Anti-beta Dystroglycan antibody

• WB

Unknown

Western blot - Anti-beta Dystroglycan antibody (AB43125)

Lanes 1-3 : Merged signal (red and green). Green - ab43125 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab43125 Anti-beta Dystroglycan antibody was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266263 (knockout cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab43125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-beta Dystroglycan antibody (ab43125) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAG1 knockout HEK293T cell lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 43 kDa

false

• WB

Lab

Western blot - Anti-beta Dystroglycan antibody (AB43125)

Lanes 1-3 : Merged signal (red and green). Green - ab43125 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab43125 Anti-beta Dystroglycan antibody was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266263 (knockout cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab43125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-beta Dystroglycan antibody (ab43125) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human DAG1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-dag1-knockout-hek-293t-cell-line-ab266263'>ab266263</a>)

Lane 2:

DAG1 knockout HEK293T cell lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 43 kDa

false

• WB

Lab

Western blot - Anti-beta Dystroglycan antibody (AB43125)

ab43125 was shown to react with DAG1 in wild-type A431 cells in Western blot with loss of signal observed in a DAG1 knockout cell line. Wild-type A431 and DAG1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab43125 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-beta Dystroglycan antibody (ab43125) at 1/500 dilution

Lane 1:

Wild-type A431 lysate at 90 µg

Lane 2:

DAG1 knock-out A431 lysate at 90 µg

false

• WB

Project

Western blot - Anti-beta Dystroglycan antibody (AB43125)

All lanes:

Western blot - Anti-beta Dystroglycan antibody (ab43125) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 3:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 43 kDa

false