Anti-beta I Tubulin antibody [EPR16778]
- RabMAb
- Recombinant
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(26 Publications)
Rabbit Recombinant Monoclonal TBB1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Chicken, Human, Hamster, Dog, Cow, African green monkey samples. Cited in 26 publications.
View Alternative Names
Tubulin beta-1 chain, TUBB1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on germinal center lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Intracellular flow cytometric analysis of2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% TritonX-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/500 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/400 dilution (green). Cytoplasm and nuclear staining on K562 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab179511 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on follicular center lymphocytes of Rat spleen tissue is observed. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on neuron cells of Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Negative control : Using PBS instead of primary ab secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/20000 dilution
Lane 1:
K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 20 µg
Lane 2:
Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg
Lane 3:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg
Lane 4:
293T (Human epithelial cells from embryonic kidney) whole cell lysates at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
false
- WB
Supplier Data
Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution
Lane 1:
Human fetal brain lysate at 10 µg
Lane 2:
Human fetal heart lysate at 10 µg
Lane 3:
Human fetal kidney lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
false
- WB
Supplier Data
Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution
Lane 1:
C6 (Rat glial tumor cells) whole cell lysates at 20 µg
Lane 2:
RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 20 µg
Lane 3:
PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg
Lane 4:
NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
false
- WB
Supplier Data
Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution
Lane 1:
Mouse brain lysate at 10 µg
Lane 2:
Rat brain lysate at 10 µg
Lane 3:
Rat spleen lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
false
- WB
Supplier Data
Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/1000 dilution
Lane 1:
UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 20 µg
Lane 2:
BHK (Hamster kidney fibroblast cells) whole cell lysates at 20 µg
Lane 3:
MDCK (Canine kidney cell line) whole cell lysates at 20 µg
Lane 4:
MDBK (Bovine kidney cell line) whole cell lysates at 20 µg
Lane 5:
COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
false
Related conjugates and formulations (2)
-
Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-beta I Tubulin antibody [EPR16778]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beta tubulins are important for proper cell division signal transduction and organelle positioning. Beta I Tubulin integrates into the microtubule complex playing an important role in platelet formation and function. The dynamic behavior of microtubules facilitated by beta I Tubulin and its interactions influences cytoskeletal organization enabling cells to maintain their architecture and transport intracellular components effectively.
Pathways
Beta I Tubulin becomes critical in signaling pathways that involve cytoskeleton dynamics such as the Rho GTPase signaling pathway which is essential for actin and microtubule dynamics. It works together with proteins like alpha-tubulin and actin to ensure the proper development of megakaryocytes precursors to platelets. The function of these pathways determines efficient platelet production and stabilization closely linked to overall cellular homeostasis and response to external stimuli.
Product protocols
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Target data
Publications (26)
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Scientific reports 15:33150 PubMed41006496
2025
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Leukemia 39:2789-2800 PubMed40813622
2025
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Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e14595 PubMed39985481
2025
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Cancer & metabolism 12:30 PubMed39456039
2024
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The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39299804
2024
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Infection and immunity 92:e0017224 PubMed39207146
2024
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Nucleic acids research 52:9886-9903 PubMed38943343
2024
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Gynecologic and obstetric investigation 90:42-54 PubMed38934184
2024
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Experimental and therapeutic medicine 27:268 PubMed38756904
2024
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Cancer science 115:1154-1169 PubMed38278779
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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