Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)

Rabbit Recombinant Monoclonal TBB1 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF, WB and reacts with African green monkey, Human, Mouse, Rat, Hamster, Chicken, Cow, Dog samples. Cited in 1 publication.

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Images

Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (AB250104), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt (Intra)	ICC/IF	WB
Human	Predicted	Tested	Tested	Tested
Mouse	Predicted	Expected	Expected	Tested
Rat	Predicted	Expected	Expected	Tested
African green monkey	Expected	Expected	Expected	Tested
Chicken	Predicted	Expected	Expected	Tested
Cow	Predicted	Expected	Expected	Tested
Dog	Predicted	Expected	Expected	Tested
Hamster	Predicted	Expected	Expected	Tested
Monkey	Predicted	Predicted	Predicted	Predicted

Expected
Expected

Species	Dilution info	Notes
Species African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Monkey	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Hamster, Chicken, Cow, Dog, African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Monkey	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Hamster, Chicken, Cow, Dog, African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Monkey	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Hamster	Dilution info -	Notes -
Species Chicken	Dilution info -	Notes -
Species Human	Dilution info -	Notes -
Species Cow	Dilution info -	Notes -
Species Dog	Dilution info -	Notes -
Species African green monkey	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Monkey	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information TUBB1

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

Tubulin beta-1 chain, TUBB1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR16778
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab250104 is the carrier-free version of Anti-beta I Tubulin antibody [EPR16778] ab179511.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta I Tubulin also known as TUBB1 is an essential component of the microtubule cytoskeleton specifically expressed in hematopoietic cells such as platelets. It is a protein with a mass of approximately 50 kDa. This protein shares structural similarities with other beta tubulins forming heterodimers with alpha-tubulin to construct the microtubules necessary for cell shape and movement. Beta I Tubulin primarily localizes to regions where it aids in the dynamics of microtubule assembly and stabilization.

Biological function summary

Beta tubulins are important for proper cell division signal transduction and organelle positioning. Beta I Tubulin integrates into the microtubule complex playing an important role in platelet formation and function. The dynamic behavior of microtubules facilitated by beta I Tubulin and its interactions influences cytoskeletal organization enabling cells to maintain their architecture and transport intracellular components effectively.

Pathways

Beta I Tubulin becomes critical in signaling pathways that involve cytoskeleton dynamics such as the Rho GTPase signaling pathway which is essential for actin and microtubule dynamics. It works together with proteins like alpha-tubulin and actin to ensure the proper development of megakaryocytes precursors to platelets. The function of these pathways determines efficient platelet production and stabilization closely linked to overall cellular homeostasis and response to external stimuli.

Associated diseases and disorders

Beta I Tubulin has connections to conditions such as congenital macrothrombocytopenia and other platelet disorders. Mutations or dysregulations of beta I Tubulin can lead to abnormal platelet morphology and function impacting hemostasis. This protein also shows connections to alpha-tubulin in these disorders indicating the essential partnership between tubulin subunits in the formation of functional microtubules and emphasizing their significance in maintaining normal physiological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-beta I Tubulin antibody [EPR16778] (Anti-beta I Tubulin antibody [EPR16778] ab179511) at 1/20000 dilution
Lane 1: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 20 µg
Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg
Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg
Lane 4: 293T (Human epithelial cells from embryonic kidney) whole cell lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-beta I Tubulin antibody [EPR16778] (Anti-beta I Tubulin antibody [EPR16778] ab179511) at 1/2000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Human fetal kidney lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-beta I Tubulin antibody [EPR16778] (Anti-beta I Tubulin antibody [EPR16778] ab179511) at 1/2000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Rat brain lysate at 10 µg
Lane 3: Rat spleen lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-beta I Tubulin antibody [EPR16778] (Anti-beta I Tubulin antibody [EPR16778] ab179511) at 1/2000 dilution
Lane 1: C6 (Rat glial tumor cells) whole cell lysates at 20 µg
Lane 2: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 20 µg
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg
Lane 4: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-beta I Tubulin antibody [EPR16778] (Anti-beta I Tubulin antibody [EPR16778] ab179511) at 1/1000 dilution
Lane 1: UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 20 µg
Lane 2: BHK (Hamster kidney fibroblast cells) whole cell lysates at 20 µg
Lane 3: MDCK (Canine kidney cell line) whole cell lysates at 20 µg
Lane 4: MDBK (Bovine kidney cell line) whole cell lysates at 20 µg
Lane 5: COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with Anti-beta I Tubulin antibody [EPR16778] ab179511 at 1/500 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/400 dilution (green). Cytoplasm and nuclear staining on K562 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. Anti-beta I Tubulin antibody [EPR16778] ab179511 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling beta I Tubulin with Anti-beta I Tubulin antibody [EPR16778] ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on germinal center lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta I Tubulin with Anti-beta I Tubulin antibody [EPR16778] ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on neuron cells of Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling beta I Tubulin with Anti-beta I Tubulin antibody [EPR16778] ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on follicular center lymphocytes of Rat spleen tissue is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
This data was developed using Anti-beta I Tubulin antibody [EPR16778] ab179511, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with Anti-beta I Tubulin antibody [EPR16778] ab179511 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.