AB62373

Anti-beta Dystroglycan antibody [EP2200Y]

RabMAb
Recombinant
KO Validated
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(3 Publications)

Rabbit Recombinant Monoclonal DAG1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications.

View Alternative Names

Dystroglycan 1, Dystroglycan, Dystrophin-associated glycoprotein 1

5 Images

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Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 1/1000 dilution

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 97 kDa

Observed band size: 43 kDa

false

Lab

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

Lanes 1-3 : Merged signal (red and green). Green - ab62373 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab62373 Anti-beta Dystroglycan antibody [EP2200Y] was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266263 (knockout cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab62373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human DAG1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-dag1-knockout-hek-293t-cell-line-ab266263'>ab266263</a>)

Lane 2:

DAG1 knockout HEK293T cell lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 43 kDa

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Lab

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

ab62373 was shown to react with DAG1 in wild-type A431 cells in Western blot with loss of signal observed in DAG1 knockout cell line ab261906. Wild-type A431 and DAG1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab62373 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 1/1000 dilution

Lane 1:

Wild-type A431 lysate at 20 µg

Lane 2:

Western blot - Human DAG1 knockout A-431 cell line (ab261906) at 20 µg

Observed band size: 43 kDa

false

Lab

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

Blocking Buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 0.526 µg/mL

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Mouse heart lysate at 20 µg

Lane 3:

Mouse skeletal muscle at 20 µg

Lane 4:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 97 kDa

false

Unknown

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

Lanes 1-3 : Merged signal (red and green). Green - ab62373 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.

All lanes:

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAG1 knockout HEK293T cell lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 43 kDa

false

Carrier free

Anti-beta Dystroglycan antibody [EP2200Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2200Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p>Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p>Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Dystroglycan also known as β-DG is a transmembrane protein with a molecular mass of approximately 43 kDa. It plays a mechanical role by linking the extracellular matrix to the cytoskeleton acting as a part of the dystrophin-glycoprotein complex. This protein is widely expressed in many tissues including skeletal muscle cardiac muscle and the central nervous system. Beta Dystroglycan serves as a receptor for components like laminin and agrin in the extracellular matrix.

Biological function summary

Beta Dystroglycan acts as a structural and signaling molecule within the dystrophin-glycoprotein complex. This complex stabilizes the sarcolemma during muscle contraction and helps in protecting muscle fibers from injury. Beta Dystroglycan also contributes to cellular signaling pathways by interacting with various intracellular proteins like dystrophin and alpha dystroglycan. It regulates cell adhesion and migration processes critical for tissue maintenance and repair.

Pathways

Beta Dystroglycan plays a critical role in the integrity of the dystrophin-associated glycoprotein complex. It is involved in the AKT signaling pathway which is essential for cellular growth and survival. Moreover beta Dystroglycan interacts closely with proteins such as dystrophin and utrophin facilitating their functions in muscle cells. Its interactions help maintain cell membrane stability and signal transduction processes important for normal cellular functions.

Beta Dystroglycan’s dysfunction has been closely linked to muscular dystrophies specifically Duchenne and Becker muscular dystrophies. These conditions involve muscle weakness and degeneration linked to abnormalities in the dystrophin-glycoprotein complex. Additionally aberrant glycosylation and signaling of beta Dystroglycan contribute to cancer progression through altered cell adhesion and migration often involving interactions with proteins like α-dystroglycan.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.. Alpha-dystroglycan. Extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for laminin LAMA5 (By similarity).. Beta-dystroglycan. Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity.. Alpha-dystroglycan. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus glycoprotein and class C new-world arenaviruses (PubMed : 16254364, PubMed : 17360738, PubMed : 19324387). Acts as a Schwann cell receptor for Mycobacterium leprae, the causative organism of leprosy, but only in the presence of the G-domain of LAMA2 (PubMed : 9851927).

See full target information Dystroglycan

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10541 PubMed39627191

2024

Cell polarity proteins promote macropinocytosis in response to metabolic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Guillem Lambies,Szu-Wei Lee,Karen Duong-Polk,Pedro Aza-Blanc,Swetha Maganti,Cheska M Galapate,Anagha Deshpande,Aniruddha J Deshpande,David A Scott,David W Dawson,Cosimo Commisso

Human molecular genetics 33:709-723 PubMed38272461

2024

Removal of pomt1 in zebrafish leads to loss of α-dystroglycan glycosylation and dystroglycanopathy phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany F Karas,Kristin R Terez,Shorbon Mowla,Namarata Battula,Kyle P Flannery,Brian M Gural,Grace Aboussleman,Numa Mubin,M Chiara Manzini

Foods (Basel, Switzerland) 11: PubMed35954013

2022

Shrinkage Properties and Their Relationship with Degradation of Proteins Linking the Endomysium and Myofibril in Lamb Meat Submitted to Heating or Air Drying.

Applications

Unspecified application

Species

Unspecified reactive species

Weili Rao,Zhenxiao Shi,Sijia Liu,Ying Shu,Xiaoyu Chai,Zhisheng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Dystroglycan antibody [EP2200Y]

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

Western blot - Anti-beta Dystroglycan antibody [EP2200Y] (AB62373)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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