AB221199

Anti-beta Galactosidase antibody

Name: Anti-beta Galactosidase antibody (ab221199) | Abcam
Brand: Abcam Limited
SKU: AB221199
Price: 415 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GLB1/Beta-galactosidase antibody. Suitable for ICC/IF, IP, WB and reacts with Transfected cell line - Escherichia coli, Escherichia coli, Recombinant full length protein - Escherichia coli samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Escherichia coli O127:H6 str. E2348/69 lacZ.

View Alternative Names

E2348C_0299, lacZ, Beta-galactosidase, Beta-gal, Lactase, Beta D galactosidase, Beta gal, ECK0341, JW0335, Lactase, lacZ

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (AB221199)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (AB221199)

Immunofluorescent analysis of HEK293A cells transfected with beta Galactosidase cDNA (4% PFA-fixed, methanol permeabilized) labeling beta Galactosidase with ab221199 at 1/500 dilution, followed by secondary detection with Alexa Fluor 555 conjugated anti rabbit IgG at 1/500 dilution (red). Hoecst 33342 was used for nuclear counterstaining (blue).

Supplier Data

Immunoprecipitation - Anti-beta Galactosidase antibody (AB221199)

beta Galactosidase was immunorecipitated from ITPG induced E coli crude extracts using ab221199 at 1/500 dilution.

Western blot detection was performed using ab221199 at 1/1000 dilution.

Lane 1 : Crude extract

Lane 2 : Supernatant of the immunoprecipitated E. coli crude extract

Lane 3 : Immunoprecipitate of crude E. coli extract

All lanes:

Immunoprecipitation - Anti-beta Galactosidase antibody (ab221199)

Predicted band size: 116 kDa

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Supplier Data

Western blot - Anti-beta Galactosidase antibody (AB221199)

All lanes:

Western blot - Anti-beta Galactosidase antibody (ab221199) at 1/1000 dilution

Lane 1:

purified beta Galactosidase at 0.01 µg

Lane 2:

uninduced E coli cell extract at 30 µg

Lane 3:

ITPG induced E coli cell extract at 30 µg

Predicted band size: 116 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Escherichia coli

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Escherichia coli O127:H6 str. E2348/69 lacZ.

B7UJI9

Specificity

This antibody recognizes beta Galactosidase from E coli and beta Galactosidase tagged proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" }, "Recombinant full length protein - Escherichia coli": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" }, "Transfected cell line - Escherichia coli": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Galactosidase often called "β-gal" "b-galactosidase" or "b-gal" serves as an important enzyme that hydrolyzes β-galactosides into monosaccharides. It breaks down lactose into glucose and galactose which are easier to absorb. Beta-galactosidase expresses in many organisms such as E. coli where it is a well-known component of the lac operon. Its molecular weight in E. coli is approximately 116 kDa. The enzyme also appears in human tissues but is synthesized and regulated differently compared to bacterial systems.

Biological function summary

This enzyme is involved in the metabolism of galactose a critical aspect of cellular energy production. It can participate in various metabolic pathways converting complex carbohydrates into simpler sugars. In bacteria beta-galactosidase belongs to a protein complex controlled by the lac operon regulating lactose metabolism. In humans the enzyme takes part in lysosomal functions contributing to the degradation of glycoproteins and glycolipids therefore supporting normal cellular processes.

Pathways

Beta-galactosidase plays an important role in the lactose metabolism pathway. In E. coli it coheres with the enzymes of the lac system like lactose permease and transacetylase to facilitate lactose utilization. In human biology it participates in the lysosomal degradation pathway. Its function is associated with proteins such as galactosylceramidase which also break down galactolipids demonstrating its interaction in broader metabolic networks.

Deficiencies or malfunctions in beta-galactosidase lead to metabolic conditions like Galactosialidosis and Morquio syndrome Type B. These are lysosomal storage disorders caused by a lack of enzyme activity leading to accumulation of partially degraded macromolecules. The enzyme's impaired function connects with other proteins such as protective protein cathepsin A in the case of Galactosialidosis causing complex clinical symptoms through disrupted protein interactions and pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information lacZ

Additional targets

beta Galactosidase

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40737093

2025

S-phase checkpoint protects from aberrant replication fork processing and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Iván Núñez-Martín,Lucy S Drury,María I Martínez-Jiménez,Luis Blanco,John F X Diffley,Andrés Aguilera,Belén Gómez-González

Cell reports 39:110663 PubMed35417692

2022

A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.

Applications

Unspecified application

Species

Unspecified reactive species

Olivier Mauduit,Marit H Aure,Vanessa Delcroix,Liana Basova,Amrita Srivastava,Takeshi Umazume,Jacqueline W Mays,Saverio Bellusci,Abigail S Tucker,Mohammad K Hajihosseini,Matthew P Hoffman,Helen P Makarenkova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Galactosidase antibody

Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (AB221199)

Immunoprecipitation - Anti-beta Galactosidase antibody (AB221199)

Western blot - Anti-beta Galactosidase antibody (AB221199)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Product promise