Rabbit Polyclonal TBB4B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 24 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Tubulin beta-4B chain aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected |
Rat | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Monkey | Predicted | Predicted | Predicted |
Xenopus laevis | Predicted | Predicted | Predicted |
Xenopus tropicalis | Predicted | Predicted | Predicted |
Zebrafish | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/5000 - 1/20000 | Notes - |
Species Human | Dilution info 1/5000 - 1/20000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Select an associated product type
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
TUBB2C, TUBB4B, TUBB2C, Tubulin beta-4B chain, Tubulin beta-2 chain, Tubulin beta-2C chain
Rabbit Polyclonal TBB4B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 24 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Tubulin beta-4B chain aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Beta II tubulin also known as beta 2 tubulin is a significant component of microtubules which are essential for maintaining cell structure and integrity. This protein with an approximate molecular mass of 50 kDa is encoded by the TUBB2B gene. It is expressed in various tissues with high expression levels in neuronal tissues contributing to the formation of the cytoskeleton by polymerizing with alpha tubulin to form heterodimers. Alternative names for this protein include 7b9 and C#7b9 though beta II is a common reference in scientific literature.
Beta II tubulin plays an important role in cellular processes like cell division and intracellular transport. As part of the microtubule complex it provides critical support in mitotic spindle formation facilitating chromosomal segregation during mitosis. It also assists in maintaining neuronal cell stability which is vital for proper neurological functions. The dynamic nature of microtubules aided by beta II tubulin ensures the correct distribution of organelles and other intracellular structures highlighting its importance in cellular mechanics.
Beta II tubulin interacts with several critical cellular pathways including the mitotic spindle checkpoint pathway and axonal guidance. It closely associates with other tubulins such as alpha and gamma tubulin ensuring the regulation and stabilization of microtubule dynamics. These pathways are integral to cell cycle regulation and neural development highlighting beta II tubulin's involvement in important cellular and developmental processes.
Mutations or dysregulation in beta II tubulin can relate to neurological disorders such as malformation of cortical development and certain types of brain atrophy. These are often linked to the protein's key role in neuronal function and axonal transport. Additionally altered expression of beta II tubulin has a connection to microtubule-related proteins such as tau which are implicated in neurodegenerative disorders like Alzheimer's disease. This highlights the importance of beta II tubulin in maintaining neuronal health and provides potential targets for therapeutic interventions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Terms & Conditions.
10% SDS PAGE
All lanes: Western blot - Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution
All lanes: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa
Paraffin-embedded human breast cancer tissue stained beta II tubuin using ab151318 at 1/250 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Immunofluorescence analysis of 4% paraformaldehyde fixed (at RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling beta II tubulin at cytoskeleton (green) using ab151318 at 1/500 dilution. Blue: Fluoroshield with DAPI. Scale bar= 10 μm.
10% SDS-PAGE
All lanes: Western blot - Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa
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