AB151318

Anti-beta II Tubulin antibody

Name: Anti-beta II Tubulin antibody (ab151318) | Abcam
Brand: Abcam Limited
SKU: AB151318

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(26 Publications)

Rabbit Polyclonal TBB4B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 26 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TUBB4B aa 200 to C-terminus.

View Alternative Names

TUBB2C, TUBB4B, Tubulin beta-4B chain, Tubulin beta-2 chain, Tubulin beta-2C chain

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody (AB151318)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody (AB151318)

Paraffin-embedded human breast cancer tissue stained beta II tubuin using ab151318 at 1/250 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody (AB151318)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody (AB151318)

Immunofluorescence analysis of 4% paraformaldehyde fixed (at RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling beta II tubulin at cytoskeleton (green) using ab151318 at 1/500 dilution. Blue : Fluoroshield with DAPI. Scale bar= 10 μm.

Supplier Data

Western blot - Anti-beta II Tubulin antibody (AB151318)

10% SDS-PAGE

All lanes:

Western blot - Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Unknown

Western blot - Anti-beta II Tubulin antibody (AB151318)

10% SDS PAGE

All lanes:

Western blot - Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution

All lanes:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TUBB4B aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P68371

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta II tubulin also known as beta 2 tubulin is a significant component of microtubules which are essential for maintaining cell structure and integrity. This protein with an approximate molecular mass of 50 kDa is encoded by the TUBB2B gene. It is expressed in various tissues with high expression levels in neuronal tissues contributing to the formation of the cytoskeleton by polymerizing with alpha tubulin to form heterodimers. Alternative names for this protein include 7b9 and C#7b9 though beta II is a common reference in scientific literature.

Biological function summary

Beta II tubulin plays an important role in cellular processes like cell division and intracellular transport. As part of the microtubule complex it provides critical support in mitotic spindle formation facilitating chromosomal segregation during mitosis. It also assists in maintaining neuronal cell stability which is vital for proper neurological functions. The dynamic nature of microtubules aided by beta II tubulin ensures the correct distribution of organelles and other intracellular structures highlighting its importance in cellular mechanics.

Pathways

Beta II tubulin interacts with several critical cellular pathways including the mitotic spindle checkpoint pathway and axonal guidance. It closely associates with other tubulins such as alpha and gamma tubulin ensuring the regulation and stabilization of microtubule dynamics. These pathways are integral to cell cycle regulation and neural development highlighting beta II tubulin's involvement in important cellular and developmental processes.

Mutations or dysregulation in beta II tubulin can relate to neurological disorders such as malformation of cortical development and certain types of brain atrophy. These are often linked to the protein's key role in neuronal function and axonal transport. Additionally altered expression of beta II tubulin has a connection to microtubule-related proteins such as tau which are implicated in neurodegenerative disorders like Alzheimer's disease. This highlights the importance of beta II tubulin in maintaining neuronal health and provides potential targets for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB4B

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:388 PubMed40379630

2025

Cytoplasmic connexin43-microtubule interactions promote glioblastoma stem-like cell maintenance and tumorigenicity.

Applications

Unspecified application

Species

Unspecified reactive species

James W Smyth,Sujuan Guo,Lata Chaunsali,Laurie O'Rourke,Jacob Dahlka,Stacie Deaver,Michael Lunski,Elmar Nurmemmedov,Harald Sontheimer,Zhi Sheng,Robert G Gourdie,Samy Lamouille

Clinical and experimental pharmacology & physiology 52:e70027 PubMed39929210

2025

Corticosterone-Induced Myocardial Dysfunctions and the Cardioprotective Role of Tauroursodeoxycholic Acid: An Experimental Study in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Houyuan Zhou,Xiaoying Chen,Yanlin Tao,Zikang Li,Hui Wu,Hailian Shi,Xiaojun Wu,Fei Huang

International journal of molecular sciences 24: PubMed38003549

2023

APOA1 Is a Novel Marker for Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Liu,Jiangnan Pei,Xiaoyue Zhang,Chengjie Wang,Yao Tang,Haiyan Liu,Yi Yu,Shouling Luo,Weirong Gu

Scientific reports 13:14213 PubMed37648686

2023

Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics.

Applications

Unspecified application

Species

Unspecified reactive species

Andrei Riabinin,Ekaterina Kalabusheva,Anastasia Khrustaleva,Mikhail Akulinin,Alexander Tyakht,Egor Osidak,Elina Chermnykh,Andrey Vasiliev,Ekaterina Vorotelyak

Cellular and molecular life sciences : CMLS 79:575 PubMed36309617

2022

Tubb3 expression levels are sensitive to neuronal activity changes and determine microtubule growth and kinesin-mediated transport.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Radwitz,Torben J Hausrat,Frank F Heisler,Philipp C Janiesch,Yvonne Pechmann,Michael Rübhausen,Matthias Kneussel

Developmental neurobiology 81:333-350 PubMed32293117

2020

Alpha- and beta-tubulin isotypes are differentially expressed during brain development.

Applications

Unspecified application

Species

Unspecified reactive species

Torben J Hausrat,Jennifer Radwitz,Franco L Lombino,Petra Breiden,Matthias Kneussel

Molecular pharmacology 96:711-719 PubMed31585985

2019

The Natural Compound Withaferin A Covalently Binds to Cys239 of -Tubulin to Promote Tubulin Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhong Yang,Wei Yan,Yong Li,Lu Niu,Haoyu Ye,Lijuan Chen

Neurochemical research 44:2044-2056 PubMed31278631

2019

Licorisoflavan A Exerts Antidepressant-Like Effect in Mice: Involvement of BDNF-TrkB Pathway and AMPA Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Xiao,Li Liu,Yuanjie Li,Jie Ruan,Hanqing Wang

Child's nervous system : ChNS : official journal o 35:1303-1311 PubMed31161266

2019

MiR-361-5p inhibits cell proliferation and induces cell apoptosis in retinoblastoma by negatively regulating CLDN8.

Applications

Unspecified application

Species

Unspecified reactive species

Bingqian Liu,Baiyang Lu,Xue Wang,Haitao Jiang,Weiwei Kuang

Journal of molecular neuroscience : MN 69:123-132 PubMed31127537

2019

Potential Roles of miR-374a-5p in Mediating Neuroprotective Effects and Related Molecular Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Jiang,Min Yang,Chuyan Wu,Jimei Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta II Tubulin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody (AB151318)

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody (AB151318)

Western blot - Anti-beta II Tubulin antibody (AB151318)

Western blot - Anti-beta II Tubulin antibody (AB151318)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

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