Rabbit Polyclonal beta glucuronidase (GUSB) antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GUSB aa 100-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GUSB aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link P08236
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Plays an important role in the degradation of dermatan and keratan sulfates.
Alternative names
Beta-glucuronidase, Beta-G1, GUSB
Recommended products
Rabbit Polyclonal beta glucuronidase (GUSB) antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GUSB aa 100-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GUSB aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link P08236
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta glucuronidase also known as GUSB is an enzyme with several well-known names including b glucuronidase b-glucuronidase and beta-glucuronidase. It is a hydrolase enzyme with a molecular mass around 75 kDa. The enzyme is widely expressed in lysosomes of various tissues such as liver spleen and kidney. Mechanically beta-glucuronidase cleaves beta-D-glucuronic acid residues from glycosaminoglycans steroids and hormones an essential step for the degradation of glycosaminoglycans.
- Biological function summary
Beta glucuronidase performs significant roles in cellular metabolism and molecule recycling. This enzyme aids in the breakdown and recycling of complex carbohydrates. By facilitating hydrolysis it helps in maintaining cellular homeostasis. Beta glucuronidase is not part of a complex; it functions independently within lysosomes. Its activity is vital for recycling endocytosed material and managing cellular turnover.
- Pathways
Beta glucuronidase plays an important role in the lysosomal degradation pathway. This enzyme aids in glycosaminoglycan catabolism a process important for cellular waste disposal and recycling. Its activity connects with several proteins in the pathway notably those involved in lysosomal function such as Hexosaminidase. Beta glucuronidase's enzymatic function further integrates into the broader context of carbohydrate metabolism pathways enabling efficient breakdown of large carbohydrate molecules.
- Associated diseases and disorders
Mutations or deficiencies in beta glucuronidase lead to mucopolysaccharidosis type VII also called Sly syndrome. This lysosomal storage disorder results in the accumulation of undegraded glycosaminoglycans causing developmental delay skeletal abnormalities and other systemic issues. Additionally beta glucuronidase contributes to cancer biology where altered expressions can influence tumor progression. Proteins like glucocerebrosidase may interact indirectly impacting the disease's pathophysiology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody (ab244453)
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human liver tissue labeling GUSB with ab244453 at 1/500 dilution.
Western blot - Anti-beta glucuronidase (GUSB) antibody (ab244453)
All lanes: Western blot - Anti-beta glucuronidase (GUSB) antibody (ab244453) at 0.4 µg/mL
Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2: beta glucuronidase (GUSB) over-expression HEK-293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 75 kDa
Immunocytochemistry/ Immunofluorescence - Anti-beta glucuronidase (GUSB) antibody (ab244453)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for beta glucuronidase (GUSB) (green) using ab244453 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody (ab244453)
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human Endometrium tissue labeling GUSB with ab244453 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody (ab244453)
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human Prostate tissue labeling GUSB with ab244453 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody (ab244453)
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human Prostate tissue labeling GUSB with ab244453 at 1/500 dilution.
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