Rabbit Polyclonal GLB1/Beta-galactosidase antibody. Suitable for WB and reacts with Escherichia coli samples. Cited in 118 publications.
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- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:427-4422023Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH) D Deficiency and Downregulates GATA4-Dependent Rela Transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuyi Wang,Jingyu Zhao,Haiyun Chen,Jiawen Zhou,Ao Chen,Jin'ge Zhang,Yue Wang,Zhiyuan Mao,Jiachen Wang,Xuehan Qiu,Yutong Chen,Rong Wang,Yongjie Zhang,Dengshun Miao,Jianliang JinPubMed 36625422
- International journal of biological sciences 19:610-62420231,25-Dihydroxyvitamin D Deficiency Accelerates Aging-related Osteoarthritis via Downregulation of Sirt1 in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Chen,Jiao Zhang,Jie Li,Ran Qin,Na Lu,David Goltzman,Dengshun Miao,Renlei YangPubMed 36632467
- Disease models & mechanisms 15:2022Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathrin Pfeifer,Georg Wolfstetter,Vimala Anthonydhason,Tafheem Masudi,Badrul Arefin,Mats Bemark,Patricia Mendoza-Garcia,Ruth H PalmerPubMed 35972154
- Scientific reports 11:119682021Osteogenic differentiation of human mesenchymal stromal cells and fibroblasts differs depending on tissue origin and replicative senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVera Grotheer,Nadine Skrynecki,Lisa Oezel,Joachim Windolf,Jan GrassmannPubMed 34099837
- European cells & materials 41:194-2032021Spatiotemporal and functional characterisation of transient receptor potential vanilloid 4 (TRPV4) in the murine intervertebral disc.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Km Kim,R Ramachandran,C A SéguinPubMed 33620083
- Gastroenterology 159:1487-1503.e172020IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDebanjali Dasgupta et. al.PubMed 32574624
- Nature communications 11:19902020Identification of therapeutics that target eEF1A2 and upregulate utrophin A translation in dystrophic muscles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristine Péladeau et. al.PubMed 32332749
- Cells 9:2020Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErika Hilbold et. al.PubMed 32164318
- American journal of translational research 12:507-51820201,25-Dihydroxyvitamin D insufficiency accelerates age-related bone loss by increasing oxidative stress and cell senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanxin Qiao et. al.PubMed 32194899
- Nature communications 9:52932018Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeuijoon Park et. al.PubMed 30546048
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Lyophilized
- Clonality
- Polyclonal
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | ICC/IF | |
|---|---|---|---|---|
| Escherichia coli | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli | Dilution info 1/5000 - 1/10000 | Notes This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend Anti-beta Galactosidase antibody ab4761 or Anti-beta Galactosidase antibody ab12081 as alternatives. |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Alternative names
b0344, JW0335, lacZ, Beta-galactosidase, Beta-gal, Lactase
Recommended products
Rabbit Polyclonal GLB1/Beta-galactosidase antibody. Suitable for WB and reacts with Escherichia coli samples. Cited in 118 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Lyophilized
- Clonality
- Polyclonal
- Purification technique
- Ion exchange chromatography
- Specificity
Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
- Concentration
- Purification notes
Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend Anti-beta Galactosidase antibody ab4761 or Anti-beta Galactosidase antibody ab12081 as alternatives.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Galactosidase often called "β-gal" "b-galactosidase" or "b-gal" serves as an important enzyme that hydrolyzes β-galactosides into monosaccharides. It breaks down lactose into glucose and galactose which are easier to absorb. Beta-galactosidase expresses in many organisms such as *E. coli* where it is a well-known component of the lac operon. Its molecular weight in *E. coli* is approximately 116 kDa. The enzyme also appears in human tissues but is synthesized and regulated differently compared to bacterial systems.
- Biological function summary
This enzyme is involved in the metabolism of galactose a critical aspect of cellular energy production. It can participate in various metabolic pathways converting complex carbohydrates into simpler sugars. In bacteria beta-galactosidase belongs to a protein complex controlled by the lac operon regulating lactose metabolism. In humans the enzyme takes part in lysosomal functions contributing to the degradation of glycoproteins and glycolipids therefore supporting normal cellular processes.
- Pathways
Beta-galactosidase plays an important role in the lactose metabolism pathway. In *E. coli* it coheres with the enzymes of the lac system like lactose permease and transacetylase to facilitate lactose utilization. In human biology it participates in the lysosomal degradation pathway. Its function is associated with proteins such as galactosylceramidase which also break down galactolipids demonstrating its interaction in broader metabolic networks.
- Associated diseases and disorders
Deficiencies or malfunctions in beta-galactosidase lead to metabolic conditions like Galactosialidosis and Morquio syndrome Type B. These are lysosomal storage disorders caused by a lack of enzyme activity leading to accumulation of partially degraded macromolecules. The enzyme's impaired function connects with other proteins such as protective protein cathepsin A in the case of Galactosialidosis causing complex clinical symptoms through disrupted protein interactions and pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-beta Galactosidase antibody (ab616)
Western blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.
All lanes: Western blot - Anti-beta Galactosidase antibody (ab616)
Lanes 1 - 2: Non-reduced purified recombinant beta Galactosidase protein
Lanes 3 - 4: reduced purified recombinant beta Galactosidase protein
Predicted band size: 116 kDa
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