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AB179511

Anti-beta I Tubulin antibody [EPR16778]

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(26 Publications)

Rabbit Recombinant Monoclonal TBB1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Chicken, Human, Hamster, Dog, Cow, African green monkey samples. Cited in 26 publications.

View Alternative Names

Tubulin beta-1 chain, TUBB1

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on germinal center lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% TritonX-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/500 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/400 dilution (green). Cytoplasm and nuclear staining on K562 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab179511 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on follicular center lymphocytes of Rat spleen tissue is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on neuron cells of Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • WB

Supplier Data

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/20000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

Lane 4:

293T (Human epithelial cells from embryonic kidney) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • WB

Supplier Data

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • WB

Supplier Data

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysates at 20 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • WB

Supplier Data

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)
  • WB

Supplier Data

Western blot - Anti-beta I Tubulin antibody [EPR16778] (AB179511)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/1000 dilution

Lane 1:

UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 20 µg

Lane 2:

BHK (Hamster kidney fibroblast cells) whole cell lysates at 20 µg

Lane 3:

MDCK (Canine kidney cell line) whole cell lysates at 20 µg

Lane 4:

MDBK (Bovine kidney cell line) whole cell lysates at 20 µg

Lane 5:

COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

  • Carrier free

    Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-beta I Tubulin antibody [EPR16778]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16778

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, African green monkey

Applications

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta I Tubulin also known as TUBB1 is an essential component of the microtubule cytoskeleton specifically expressed in hematopoietic cells such as platelets. It is a protein with a mass of approximately 50 kDa. This protein shares structural similarities with other beta tubulins forming heterodimers with alpha-tubulin to construct the microtubules necessary for cell shape and movement. Beta I Tubulin primarily localizes to regions where it aids in the dynamics of microtubule assembly and stabilization.
Biological function summary

Beta tubulins are important for proper cell division signal transduction and organelle positioning. Beta I Tubulin integrates into the microtubule complex playing an important role in platelet formation and function. The dynamic behavior of microtubules facilitated by beta I Tubulin and its interactions influences cytoskeletal organization enabling cells to maintain their architecture and transport intracellular components effectively.

Pathways

Beta I Tubulin becomes critical in signaling pathways that involve cytoskeleton dynamics such as the Rho GTPase signaling pathway which is essential for actin and microtubule dynamics. It works together with proteins like alpha-tubulin and actin to ensure the proper development of megakaryocytes precursors to platelets. The function of these pathways determines efficient platelet production and stabilization closely linked to overall cellular homeostasis and response to external stimuli.

Beta I Tubulin has connections to conditions such as congenital macrothrombocytopenia and other platelet disorders. Mutations or dysregulations of beta I Tubulin can lead to abnormal platelet morphology and function impacting hemostasis. This protein also shows connections to alpha-tubulin in these disorders indicating the essential partnership between tubulin subunits in the formation of functional microtubules and emphasizing their significance in maintaining normal physiological states.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
See full target information TUBB1

Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:33150 PubMed41006496

2025

Neonatal sevoflurane exposure enhances stress-related neurological susceptibility via NKCC1 modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Bi-Ying Yuan,Li-Hui Shan,Jiang Zou,Xiao-Hai Xie,Jia-Qi Gan,Ben-Zhen Chen

Leukemia 39:2789-2800 PubMed40813622

2025

Unraveling the impact of crizotinib to promote megakaryopoiesis for alleviating thrombocytopenia in myelodysplastic neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Kobayashi,Yuta Komizo,Nanami Watanabe,Yu Miyata,Yoshiya Ohnuma,Yasushige Kamimura-Aoyagi,Kanako Yuki,Yoshihiro Hayashi,Minoru Yoshida,Yuka Harada,Hironori Harada

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e14595 PubMed39985481

2025

Human herpesvirus-associated transposable element activation in human aging brains with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yayan Feng,Shu-Qin Cao,Yi Shi,Anna Sun,Margaret E Flanagan,James B Leverenz,Andrew A Pieper,Jae U Jung,Jeffrey Cummings,Evandro Fei Fang,Pengyue Zhang,Feixiong Cheng

Cancer & metabolism 12:30 PubMed39456039

2024

RNF2 promotes chondrosarcoma progression by regulating ubiquitination and degradation of CBX7.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wu,Zheng Huang,Ping Luo,Zhong Xiang,Meng Zhang,Zhiwu Chen,Yalu Zhou,Jiameng Li

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39299804

2024

Glucocorticoids Rapidly Modulate Ca1.2-Mediated Calcium Signals through Kv2.1 Channel Clusters in Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wan,Tongchuang Lu,Chenyang Li,Changlong Hu

Infection and immunity 92:e0017224 PubMed39207146

2024

alleviates abdominal aortic aneurysms via restoring CITED2 activated by EPAS1.

Applications

Unspecified application

Species

Unspecified reactive species

Siqing Wang,Hang Shi,Yue Cheng,Lei Jiang,Yang Lou,Manish Kumar,Mingfei Sun,Xianze Shao,Xuan Zhao,Baichun Wang

Nucleic acids research 52:9886-9903 PubMed38943343

2024

Phosphorylation of the nuclear poly(A) binding protein (PABPN1) during mitosis protects mRNA from hyperadenylation and maintains transcriptome dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Jackson M Gordon,David V Phizicky,Leonard Schärfen,Courtney L Brown,Dahyana Arias Escayola,Jean Kanyo,TuKiet T Lam,Matthew D Simon,Karla M Neugebauer

Gynecologic and obstetric investigation 90:42-54 PubMed38934184

2024

METTL14 Promotes Proliferation, Migration, and Invasion in Endometriotic Stromal Cell Growth by Activating the ZEB1/MEK/ERK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Lv,Fang Li

Experimental and therapeutic medicine 27:268 PubMed38756904

2024

CB1 receptor mediates anesthetic drug ketamine‑induced neuroprotection against glutamate in HT22 cells.

Applications

Unspecified application

Species

Unspecified reactive species

He Bao,Chen Wang,Xiaorong Xue,Bin Hu,Qi Guo

Cancer science 115:1154-1169 PubMed38278779

2024

Itraconazole inhibits tumor growth via CEBPB-mediated glycolysis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhang,Lu Li,Feifei Chu,Huili Wu,Xingguo Xiao,Jianping Ye,Kunkun Li
View all publications

Product promise

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