AB116

Anti-beta Galactosidase antibody [DC1 4C7]

Name: Anti-beta Galactosidase antibody [DC1 4C7] (ab116) | Abcam
Brand: Abcam Limited
SKU: AB116
Price: 475 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal GLB1/Beta-galactosidase antibody. Suitable for WB, ELISA and reacts with Escherichia coli samples. Cited in 8 publications.

View Alternative Names

b0344, JW0335, lacZ, Beta-galactosidase, Beta-gal, Lactase, Beta D galactosidase, Beta gal, ECK0341, JW0335, Lactase, lacZ

1 Images

Lab

Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (AB116)

All lanes:

Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (ab116) at 1 µg

Lane 1:

Western blot - Recombinant Human GLB1/Beta-galactosidase protein (<a href='/en-us/products/proteins-peptides/recombinant-human-glb1-beta-galactosidase-protein-ab151890'>ab151890</a>) at 0.1 µg

Lane 2:

Western blot - Native E. coli beta Galactosidase protein (<a href='/en-us/products/proteins-peptides/native-e-coli-beta-galactosidase-protein-ab79449'>ab79449</a>) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

Predicted band size: 116 kDa,118 kDa,183 kDa,43 kDa,54 kDa,56 kDa,72 kDa,74 kDa,84 kDa

false

Exposure time: 4min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DC1 4C7

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Escherichia coli

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }

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Product details

This monoclonal is also known as clone HD4C7 or HD 447C.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Galactosidase often called "β-gal" "b-galactosidase" or "b-gal" serves as an important enzyme that hydrolyzes β-galactosides into monosaccharides. It breaks down lactose into glucose and galactose which are easier to absorb. Beta-galactosidase expresses in many organisms such as E. coli where it is a well-known component of the lac operon. Its molecular weight in E. coli is approximately 116 kDa. The enzyme also appears in human tissues but is synthesized and regulated differently compared to bacterial systems.

Biological function summary

This enzyme is involved in the metabolism of galactose a critical aspect of cellular energy production. It can participate in various metabolic pathways converting complex carbohydrates into simpler sugars. In bacteria beta-galactosidase belongs to a protein complex controlled by the lac operon regulating lactose metabolism. In humans the enzyme takes part in lysosomal functions contributing to the degradation of glycoproteins and glycolipids therefore supporting normal cellular processes.

Pathways

Beta-galactosidase plays an important role in the lactose metabolism pathway. In E. coli it coheres with the enzymes of the lac system like lactose permease and transacetylase to facilitate lactose utilization. In human biology it participates in the lysosomal degradation pathway. Its function is associated with proteins such as galactosylceramidase which also break down galactolipids demonstrating its interaction in broader metabolic networks.

Deficiencies or malfunctions in beta-galactosidase lead to metabolic conditions like Galactosialidosis and Morquio syndrome Type B. These are lysosomal storage disorders caused by a lack of enzyme activity leading to accumulation of partially degraded macromolecules. The enzyme's impaired function connects with other proteins such as protective protein cathepsin A in the case of Galactosialidosis causing complex clinical symptoms through disrupted protein interactions and pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information lacZ

Additional targets

beta Galactosidase

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:813 PubMed38071330

2023

Lysosomes, caspase-mediated apoptosis, and cytoplasmic activation of P21, but not cell senescence, participate in a redundant fashion in embryonic morphogenetic cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Duarte-Olivenza,Goretti Moran,Juan M Hurle,Carlos I Lorda-Diez,Juan A Montero

Nature communications 12:6941 PubMed34862367

2021

Inhibition of cytoplasmic EZH2 induces antitumor activity through stabilization of the DLC1 tumor suppressor protein.

Applications

Unspecified application

Species

Unspecified reactive species

Brajendra K Tripathi,Meghan F Anderman,Disha Bhargava,Luciarita Boccuzzi,Xiaolan Qian,Dunrui Wang,Marian E Durkin,Alex G Papageorge,Fernando J de Miguel,Katerina Politi,Kylie J Walters,James H Doroshow,Douglas R Lowy

Nature 523:329-32 PubMed26131929

2015

Nanotubes mediate niche-stem-cell signalling in the Drosophila testis.

Applications

Unspecified application

Species

Unspecified reactive species

Mayu Inaba,Michael Buszczak,Yukiko M Yamashita

PloS one 7:e52335 PubMed23272238

2012

Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition.

Applications

IHC-P

Species

Unspecified reactive species

Stuart J Smith,Martin Wilson,Jennifer H Ward,Cheryl V Rahman,Andrew C Peet,Donald C Macarthur,Felicity R A J Rose,Richard G Grundy,Ruman Rahman

Arteriosclerosis, thrombosis, and vascular biology 30:1423-9 PubMed20431063

2010

Repressors NFI and NFY participate in organ-specific regulation of von Willebrand factor promoter activity in transgenic mice.

Applications

IHC-Fr

Species

Unspecified reactive species

Marjan Nassiri,Ju Liu,Stephen Kulak,Richard R E Uwiera,William C Aird,Barbara J Ballermann,Nadia Jahroudi

Developmental biology 330:329-38 PubMed19361496

2009

Multiple lineage-specific roles of Smad4 during neural crest development.

Applications

Unspecified application

Species

Unspecified reactive species

Stine Büchmann-Møller,Iris Miescher,Nessy John,Jaya Krishnan,Chu-Xia Deng,Lukas Sommer

The Journal of biological chemistry 283:2741-50 PubMed18048367

2007

Sequences in intron 51 of the von Willebrand factor gene target promoter activation to a subset of lung endothelial cells in transgenic mice.

Applications

IHC-Fr

Species

Unspecified reactive species

Ann M Kleinschmidt,Marjan Nassiri,Molly S Stitt,Karla Wasserloos,Simon C Watkins,Bruce R Pitt,Nadia Jahroudi

Genesis (New York, N.Y. : 2000) 41:73-80 PubMed15682388

2005

Rhodopsin-iCre transgenic mouse line for Cre-mediated rod-specific gene targeting.

Applications

IHC

Species

Unspecified reactive species

Sha Li,Desheng Chen,Yves Sauvé,Jeremy McCandless,Yu-Jiun Chen,Ching-Kang Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Galactosidase antibody [DC1 4C7]

Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (AB116)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (8)

Product promise