AB134435

Anti-beta Galactosidase antibody

Name: Anti-beta Galactosidase antibody (ab134435) | Abcam
Brand: Abcam Limited
SKU: AB134435
Price: 525 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(12 Publications)

Chicken Polyclonal GLB1/Beta-galactosidase antibody. Suitable for IHC-FoFr, ICC/IF and reacts with Escherichia coli samples. Cited in 12 publications.

View Alternative Names

b0344, JW0335, lacZ, Beta-galactosidase, Beta-gal, Lactase

1 Images

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-beta Galactosidase antibody (AB134435)

IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-beta Galactosidase antibody (AB134435)

Immunohistochemical analysis of tissue infected with a virus bearing the LacZ gene labelling beta Galactosidase with ab134435 at 1/5000 dilution with e18 mouse otic vesicle. The green stain is beta-galactosidase (ab134435); the red stain is Texas-red-phalloidin, staining filamentous actin; the blue staining is DAPI (4',6-diamidino-2-phenylindole), a DNA-binding dye.Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1/2000 dilution).

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Escherichia coli

Applications

IHC-FoFr, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "1/2500 - 1/5000", "IHCFoFr-species-notes": "<p>Use 2% paraformaldehyde-fixed tissues infected with a virus bearing the LacZ gene.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/2500 - 1/5000", "ICCIF-species-notes": "<p>Use 2%paraformaldehyde-fixed cells.</p>" } } }

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Product details

Do not freeze these antibodies at –80°C unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

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Properties and storage information

Form

Liquid

Purity

IgY fraction

Purification notes

IgY fractions were prepared from eggs yolks, and then affinity-purified antibodies were prepared using beta Galactosidase conjugatedto an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/ml) and purified IgY (10 mg/ml), and is filter-sterilized.

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Galactosidase often called "β-gal" "b-galactosidase" or "b-gal" serves as an important enzyme that hydrolyzes β-galactosides into monosaccharides. It breaks down lactose into glucose and galactose which are easier to absorb. Beta-galactosidase expresses in many organisms such as *E. coli* where it is a well-known component of the lac operon. Its molecular weight in *E. coli* is approximately 116 kDa. The enzyme also appears in human tissues but is synthesized and regulated differently compared to bacterial systems.

Biological function summary

This enzyme is involved in the metabolism of galactose a critical aspect of cellular energy production. It can participate in various metabolic pathways converting complex carbohydrates into simpler sugars. In bacteria beta-galactosidase belongs to a protein complex controlled by the lac operon regulating lactose metabolism. In humans the enzyme takes part in lysosomal functions contributing to the degradation of glycoproteins and glycolipids therefore supporting normal cellular processes.

Pathways

Beta-galactosidase plays an important role in the lactose metabolism pathway. In *E. coli* it coheres with the enzymes of the lac system like lactose permease and transacetylase to facilitate lactose utilization. In human biology it participates in the lysosomal degradation pathway. Its function is associated with proteins such as galactosylceramidase which also break down galactolipids demonstrating its interaction in broader metabolic networks.

Deficiencies or malfunctions in beta-galactosidase lead to metabolic conditions like Galactosialidosis and Morquio syndrome Type B. These are lysosomal storage disorders caused by a lack of enzyme activity leading to accumulation of partially degraded macromolecules. The enzyme's impaired function connects with other proteins such as protective protein cathepsin A in the case of Galactosialidosis causing complex clinical symptoms through disrupted protein interactions and pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information lacZ

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 8:455 PubMed36371343

2022

MiMIC analysis reveals an isoform specific role for Drosophila Musashi in follicle stem cell maintenance and escort cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole A Siddall,Franca Casagranda,Timothy M Johanson,Nicole Dominado,James Heaney,Jessie M Sutherland,Eileen A McLaughlin,Gary R Hime

American journal of physiology. Renal physiology 323:F272-F287 PubMed35862649

2022

Essential role of Wtip in mouse development and maintenance of the glomerular filtration barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Sethu M Madhavan,Martha Konieczkowski,Leslie A Bruggeman,Megan DeWalt,Jane K Nguyen,John F O'Toole,John R Sedor

Cell reports 33:108305 PubMed33113378

2020

DWnt4 and DWnt10 Regulate Morphogenesis and Arrangement of Columnar Units via Fz2/PCP Signaling in the Drosophila Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Xujun Han,Miaoxing Wang,Chuyan Liu,Olena Trush,Rie Takayama,Takaaki Akiyama,Toshiki Naito,Takeshi Tomomizu,Kousuke Imamura,Makoto Sato

eLife 9: PubMed33112231

2020

Coordinated crosstalk between microtubules and actin by a spectraplakin regulates lumen formation and branching.

Applications

Unspecified application

Species

Unspecified reactive species

Delia Ricolo,Sofia J Araujo

The EMBO journal 39:e101767 PubMed33021744

2020

Glycolysis regulates Hedgehog signalling via the plasma membrane potential.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Spannl,Tomasz Buhl,Ioannis Nellas,Salma A Zeidan,K Venkatesan Iyer,Helena Khaliullina,Carsten Schultz,André Nadler,Natalie A Dye,Suzanne Eaton

JCI insight 5: PubMed32229724

2020

MT1-MMP deficiency leads to defective ependymal cell maturation, impaired ciliogenesis, and hydrocephalus.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Jiang,Jin Zhou,Xin Qin,Huiling Zheng,Bo Gao,Xinguang Liu,Guoxiang Jin,Zhongjun Zhou

Scientific reports 10:517 PubMed31949274

2020

POU domain motif3 (Pdm3) induces wingless (wg) transcription and is essential for development of larval neuromuscular junctions in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon Kim,Kyung-Ok Cho

The Journal of biological chemistry 294:11498-11512 PubMed31177092

2019

Neurolastin, a dynamin family GTPase, translocates to mitochondria upon neuronal stress and alters mitochondrial morphology .

Applications

Unspecified application

Species

Unspecified reactive species

Richa Madan Lomash,Ronald S Petralia,Lynne A Holtzclaw,Mumeko C Tsuda,Ya-Xian Wang,John D Badger,Heather A Cameron,Richard J Youle,Katherine W Roche

Glia 67:1667-1679 PubMed31050055

2019

Vaccinia-related kinase 2 plays a critical role in microglia-mediated synapse elimination during neurodevelopment.

Applications

Unspecified application

Species

Unspecified reactive species

Juhyun Lee,Seunghyun Lee,Young-Jae Ryu,Dohyun Lee,Sangjune Kim,Ji-Young Seo,Eunji Oh,Sun Ha Paek,Seung U Kim,Chang-Man Ha,Se-Young Choi,Kyong-Tai Kim

Development (Cambridge, England) 145: PubMed29980566

2018

Eyeless/Pax6 initiates eye formation non-autonomously from the peripodial epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Luke R Baker,Bonnie M Weasner,Athena Nagel,Sarah D Neuman,Arash Bashirullah,Justin P Kumar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta Galactosidase antibody

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-beta Galactosidase antibody (AB134435)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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