AB255982

Anti-beta II Tubulin antibody [7B9] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal TBB2A antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples.

View Alternative Names

TUBB2, TUBB2A, Tubulin beta-2A chain, Tubulin beta class IIa

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-87 MG cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

Unknown

Immunoprecipitation - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

beta II Tubulin was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10μg with ab28035 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab28035 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.

Lane 1 : Neuro-2a whole cell lysate 10μg.

Lane 2 : ab28035 IP in Neuro-2a whole cell lysate.

Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab28035 in Neuro-2a whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

All lanes:

Immunoprecipitation - Anti-beta II Tubulin antibody [7B9] (<a href='/en-us/products/primary-antibodies/beta-ii-tubulin-antibody-7b9-ab28035'>ab28035</a>)

Predicted band size: 50 kDa

false

Lab

Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : Human heart (PMID : 20191564).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (<a href='/en-us/products/primary-antibodies/beta-ii-tubulin-antibody-7b9-ab28035'>ab28035</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false

Exposure time: 1s

Lab

Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (<a href='/en-us/products/primary-antibodies/beta-ii-tubulin-antibody-7b9-ab28035'>ab28035</a>) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Y79 (human retinoblastoma retinoblastoma), whole cell lysate at 20 µg

Lane 3:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (<a href='/en-us/products/primary-antibodies/beta-ii-tubulin-antibody-7b9-ab28035'>ab28035</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false

Lab

Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab28035).

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (<a href='/en-us/products/primary-antibodies/beta-ii-tubulin-antibody-7b9-ab28035'>ab28035</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false

Exposure time: 1s

Unconjugated

Anti-beta II Tubulin antibody [7B9]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7B9

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" } } }

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Product details

ab255982 is the carrier-free version of ab28035.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta II tubulin also known as beta 2 tubulin is a significant component of microtubules which are essential for maintaining cell structure and integrity. This protein with an approximate molecular mass of 50 kDa is encoded by the TUBB2B gene. It is expressed in various tissues with high expression levels in neuronal tissues contributing to the formation of the cytoskeleton by polymerizing with alpha tubulin to form heterodimers. Alternative names for this protein include 7b9 and C#7b9 though beta II is a common reference in scientific literature.

Biological function summary

Beta II tubulin plays an important role in cellular processes like cell division and intracellular transport. As part of the microtubule complex it provides critical support in mitotic spindle formation facilitating chromosomal segregation during mitosis. It also assists in maintaining neuronal cell stability which is vital for proper neurological functions. The dynamic nature of microtubules aided by beta II tubulin ensures the correct distribution of organelles and other intracellular structures highlighting its importance in cellular mechanics.

Pathways

Beta II tubulin interacts with several critical cellular pathways including the mitotic spindle checkpoint pathway and axonal guidance. It closely associates with other tubulins such as alpha and gamma tubulin ensuring the regulation and stabilization of microtubule dynamics. These pathways are integral to cell cycle regulation and neural development highlighting beta II tubulin's involvement in important cellular and developmental processes.

Mutations or dysregulation in beta II tubulin can relate to neurological disorders such as malformation of cortical development and certain types of brain atrophy. These are often linked to the protein's key role in neuronal function and axonal transport. Additionally altered expression of beta II tubulin has a connection to microtubule-related proteins such as tau which are implicated in neurodegenerative disorders like Alzheimer's disease. This highlights the importance of beta II tubulin in maintaining neuronal health and provides potential targets for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB2A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta II Tubulin antibody [7B9] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Immunoprecipitation - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Western blot - Anti-beta II Tubulin antibody [7B9] - BSA and Azide free (AB255982)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise