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Rabbit Polyclonal TBB4B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 24 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Tubulin beta-4B chain aa 200 to C-terminus.

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Images

Western blot - Anti-beta II Tubulin antibody (AB151318), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody (AB151318), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody (AB151318), expandable thumbnail
  • Western blot - Anti-beta II Tubulin antibody (AB151318), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Tubulin beta-4B chain aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P68371

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/5000 - 1/20000

Notes

-

Species

Human

Dilution info

1/5000 - 1/20000

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

Recommended products

Rabbit Polyclonal TBB4B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 24 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Tubulin beta-4B chain aa 200 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Tubulin beta-4B chain aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P68371
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta II tubulin also known as beta 2 tubulin is a significant component of microtubules which are essential for maintaining cell structure and integrity. This protein with an approximate molecular mass of 50 kDa is encoded by the TUBB2B gene. It is expressed in various tissues with high expression levels in neuronal tissues contributing to the formation of the cytoskeleton by polymerizing with alpha tubulin to form heterodimers. Alternative names for this protein include 7b9 and C#7b9 though beta II is a common reference in scientific literature.

Biological function summary

Beta II tubulin plays an important role in cellular processes like cell division and intracellular transport. As part of the microtubule complex it provides critical support in mitotic spindle formation facilitating chromosomal segregation during mitosis. It also assists in maintaining neuronal cell stability which is vital for proper neurological functions. The dynamic nature of microtubules aided by beta II tubulin ensures the correct distribution of organelles and other intracellular structures highlighting its importance in cellular mechanics.

Pathways

Beta II tubulin interacts with several critical cellular pathways including the mitotic spindle checkpoint pathway and axonal guidance. It closely associates with other tubulins such as alpha and gamma tubulin ensuring the regulation and stabilization of microtubule dynamics. These pathways are integral to cell cycle regulation and neural development highlighting beta II tubulin's involvement in important cellular and developmental processes.

Associated diseases and disorders

Mutations or dysregulation in beta II tubulin can relate to neurological disorders such as malformation of cortical development and certain types of brain atrophy. These are often linked to the protein's key role in neuronal function and axonal transport. Additionally altered expression of beta II tubulin has a connection to microtubule-related proteins such as tau which are implicated in neurodegenerative disorders like Alzheimer's disease. This highlights the importance of beta II tubulin in maintaining neuronal health and provides potential targets for therapeutic interventions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-beta II Tubulin antibody (ab151318), expandable thumbnail

    Western blot - Anti-beta II Tubulin antibody (ab151318)

    10% SDS PAGE

    All lanes: Western blot - Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution

    All lanes: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody (ab151318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody (ab151318)

    Paraffin-embedded human breast cancer tissue stained beta II tubuin using ab151318 at 1/250 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody (ab151318), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody (ab151318)

    Immunofluorescence analysis of 4% paraformaldehyde fixed (at RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling beta II tubulin at cytoskeleton (green) using ab151318 at 1/500 dilution. Blue: Fluoroshield with DAPI. Scale bar= 10 μm.

  • Western blot - Anti-beta II Tubulin antibody (ab151318), expandable thumbnail

    Western blot - Anti-beta II Tubulin antibody (ab151318)

    10% SDS-PAGE

    All lanes: Western blot - Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 50 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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