Rabbit Polyclonal beta glucuronidase (GUSB) antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GUSB.
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Publications
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- FEBS letters 592:1366-13772018Mannose 6-phosphate-dependent lysosomal enzyme targeting in hydra: a biochemical, immunological and structural elucidation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPoorna Manasa Bhamidimarri et. al.PubMed 29537487
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GUSB. The exact immunogen used to generate this antibody is proprietary information. Database link P08236
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plays an important role in the degradation of dermatan and keratan sulfates.
Alternative names
Beta-glucuronidase, Beta-G1, GUSB
Recommended products
Rabbit Polyclonal beta glucuronidase (GUSB) antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GUSB.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GUSB. The exact immunogen used to generate this antibody is proprietary information. Database link P08236
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab136860 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta glucuronidase also known as GUSB is an enzyme with several well-known names including b glucuronidase b-glucuronidase and beta-glucuronidase. It is a hydrolase enzyme with a molecular mass around 75 kDa. The enzyme is widely expressed in lysosomes of various tissues such as liver spleen and kidney. Mechanically beta-glucuronidase cleaves beta-D-glucuronic acid residues from glycosaminoglycans steroids and hormones an essential step for the degradation of glycosaminoglycans.
- Biological function summary
Beta glucuronidase performs significant roles in cellular metabolism and molecule recycling. This enzyme aids in the breakdown and recycling of complex carbohydrates. By facilitating hydrolysis it helps in maintaining cellular homeostasis. Beta glucuronidase is not part of a complex; it functions independently within lysosomes. Its activity is vital for recycling endocytosed material and managing cellular turnover.
- Pathways
Beta glucuronidase plays an important role in the lysosomal degradation pathway. This enzyme aids in glycosaminoglycan catabolism a process important for cellular waste disposal and recycling. Its activity connects with several proteins in the pathway notably those involved in lysosomal function such as Hexosaminidase. Beta glucuronidase's enzymatic function further integrates into the broader context of carbohydrate metabolism pathways enabling efficient breakdown of large carbohydrate molecules.
- Associated diseases and disorders
Mutations or deficiencies in beta glucuronidase lead to mucopolysaccharidosis type VII also called Sly syndrome. This lysosomal storage disorder results in the accumulation of undegraded glycosaminoglycans causing developmental delay skeletal abnormalities and other systemic issues. Additionally beta glucuronidase contributes to cancer biology where altered expressions can influence tumor progression. Proteins like glucocerebrosidase may interact indirectly impacting the disease's pathophysiology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-beta glucuronidase (GUSB) antibody (ab136860)
All lanes: Western blot - Anti-beta glucuronidase (GUSB) antibody (ab136860) at 1/500 dilution
All lanes: COLO205 cell lysate at 30 µg
Predicted band size: 75 kDa
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