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AB616

Anti-beta Galactosidase antibody

4

(30 Reviews)

|

(118 Publications)

Rabbit Polyclonal GLB1/Beta-galactosidase antibody. Suitable for WB and reacts with Escherichia coli samples. Cited in 118 publications.

View Alternative Names

b0344, JW0335, lacZ, Beta-galactosidase, Beta-gal, Lactase

1 Images
Western blot - Anti-beta Galactosidase antibody (AB616)
  • WB

Supplier Data

Western blot - Anti-beta Galactosidase antibody (AB616)

Western blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.

All lanes:

Western blot - Anti-beta Galactosidase antibody (ab616)

Lanes 1 - 2:

Non-reduced purified recombinant beta Galactosidase protein

Lanes 3 - 4:

reduced purified recombinant beta Galactosidase protein

Predicted band size: 116 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Escherichia coli

Applications

WB

applications

Specificity

Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.

Reactivity data

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Product details

Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.

Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification technique
Ion exchange chromatography
Purification notes
Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Galactosidase often called "β-gal" "b-galactosidase" or "b-gal" serves as an important enzyme that hydrolyzes β-galactosides into monosaccharides. It breaks down lactose into glucose and galactose which are easier to absorb. Beta-galactosidase expresses in many organisms such as *E. coli* where it is a well-known component of the lac operon. Its molecular weight in *E. coli* is approximately 116 kDa. The enzyme also appears in human tissues but is synthesized and regulated differently compared to bacterial systems.
Biological function summary

This enzyme is involved in the metabolism of galactose a critical aspect of cellular energy production. It can participate in various metabolic pathways converting complex carbohydrates into simpler sugars. In bacteria beta-galactosidase belongs to a protein complex controlled by the lac operon regulating lactose metabolism. In humans the enzyme takes part in lysosomal functions contributing to the degradation of glycoproteins and glycolipids therefore supporting normal cellular processes.

Pathways

Beta-galactosidase plays an important role in the lactose metabolism pathway. In *E. coli* it coheres with the enzymes of the lac system like lactose permease and transacetylase to facilitate lactose utilization. In human biology it participates in the lysosomal degradation pathway. Its function is associated with proteins such as galactosylceramidase which also break down galactolipids demonstrating its interaction in broader metabolic networks.

Deficiencies or malfunctions in beta-galactosidase lead to metabolic conditions like Galactosialidosis and Morquio syndrome Type B. These are lysosomal storage disorders caused by a lack of enzyme activity leading to accumulation of partially degraded macromolecules. The enzyme's impaired function connects with other proteins such as protective protein cathepsin A in the case of Galactosialidosis causing complex clinical symptoms through disrupted protein interactions and pathways.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (118)

Recent publications for all applications. Explore the full list and refine your search

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:427-442 PubMed36625422

2023

Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH) D Deficiency and Downregulates GATA4-Dependent Rela Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyi Wang,Jingyu Zhao,Haiyun Chen,Jiawen Zhou,Ao Chen,Jin'ge Zhang,Yue Wang,Zhiyuan Mao,Jiachen Wang,Xuehan Qiu,Yutong Chen,Rong Wang,Yongjie Zhang,Dengshun Miao,Jianliang Jin

International journal of biological sciences 19:610-624 PubMed36632467

2023

1,25-Dihydroxyvitamin D Deficiency Accelerates Aging-related Osteoarthritis via Downregulation of Sirt1 in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Jiao Zhang,Jie Li,Ran Qin,Na Lu,David Goltzman,Dengshun Miao,Renlei Yang

Disease models & mechanisms 15: PubMed35972154

2022

Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survival.

Applications

Unspecified application

Species

Unspecified reactive species

Kathrin Pfeifer,Georg Wolfstetter,Vimala Anthonydhason,Tafheem Masudi,Badrul Arefin,Mats Bemark,Patricia Mendoza-Garcia,Ruth H Palmer

Scientific reports 11:11968 PubMed34099837

2021

Osteogenic differentiation of human mesenchymal stromal cells and fibroblasts differs depending on tissue origin and replicative senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Grotheer,Nadine Skrynecki,Lisa Oezel,Joachim Windolf,Jan Grassmann

European cells & materials 41:194-203 PubMed33620083

2021

Spatiotemporal and functional characterisation of transient receptor potential vanilloid 4 (TRPV4) in the murine intervertebral disc.

Applications

Unspecified application

Species

Unspecified reactive species

M Km Kim,R Ramachandran,C A Séguin

Gastroenterology 159:1487-1503.e17 PubMed32574624

2020

IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Debanjali Dasgupta,Yasuhiko Nakao,Amy S Mauer,Jill M Thompson,Tejasav S Sehrawat,Chieh-Yu Liao,Anuradha Krishnan,Fabrice Lucien,Qianqian Guo,Mengfei Liu,Fei Xue,Masanori Fukushima,Tomohiro Katsumi,Aditya Bansal,Mukesh K Pandey,Jessica L Maiers,Timothy DeGrado,Samar H Ibrahim,Alexander Revzin,Kevin D Pavelko,Michael A Barry,Randal J Kaufman,Harmeet Malhi

Nature communications 11:1990 PubMed32332749

2020

Identification of therapeutics that target eEF1A2 and upregulate utrophin A translation in dystrophic muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Christine Péladeau,Nadine Adam,Lucas M Bronicki,Adèle Coriati,Mohamed Thabet,Hasanen Al-Rewashdy,Jason Vanstone,Alan Mears,Jean-Marc Renaud,Martin Holcik,Bernard J Jasmin

Cells 9: PubMed32164318

2020

Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Hilbold,Ottmar Distl,Martina Hoedemaker,Sandra Wilkening,Rüdiger Behr,Aleksandar Rajkovic,Marion Langeheine,Kristina Rode,Klaus Jung,Julia Metzger,Ralph H J Brehm

American journal of translational research 12:507-518 PubMed32194899

2020

1,25-Dihydroxyvitamin D insufficiency accelerates age-related bone loss by increasing oxidative stress and cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Wanxin Qiao,Shuxiang Yu,Haijian Sun,Lulu Chen,Rong Wang,Xuan Wu,David Goltzman,Dengshun Miao

Nature communications 9:5293 PubMed30546048

2018

Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Heuijoon Park,Sonali Lad,Kelsey Boland,Kelly Johnson,Nyssa Readio,Guangchun Jin,Samuel Asfaha,Kelly S Patterson,Ashok Singh,Xiangdong Yang,Douglas Londono,Anupama Singh,Carol Trempus,Derek Gordon,Timothy C Wang,Rebecca J Morris
View all publications

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