BDNF Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatricia Nora Awad,Clara Akofa Amegandjin,Joanna Szczurkowska,Josianne Nuñes Carriço,Antônia Samia Fernandes do Nascimento,Elie Baho,Bidisha Chattopadhyaya,Laura Cancedda,Lionel Carmant,Graziella Di CristoPubMed 30169756
- Journal of proteomics 191:212-2272018Proteomic analysis of the sarcolemma-enriched fraction from dystrophic mdx-4cv skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra Murphy,Margit Zweyer,Michael Henry,Paula Meleady,Rustam R Mundegar,Dieter Swandulla,Kay OhlendieckPubMed 29408692
- BMC research notes 10:6012017Evaluation of the effect of a floxed Neo cassette within the dystroglycan (Dag1) gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesca Sciandra et. al.PubMed 29157305
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Dog | Predicted |
| Rabbit | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Dog | Dilution info - | Notes - |
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Target data
Function
The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Alpha-dystroglycan. Extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for laminin LAMA5 (By similarity). Beta-dystroglycan. Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity. Alpha-dystroglycan. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus glycoprotein and class C new-world arenaviruses (PubMed:16254364, PubMed:17360738, PubMed:19324387). Acts as a Schwann cell receptor for Mycobacterium leprae, the causative organism of leprosy, but only in the presence of the G-domain of LAMA2 (PubMed:9851927).
Alternative names
Dystroglycan 1, Dystroglycan, Dystrophin-associated glycoprotein 1
Recommended products
Rabbit Polyclonal DAG1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Dystroglycan also known as β-DG is a transmembrane protein with a molecular mass of approximately 43 kDa. It plays a mechanical role by linking the extracellular matrix to the cytoskeleton acting as a part of the dystrophin-glycoprotein complex. This protein is widely expressed in many tissues including skeletal muscle cardiac muscle and the central nervous system. Beta Dystroglycan serves as a receptor for components like laminin and agrin in the extracellular matrix.
- Biological function summary
Beta Dystroglycan acts as a structural and signaling molecule within the dystrophin-glycoprotein complex. This complex stabilizes the sarcolemma during muscle contraction and helps in protecting muscle fibers from injury. Beta Dystroglycan also contributes to cellular signaling pathways by interacting with various intracellular proteins like dystrophin and alpha dystroglycan. It regulates cell adhesion and migration processes critical for tissue maintenance and repair.
- Pathways
Beta Dystroglycan plays a critical role in the integrity of the dystrophin-associated glycoprotein complex. It is involved in the AKT signaling pathway which is essential for cellular growth and survival. Moreover beta Dystroglycan interacts closely with proteins such as dystrophin and utrophin facilitating their functions in muscle cells. Its interactions help maintain cell membrane stability and signal transduction processes important for normal cellular functions.
- Associated diseases and disorders
Beta Dystroglycan’s dysfunction has been closely linked to muscular dystrophies specifically Duchenne and Becker muscular dystrophies. These conditions involve muscle weakness and degeneration linked to abnormalities in the dystrophin-glycoprotein complex. Additionally aberrant glycosylation and signaling of beta Dystroglycan contribute to cancer progression through altered cell adhesion and migration often involving interactions with proteins like α-dystroglycan.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-beta Dystroglycan antibody (ab43125)
Lanes 1-3: Merged signal (red and green). Green - ab43125 observed at 43 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab43125 Anti-beta Dystroglycan antibody was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human DAG1 knockout HEK-293T cell line ab266263 (knockout cell lysate Human DAG1 knockout HEK-293T cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab43125 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-beta Dystroglycan antibody (ab43125) at 1/500 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human DAG1 knockout HEK-293T cell line (Human DAG1 knockout HEK-293T cell line ab266263)
Lane 2: DAG1 knockout HEK293T cell lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 43 kDa
Western blot - Anti-beta Dystroglycan antibody (ab43125)
Lanes 1-3: Merged signal (red and green). Green - ab43125 observed at 43 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab43125 Anti-beta Dystroglycan antibody was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human DAG1 knockout HEK-293T cell line ab266263 (knockout cell lysate Human DAG1 knockout HEK-293T cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab43125 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-beta Dystroglycan antibody (ab43125) at 1/500 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: DAG1 knockout HEK293T cell lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 43 kDa
Western blot - Anti-beta Dystroglycan antibody (ab43125)
All lanes: Western blot - Anti-beta Dystroglycan antibody (ab43125) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Human heart tissue lysate - total protein (ab29431) at 10 µg
Lane 3: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 43 kDa
Western blot - Anti-beta Dystroglycan antibody (ab43125)
ab43125 was shown to react with DAG1 in wild-type A431 cells in Western blot with loss of signal observed in a DAG1 knockout cell line. Wild-type A431 and DAG1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab43125 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Western blot - Anti-beta Dystroglycan antibody (ab43125) at 1/500 dilution
Lane 1: Wild-type A431 lysate at 90 µg
Lane 2: DAG1 knock-out A431 lysate at 90 µg
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