Anti-beta Galactosidase antibody

• IHC-FoFr

PubMed

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-beta Galactosidase antibody (AB9361)

P0-adult mice were euthanized and perfused with 4% paraformaldehyde in PBS (PF). Their spinal cords were then post-fixed for 30–60 mins in 4% PF at 4°C (P0) or at room temperature (adult). Spinal cords were rinsed and cryoprotected in 20% sucrose in PBS (4°C) prior to embedding in OCT (Tissue-Tek). Immunostaining of frozen spinal sections was performed by incubating 20 μm thick sections with primary antibodies, which were then detected using species-specific secondary antibodies conjugated with Cy2, Cy3 and Cy5 or FITC. ab9361 was used at 1 : 1000.

Image from PLoS One. 2013; 8(11): e77928, Fig 2, doi: 10.1371/journal.pone.0077928 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

AbReview7239****

Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (AB9361)

ab9361 at 1/250 staining human HEK293T cells by ICC/IF. The cell line was transfected with a b-gal expressing plasmid, and x-gal staining was performed on adjacent wells. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. A Texas Red conjugated donkey anti-chicken antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Deon Wolpowitz

• IHC-Fr

AbReview17899****

Immunohistochemistry (Frozen sections) - Anti-beta Galactosidase antibody (AB9361)

ab9361 staining Beta Galactosidase in mouse retinal tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.2% triton-X and blocked with 5% serum for 1 hour at 23°C. The sample was incubated with primary antibody (1/1500 in PBS, 2% serum, 0.2% Triton-X) at 4°C for 16 hours. An Alexa Fluor^® 488-conjugated Goat monoclonal to chicken IgY (1/200) was used as secondary antibody.

This image is courtesy of an Abreview submitted by Dr Steven Hughes

• IHC-P

AbReview41855****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (AB9361)

ab9361 staining beta Galactosidase in mouse e13 stomach and liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Davidson's fixative, permeabilized with 0.5% Triton-X 100 and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBST + 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Laurel Baglia

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (AB9361)

ab9361 staining Beta galactosidase in Mouse thyroid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed 10% buffered formalin, cut into 3-4 micron slices, blocked with 10% normal goat serum and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with the following primary antibodies; anti-BrdU, anti-Sca1 (ab109211, 1∶600), anti-NKX2-1 and anti-β-gal (ab9361, 1∶1000). Sections were incubated with the first primary antibody (anti-Sca1) for 1 hour at room temperature. After washing with PBS, sections were incubated with the first secondary antibody (Alexa Fluor 555 goat anti-rabbit IgG) and washed with PBS. Sections were then incubated with normal serum (5% rabbit serum) from the same host species as the first primary antibodies for 1 hour at room temperature and washed with PBS. Sections were further incubated with an excess of unconjugated Fab antibody derived from the same host species as the primary antibody for 1 hour at room temperature and washed with PBS. The sections were finally incubated with the mixed second primary antibodies (anti-BrdU, anti-β-gal, anti-NKX2-1) overnight at 4°C, washed with PBS, and were incubated with the second secondary antibody (Alexa Fluor 488 goat anti-rat IgG, Dylight 650 goat anti-chicken IgY, Dylight 594 goat anti-rabbit IgG) for 1 hour at room temperature and washed with PBS. DAPI dye was used to stain the nuclei of cells.

Image from PLoS One. 2013; 8(11): e80801, Fig 1B, doi: 10.1371/journal.pone.0080801 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

• ICC/IF

AbReview23333****

Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (AB9361)

ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4°C. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green).

Image courtesy of Dr Sean Speese by Abreview.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (AB9361)

Immunofluorescent analysis of 4% paraformaldehyde (PFA) -fixed, permeabilized with 0.1% Triton X-100 in CHO cells transfected with Beta-Galactosidase constructs (named as CHO-BGal) labelling Beta Galactosidase with ab9361 at 1/1000 dilution (1µg/mL), followed by Donkey Anti-chicken IgY (H&L), Alexa Fluor594 conjugated antibody at 1/1000 dilution (1 µg/mL) (Red). Nucleus was counterstained with DAPI (Blue). Images also showed Parallel staining in parental CHO cell line.