AB50148

Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end

Name: Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end (ab50148) | Abcam
Brand: Abcam Limited
SKU: AB50148

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(11 Publications)

Rabbit Polyclonal beta glucuronidase (GUSB) antibody. Suitable for WB and reacts with Escherichia coli samples. Cited in 11 publications.

View Alternative Names

gurA, gusA, b1617, JW1609, uidA, Beta-glucuronidase, GUS, Beta-D-glucuronoside glucuronosohydrolase, EcGUS, UID

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Escherichia coli

Applications

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Staining of the beta Glucuronidase band in immunoblotting is specifically inhibited by the immunizing peptide.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta Glucuronidase (GUS) sometimes referred to as E. coli Beta-Glucuronidase or GUS Beta is an enzyme involved in hydrolyzing the beta-D-glucuronic acid residues from the non-reducing end of glycosaminoglycans. This enzymatic function makes it vital for the breakdown and recycling of complex carbohydrates in cells. GUS has a molecular mass of approximately 290 kDa and is expressed in various tissues with high levels observed in the liver and kidneys which are integral components of excretory processes.

Biological function summary

Beta Glucuronidase plays an important role in the lysosomal degradation of glycosaminoglycans. This process helps remove waste substances from the body effectively and deficiency in this enzyme may lead to the accumulation of undegraded molecules. Not part of a large complex GUS functions independently as a single protein catalytic unit. Researchers often employ the GUS assay to monitor its activity in analytical and clinical contexts indicating its importance in cellular metabolism research.

Pathways

Beta Glucuronidase is integral to the glycosaminoglycan degradation pathway which is essential for catabolizing heparan sulfate and dermatan sulfate. This pathway is significant for cellular turnover and tissue remodeling. Related proteins within this metabolic framework include other lysosomal hydrolases such as acid alpha-glucosidase which work concertedly to maintain cellular homeostasis by degrading complex carbohydrates.

Deficient activity of Beta Glucuronidase links to mucopolysaccharidosis (MPS) specifically MPS VII also known as Sly syndrome. This genetic condition results in various systemic complications including skeletal and developmental abnormalities. The enzyme's malfunction may also relate to liver diseases as improper breakdown of glucuronides can impact liver function. Studies investigate the connection of Beta Glucuronidase with other lysosomal storage disorder proteins to better understand their collective impact on disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Displays beta-glucuronidase activity with the artificial substrate p-nitrophenyl-beta-D-glucuronide (PNPG) and with 4-methylumbelliferyl-glucuronide (PubMed : 21051639, PubMed : 23690068, PubMed : 26364932, PubMed : 3105604, PubMed : 33664385). Is likely capable of scavenging glucuronate from a range of chemically distinct xenobiotic and endobiotic glucuronides present in the gastrointestinal (GI) tract, to be able to utilize these diverse sources of carbon. As part of the GI microbiome, this enzyme is able to reactivate glucuronide drug conjugates, such reactivated compounds can significantly damage the GI tract (PubMed : 26364932).

See full target information uidA

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4100 PubMed40316514

2025

Suppressing an auxin efflux transporter enhances rice adaptation to temperate habitats.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Cui,Lifang Huang,Peng Liu,Xiaodong Wang,Bi Wu,Yongjun Tan,Xuan Huang,Xiaojie Hu,Zhankun He,Yuqi Xia,Zebang Li,Wenli Zhang,Wenbang Tang,Yongzhong Xing,Caiyan Chen,Donghai Mao

Plant biotechnology journal 23:36-50 PubMed39312475

2024

The LCG1-OsBP5/OsEBP89-Wx module regulates the grain chalkiness and taste quality in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Tu,Tao Zhang,Pin Liu,Wen Yang,Ling Zheng,Ying Dai,Hao Wang,Song Lin,Zehua Zhang,Xiaohang Zheng,Mengting Yuan,Yong Chen,Xiaobo Zhu,Hua Yuan,Ting Li,Jiawei Xiong,Zhaohui Zhong,Weilan Chen,Bingtian Ma,Peng Qin,Yuping Wang,Shigui Li

Plant biotechnology journal 22:3361-3379 PubMed39175411

2024

Multiplex Expression Cassette Assembly: A flexible and versatile method for building complex genetic circuits in conventional vectors.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Jiang,Zhuoxiang Zhang,Xiuming Wu,Changmei Li,Xuan Sun,Yiting Li,Aixia Chang,Aiguo Yang,Changqing Yang

Nature communications 14:254 PubMed36650156

2023

Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Mélanie Ormancey,Bruno Guillotin,Rémy Merret,Laurent Camborde,Carine Duboé,Bertrand Fabre,Cécile Pouzet,Francis Impens,Delphi Van Haver,Marie-Christine Carpentier,Hélène San Clemente,Marielle Aguilar,Dominique Lauressergues,Lars B Scharff,Carole Pichereaux,Odile Burlet-Schiltz,Cécile Bousquet-Antonelli,Kris Gevaert,Patrice Thuleau,Serge Plaza,Jean-Philippe Combier

Journal of integrative plant biology 64:23-38 PubMed34783157

2021

Loss of Gn1a/OsCKX2 confers heavy-panicle rice with excellent lodging resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Tu,Zhang Tao,Shiguang Wang,Lei Zhou,Ling Zheng,Chun Zhang,Xinzi Li,Xiaoyu Zhang,Junjie Yin,Xiaobo Zhu,Hua Yuan,Ting Li,Weilan Chen,Peng Qin,Bingtian Ma,Yuping Wang,Shigui Li

Science advances 7: PubMed33523901

2021

Leaf-derived ABA regulates rice seed development via a transporter-mediated and temperature-sensitive mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Qin,Guohua Zhang,Binhua Hu,Jie Wu,Weilan Chen,Zhijie Ren,Yulan Liu,Jing Xie,Hua Yuan,Bin Tu,Bingtian Ma,Yuping Wang,Limin Ye,Legong Li,Chengbin Xiang,Shigui Li

Nature communications 11:5118 PubMed33046692

2020

TERMINAL FLOWER 1-FD complex target genes and competition with FLOWERING LOCUS T.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhu,Samantha Klasfeld,Cheol Woong Jeong,Run Jin,Koji Goto,Nobutoshi Yamaguchi,Doris Wagner

Plants (Basel, Switzerland) 9: PubMed32823972

2020

An AC-Rich Bean Element Serves as an Ethylene-Responsive Element in .

Applications

Unspecified application

Species

Unspecified reactive species

Chunying Wang,Tingting Lin,Mengqi Wang,Xiaoting Qi

Molecular plant 9:221-232 PubMed26455463

2015

Two Tightly Linked Genes at the hsa1 Locus Cause Both F1 and F2 Hybrid Sterility in Rice.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiko Kubo,Tomonori Takashi,Motoyuki Ashikari,Atsushi Yoshimura,Nori Kurata

Nature communications 5:3476 PubMed24647160

2014

Jasmonic acid regulates spikelet development in rice.

Applications

Species

Unspecified reactive species

Qiang Cai,Zheng Yuan,Mingjiao Chen,Changsong Yin,Zhijing Luo,Xiangxiang Zhao,Wanqi Liang,Jianping Hu,Dabing Zhang

View all publications

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Product promise

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Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise